Anti-Histone H3 (mono methyl K56) antibody

• IF

Supplier Data

Immunofluorescence - Anti-Histone H3 (mono methyl K56) antibody (AB272161)

Immunofluorescence analysis of ab272161. HeLa cells were treated by a primary antibody ab272161 at a 1 : 100 dilution for 1 hour at room temperature. It was followed by a secondary antibody Dylight 488 at 1 : 10,000 for 45 minutes at room temprature. Histone H3 [Monomethyl Lys56] localization is nuclear and chromosomal. Histone H3 [Monomethyl Lys56] staining was expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red). Fixation was 0.5% PFA and antigen retrieval was not required.

• WB

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Western blot - Anti-Histone H3 (mono methyl K56) antibody (AB272161)

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa. Other band(s) : None.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K56) antibody (ab272161) at 1 µg/mL

All lanes:

NIH/3T3 cell lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody for 45 min at RT at 1/10000 dilution

Predicted band size: 15 kDa

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• Dot

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Dot Blot - Anti-Histone H3 (mono methyl K56) antibody (AB272161)

Dot Blot analysis of ab272161.

Lane 1 : K56Ac.

Lane 2 : K56Me1.

Lane 3 : K56Me2.

Lane 4 : K56Me3.

Lane 5 : K56 unmodified.

Load : 1, 10, and 100 picomoles of peptide. Primary antibody ab272161 was at 1 : 20-1 : 100 for 45 minutes at 4°C. Dylight™488 rabbit secondary antibody at 1 : 10,000 for 45 minutes at room temperature. Block : 5% BLOTTO overnight at 4°C.