Rabbit Polyclonal H3 mono methyl K79 antibody. Suitable for IP, ChIP, WB, PepArr and reacts with Human, Cow, Mouse, Rat, Synthetic peptide - Human samples. Cited in 52 publications.
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Publications
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- International journal of molecular sciences 24:2023Neuronal Dot1l Activity Acts as a Mitochondrial Gene-Repressor Associated with Human Brain Aging via H3K79 Hypermethylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHendrikus J Van Heesbeen,Lars Von Oerthel,Paul M De Vries,Cindy M R J Wagemans,Marten P SmidtPubMed 36674903
- Journal of medical virology 95:e283242022Infection phase-dependent dynamics of the viral and host N6-methyladenosine epitranscriptome in the lifecycle of an oncogenic virus in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoqing Zhuang,Xuyang Zhao,Jiaxin Jin,Xiaojing Zhu,Rui Wang,Yunyun Zhai,Wenlong Lu,Yifei Liao,Man Teng,Yongxiu Yao,Venugopal Nair,Wen Yao,Aijun Sun,Jun Luo,Gaiping ZhangPubMed 36401345
- PLoS genetics 16:e10091712020DOT-1.1-dependent H3K79 methylation promotes normal meiotic progression and meiotic checkpoint function in C. elegans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura I Lascarez-Lagunas et. al.PubMed 33104701
- Theranostics 10:1758-17762020CBP mediated DOT1L acetylation confers DOT1L stability and promotes cancer metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaohua Liu et. al.PubMed 32042335
- Clinical epigenetics 11:1992019Silencing or inhibition of H3K79 methyltransferase DOT1L induces cell cycle arrest by epigenetically modulating c-Myc expression in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiqun Yang et. al.PubMed 31888761
- Cancers 11:2019The Histone Methyltransferase DOT1L Is a Functional Component of Estrogen Receptor Alpha Signaling in Ovarian Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnamaria Salvati et. al.PubMed 31689915
- Nucleic acids research 47:10977-109932019GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaopeng Lu,Ming Tang,Qian Zhu,Qiaoyan Yang,Zhiming Li,Yantao Bao,Ge Liu,Tianyun Hou,Yafei Lv,Ying Zhao,Haiying Wang,Yang Yang,Zhongyi Cheng,He Wen,Baohua Liu,Xingzhi Xu,Luo Gu,Wei-Guo ZhuPubMed 31612207
- Nature communications 10:38212019Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKwan-Wood Gabriel Lam et. al.PubMed 31444359
- Science advances 5:eaav55902019Inhibition of histone methyltransferase DOT1L silences ERα gene and blocks proliferation of antiestrogen-resistant breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiovanni Nassa et. al.PubMed 30775443
- Molecular cell 73:61-72.e32018Nucleosome Turnover Regulates Histone Methylation Patterns over the Genome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmma J Chory,Joseph P Calarco,Nathaniel A Hathaway,Oliver Bell,Dana S Neel,Gerald R CrabtreePubMed 30472189
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ChIP | WB | PepArr | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected |
| Mouse | Expected | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested | Expected |
| Cow | Expected | Expected | Tested | Expected |
| Synthetic peptide - Human | Not recommended | Not recommended | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg for 106 Cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info 2 µg for 106 Cells | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1.00000 - 1/500.00000 | Notes - |
Species Cow | Dilution info 1/1.00000 - 1/500.00000 | Notes - |
Species Mouse | Dilution info 1/1.00000 - 1/500.00000 | Notes - |
Species Rat | Dilution info 1/1.00000 - 1/500.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
PepArr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 0.02000-0.20000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Cow, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K79me, H3K79me1, H3K79
Recommended products
Rabbit Polyclonal H3 mono methyl K79 antibody. Suitable for IP, ChIP, WB, PepArr and reacts with Human, Cow, Mouse, Rat, Synthetic peptide - Human samples. Cited in 52 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Reacts with Mono-methyl K79 of histone H3. Slight cross-reactivity to di-methyl K79.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
Chromatin was prepared from U-2 OS (Human bone osteosarcoma epithelial cell line) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab2886 (blue), and 20 μl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR. Primers and probes are located in the first kb of the transcribed region.
This image is a courtesy of Anonymous customer reviewChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
Chromatin was prepared from whole cell lysate of normal rat liver and liver cancer cells. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 15 minutes in formaldehyde. 5 μg of the primary antibody was used in 1/100 dilution and it was incubated with the sample for 16 hours at 4°C in a commercially available ChIP dilution buffer. The immunoprecipitated DNA was quantified by real time PCR. ChIP results show that the Histone H3 (mono methyl K79) and GAPDH genes are expressed in higher levels in liver cancer cells than in normal liver cells.
Immunoprecipitation - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
Histone H3 (mono methyl K79) was immunoprecipitated using 0.5mg HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate, 5μg of Rabbit polyclonal to Histone H3 (mono methyl K79) and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2886.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 18kDa: Histone H3 (mono methyl K79).All lanes: Immunoprecipitation - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
Predicted band size: 15 kDa
Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
All lanes: Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886) at 1 µg/mL
All lanes: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human NFIB / NF1B2 peptide (ab95051)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 3min
Peptide Array - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
All batches of ab2886 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - mono methyl K79 peptide (ab4555), indicating that this antibody specifically recognises the Histone H3 - mono methyl K79 modification.
ab4558 - Histone H3 - unmodified
Human Histone H3 (mono methyl K9) peptide ab1771 - Histone H3 - di methyl K9
ab4555 - Histone H3 - mono methyl K79
ab4556 - Histone H3 - di methyl K79
ab4557 - Histone H3 - tri methyl K79
ab4560 - Histone H4 - di methyl K79
Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
All lanes: Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886) at 1/300 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Testis (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 18 kDa
Exposure time: 4min
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