Rabbit Polyclonal H3 mono methyl K9 antibody. Suitable for ChIP, WB, ICC/IF and reacts with Human, Cow samples. Cited in 64 publications.
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- Nature communications 14:74302023Repression of LSD1 potentiates homologous recombination-proficient ovarian cancer to PARP inhibitors through down-regulation of BRCA1/2 and RAD51.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Tao,Yue Zhou,Xiangyu Pan,Yuan Luo,Jiahao Qiu,Xia Zhou,Zhiqian Chen,Yan Li,Lian Xu,Yang Zhou,Zeping Zuo,Chunqi Liu,Liang Wang,Xiaocong Liu,Xinyu Tian,Na Su,Zhengnan Yang,Yu Zhang,Kun Gou,Na Sang,Huan Liu,Jiao Zou,Yuzhou Xiao,Xi Zhong,Jing Xu,Xinyu Yang,Kai Xiao,Yanyang Liu,Shengyong Yang,Yong Peng,Junhong Han,Xiaobo Cen,Yinglan ZhaoPubMed 37973845
- Nature 622:619-6262023Inhibition of fatty acid oxidation enables heart regeneration in adult mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Li,Fan Wu,Stefan Günther,Mario Looso,Carsten Kuenne,Ting Zhang,Marion Wiesnet,Stephan Klatt,Sven Zukunft,Ingrid Fleming,Gernot Poschet,Astrid Wietelmann,Ann Atzberger,Michael Potente,Xuejun Yuan,Thomas BraunPubMed 37758950
- Biology 12:2023TNFα Effects on Adipocytes Are Influenced by the Presence of Lysine Methyltransferases, G9a (EHMT2) and GLP (EHMT1).Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshley A Able,Allison J Richard,Jacqueline M StephensPubMed 37237488
- The EMBO journal 42:e1114732023BRD4-PRC2 represses transcription of T-helper 2-specific negative regulators during T-cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Zhao,Yiqi Wang,Anbalagan Jaganathan,Yifei Sun,Ning Ma,Ning Li,Xinye Han,Xueying Sun,Huanfa Yi,Shibo Fu,Fangbin Han,Xue Li,Kunhong Xiao,Martin J Walsh,Lei Zeng,Ming-Ming Zhou,Ka Lung CheungPubMed 36719036
- Current biology : CB 32:2581-2595.e62022Myotubularin-related phosphatase 5 is a critical determinant of autophagy in neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJason P Chua,Karan Bedi,Michelle T Paulsen,Mats Ljungman,Elizabeth M H Tank,Erin S Kim,Jonathon P McBride,Jennifer M Colón-Mercado,Michael E Ward,Lois S Weisman,Sami J BarmadaPubMed 35580604
- STAR protocols 3:1012432022A cell-sorting-based protocol for cell cycle small-scale ChIP sequencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohnathan R Whetstine,Capucine Van RechemPubMed 35310076
- Nature structural & molecular biology 29:85-962022SETDB1-like MET-2 promotes transcriptional silencing and development independently of its H3K9me-associated catalytic activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColin E Delaney,Stephen P Methot,Veronique Kalck,Jan Seebacher,Daniel Hess,Susan M Gasser,Jan PadekenPubMed 35102319
- Science (New York, N.Y.) 374:eabi74892021The genetic and epigenetic landscape of the centromeres.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew Naish,Michael Alonge,Piotr Wlodzimierz,Andrew J Tock,Bradley W Abramson,Anna Schmücker,Terezie Mandáková,Bhagyshree Jamge,Christophe Lambing,Pallas Kuo,Natasha Yelina,Nolan Hartwick,Kelly Colt,Lisa M Smith,Jurriaan Ton,Tetsuji Kakutani,Robert A Martienssen,Korbinian Schneeberger,Martin A Lysak,Frédéric Berger,Alexandros Bousios,Todd P Michael,Michael C Schatz,Ian R HendersonPubMed 34762468
- Cell reports 37:1097992021Collective regulation of chromatin modifications predicts replication timing during cell cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCapucine Van Rechem,Fei Ji,Damayanti Chakraborty,Joshua C Black,Ruslan I Sadreyev,Johnathan R WhetstinePubMed 34610305
- eLife 10:2021SUV39 SET domains mediate crosstalk of heterochromatic histone marks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlessandro Stirpe,Nora Guidotti,Sarah J Northall,Sinan Kilic,Alexandre Hainard,Oscar Vadas,Beat Fierz,Thomas SchalchPubMed 34524082
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Caenorhabditis elegans | Predicted | Predicted | Predicted |
| Cow | Expected | Tested | Expected |
| Mammals | Predicted | Predicted | Predicted |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-25.00000 µg chromatin | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans, Mammals | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info 0.50000-2.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans, Mammals | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans, Mammals | Dilution info - | Notes - |
Associated Products
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3B, H3FJ, HIST1H3J, H3C3, H3FL, H3C2, HIST1H3A, H3FA, H3C1, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, H3C7, HIST1H3E, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, H3C8, HIST1H3F, H3FI, H3K9me
Recommended products
Rabbit Polyclonal H3 mono methyl K9 antibody. Suitable for ChIP, WB, ICC/IF and reacts with Human, Cow samples. Cited in 64 publications.
Alternative names
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3B, H3FJ, HIST1H3J, H3C3, H3FL, H3C2, HIST1H3A, H3FA, H3C1, H3FC, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, H3C7, HIST1H3E, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, H3C8, HIST1H3F, H3FI, H3K9me
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
Specific for mono-methyl lysine 9 of Histone H3.
There is no cross-reactivity with lysine 27 of Histone H3.- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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8 product images
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
All lanes: Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896) at 1/300 dilution
All lanes: HUH-7 cells at 15 µg
SecondaryAll lanes: Sheep anti-rabbit IgG (HRP) at 1/20000 dilution
Predicted band size: 15 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
All lanes: Diluent: TBS + 0.1% Tween 20 + 5% BSA. Incubated for 12 hours at 4°C
All lanes: HeLa whole cell lysates at 20 µg
SecondaryAll lanes: Anti-rabbit IgG, HRP-linked Antibody at 1/2000 dilution
Predicted band size: 15 kDa
Exposure time: 1min
ChIP - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab8896 (blue), and 20μl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
This image is courtesy of an anonymous customer review.Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
All lanes: Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896) at 1/1000 dilution
Lane 1: Nuclear lysates prepared from Hela cell: SiRNA untreated at 10 µg
Lane 2: Nuclear lysates prepared from Hela cells treated with G9a SiRNA at 10 µg
SecondaryAll lanes: Alkaline Phosphatase conjugated rabbit polyclonal rabbit IgG (Fc) at 1/5000 dilution
Predicted band size: 15 kDa
Observed band size: 16 kDa
Exposure time: 10min
This image is courtesy of a customer review submitted by Kirk Mcmanus.Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
ab8896 staining Histone H3 (mono methyl K9) in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.05% Triton X100 in PBS. Samples were incubated with primary antibody at 1/200 dilution in PBS for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit was used as the secondary antibody at a 1/200 dilution.
Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
ab8896 specifically recognises mono-methyl K9 (lane 1) in calf thymus lysate at 17kDa. ab8896 is successfully blocked using the immunizing peptide (lane 2 Human Histone H3 (mono methyl K9) peptide ab1771), but not the di-methyl K9 (lane 3 Human Histone H3 (di methyl K9) peptide ab1772), the tri-methyl K9 (lane 4 Human Histone H3 (tri methyl K9) peptide ab1773), the mono-methyl K27 (lane 5 Human Histone H3 (mono methyl K27) peptide ab1780), the di-methyl K27 (lane 6 Human Histone H3 (di methyl K27) peptide ab1781), the tri-methyl K27 (lane 7 Human Histone H3 (tri methyl K27) peptide ab1782), the mono-methyl K4 (lane 8 Human Histone H3 (mono methyl K4) peptide ab1340) nor the corresponding unmodified peptides (lane 9 Human Histone H3 (unmodified) peptide ab7228, lane 10 Human Histone H3 (unmodified ) peptide ab2623). This implies that ab8896 is specific to mono-methyl K9.
All lanes: Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896) at 1 µg/mL
All lanes: Calf Thymus Histone Preparation at 0.5 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
ab8896 staining Histone H3 (mono methyl K9) in HeLa (Cervical cancer cell line) cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton/TBS and blocked with 2% serum for 30 minutes at 25°C. Samples were incubated with primary antibody at 1/100 dilution for 1 hour at 25°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit was used as a secondary antibody at a 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (ab8896)
ICC/IF image of ab8896 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8896 at 1μg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was a goat anti-rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.
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