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AB272136

Anti-Histone H3 (mono methyl R2) antibody

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Rabbit Polyclonal H3 mono methyl R2 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human, Caenorhabditis elegans samples. Immunogen corresponding to Synthetic Peptide within Human H3C15.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, H3C1, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3R2me, H3R2me1, H3R2

3 Images
Western blot - Anti-Histone H3 (mono methyl R2) antibody (AB272136)
  • WB

Unknown

Western blot - Anti-Histone H3 (mono methyl R2) antibody (AB272136)

Block : 5% BLOTTO.

All lanes:

Western blot - Anti-Histone H3 (mono methyl R2) antibody (ab272136) at 1/500 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) histone prep lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Western blot - Anti-Histone H3 (mono methyl R2) antibody (AB272136)
  • WB

Unknown

Western blot - Anti-Histone H3 (mono methyl R2) antibody (AB272136)

Block : 5% BLOTTO

All lanes:

Western blot - Anti-Histone H3 (mono methyl R2) antibody (ab272136) at 1/500 dilution

All lanes:

C.elegans embryo lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Dot Blot - Anti-Histone H3 (mono methyl R2) antibody (AB272136)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (mono methyl R2) antibody (AB272136)

Dot Blot analysis of various methylated Histone H3 forms using ab272136 at 1/1000, followed by Dylight™488 rabbit secondary antibody at 1/10000.

Lane 1 : R2 NM.

Lane 2 : R2 Me1.

Lane 3 : R2 Me2s.

Lane 4 : R2 Me2a.

Load : 100, 10, 1 picomoles of peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Caenorhabditis elegans

Applications

WB, Dot

applications

Immunogen

Synthetic Peptide within Human H3C15. The exact immunogen used to generate this antibody is proprietary information.

Q71DI3

Specificity

This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Caenorhabditis elegans": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Anti-Histone H3 [Monomethyl Arg2] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 mono methyl R2
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Product promise

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