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Rabbit Recombinant Monoclonal H3 methyl R63 + asymmetric di methyl R63 + symmetric di methyl R63 antibody. Suitable for WB, PepArr, IHC-P and reacts with Mouse, Human samples.

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Images

Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (AB196724), expandable thumbnail
  • Peptide Array - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (AB196724), expandable thumbnail
  • Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (AB196724), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (AB196724), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBPepArrIHC-P
Human
Tested
Tested
Expected
Mouse
Tested
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Recommended products

Rabbit Recombinant Monoclonal H3 methyl R63 + asymmetric di methyl R63 + symmetric di methyl R63 antibody. Suitable for WB, PepArr, IHC-P and reacts with Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17722
Purification technique
Affinity purification Protein A
Specificity

Detects asymmetric di methyl R63, symmetric di methyl R63, and mono methyl R63.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (ab196724), expandable thumbnail

    Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (ab196724)

    Blocking/Dilution buffer: 5% BSA /TBST.

    All lanes: Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (ab196724) at 1/1000 dilution

    All lanes: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

    Exposure time: 1min

  • Peptide Array - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (ab196724), expandable thumbnail

    Peptide Array - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (ab196724)

    ab196724 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.

  • Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (ab196724), expandable thumbnail

    Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (ab196724)

    Blocking/Dilution buffer: 5% BSA /TBST.

    All lanes: Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (ab196724) at 1/1000 dilution

    All lanes: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (ab196724), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (ab196724)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Histone H3 (methyl R63, asymmetric di methyl R63, symmetric di methyl R63) with ab196724 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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