Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] - BSA and Azide free (AB251224)

This data was developed using ab196724, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Histone H3 (methyl R63, asymmetric di methyl R63, symmetric di methyl R63) with ab196724 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

• WB

Supplier Data

Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] - BSA and Azide free (AB251224)

This data was developed using ab196724, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% BSA /TBST.

All lanes:

Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (<a href='/en-us/products/primary-antibodies/histone-h3-mono-methyl-r63-di-methyl-r63-antibody-epr17722-ab196724'>ab196724</a>) at 1/1000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] - BSA and Azide free (AB251224)

This data was developed using ab196724, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% BSA /TBST.

All lanes:

Western blot - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] (<a href='/en-us/products/primary-antibodies/histone-h3-mono-methyl-r63-di-methyl-r63-antibody-epr17722-ab196724'>ab196724</a>) at 1/1000 dilution

All lanes:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 3min

• PepArr

Supplier Data

Peptide Array - Anti-Histone H3 (mono methyl R63, di methyl R63) antibody [EPR17722] - BSA and Azide free (AB251224)

This data was developed using ab196724, the same antibody clone in a different buffer formulation.

ab196724 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.