Anti-Histone H3 (mono+di+tri methyl K79) antibody [EPR17468] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono+di+tri methyl K79) antibody [EPR17468] - BSA and Azide free (AB240197)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H3 (mono+di+tri methyl K79) using ab177185 at 1/4000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counter stained with Hematoxylin.
Inset image : negative control obtained using PBS instead of ab177185.
Note : Nucleus staining on glandular epithelium of Human colon tissue was observed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177185).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ChIP

Supplier Data

ChIP - Anti-Histone H3 (mono+di+tri methyl K79) antibody [EPR17468] - BSA and Azide free (AB240197)

Chromatin was prepared from HeLa (Human epithelial cells from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab177185 (blue), and 20μl of Anti rabbit IgGsepharose beads. 2μg of Rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (SYBR approach). Primers and probes are located in the first kb of the transcribed region.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177185).

• ChIP-seq

Lab

ChIP-sequencing - Anti-Histone H3 (mono+di+tri methyl K79) antibody [EPR17468] - BSA and Azide free (AB240197)

This data was developed using ab177185, the same antibody clone in a different buffer formulation. Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 4 µg of ab177185 [EPR17468]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. Additional screenshots of mapped reads can be downloaded here.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono+di+tri methyl K79) antibody [EPR17468] - BSA and Azide free (AB240197)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H3 (mono+di+tri methyl K79) using ab177185 at 1/4000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counter stained with Hematoxylin.
Inset image : negative control obtained using PBS instead of ab177185.
Note : Nucleus staining on glandular epithelium of Mouse colon tissue was observed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177185).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mono+di+tri methyl K79) antibody [EPR17468] - BSA and Azide free (AB240197)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H3 (mono+di+tri methyl K79) using ab177185 at 1/4000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counter stained with Hematoxylin.
Inset image : negative control obtained using PBS instead of ab177185.
Note : Nucleus staining on glandular epithelium of Rat colon tissue was observed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177185).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Histone H3 (mono+di+tri methyl K79) antibody [EPR17468] - BSA and Azide free (AB240197)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using ab177185, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Histone H3 (mono+di+tri methyl K79) antibody [EPR17468] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-monoditri-methyl-k79-antibody-epr17468-chip-grade-ab177185'>ab177185</a>) at 1/1000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-Histone H3 (mono+di+tri methyl K79) antibody [EPR17468] - BSA and Azide free (AB240197)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using ab177185, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Histone H3 (mono+di+tri methyl K79) antibody [EPR17468] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-monoditri-methyl-k79-antibody-epr17468-chip-grade-ab177185'>ab177185</a>) at 1/1000 dilution

All lanes:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 1min

• PepArr

Supplier Data

Peptide Array - Anti-Histone H3 (mono+di+tri methyl K79) antibody [EPR17468] - BSA and Azide free (AB240197)

ab177185 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177185).