AB190631

Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade

Name: Anti-histone H3 (mutated K27M) antibody [EPR18340] (ab190631) | Abcam
Brand: Abcam Limited
SKU: AB190631
Price: 620 USD
Availability: InStock
Rating: 5 (4 reviews)

BOND RX™ Validated
RabMAb
Recombinant
20ul selling size
What is this?

(4 Reviews)

(15 Publications)

Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (ab190631) is a rabbit monoclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ELISA, ChIP, Dot Blot. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27M, H3K27

10 Images

ChIP - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

ChIP

Lab

ChIP - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Chromatin was prepared from HEK-293T transfected with myc-His tagged Histone H3 (K27M) and Histone H3 WT cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 2 μg of ab190631 (red), 2 μg of ab213204 (red) (bottom panel, served as internal control) or 2 μg of rabbit normal IgG ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).

Primers and probes are located in the first kb of the transcribed region.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling Histone H3 (K27 mutated to M) with ab190631 at 1/5000 (0.2 ug/ml) dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with myc-tagged H3 (K27 mutated to M) expression vector is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab190631 anti- H3(K27 mutated to M) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of tissue sections from(A) HEK-293T transfected with Histone H3 (mutated K27M) expression vector containing a his tag. (B) HEK-293T transfected with wildtype Histone H3 expression vector containing a his tag. (C) HEK-293T transfected with empty vector containing a his tag, labeling Histone H3 (mutated K27M) with ab190631 at 1/1000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins Positive staining on HEK-293T transfected with Histone H3 (mutated K27M) expression vector cell pellets (image A), no staining on HEK-293T transfected with wildtype Histone H3 expression vector cell pellets (Image B) or empty vector cell pellets (Image C). The section was incubated with ab190631 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Unknown

Immunoprecipitation - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Histone H3 (K27 mutated to M) was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) cells transfected with myc-tagged H3(K27M) expression vector whole cell lysate 10ug with ab190631 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab190631 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293T (human embryonic kidney epithelial cell) cells transfected with myc-tagged H3(K27M) expression vector whole cell lysate 10ug

Lane 2 : ab190631 IP in HEK-293T cells transfected with myc-tagged H3(K27M) expression vector whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab190631 in HEK-293T cells transfected with myc-tagged H3(K27M) expression vector whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds

All lanes:

Immunoprecipitation - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (ab190631)

Predicted band size: 15 kDa

Observed band size: 18 kDa

false

IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of tissue sections from mouse brain tumor with and without H3K27M mutation labeling Histone H3 (mutated K27M) with ab190631 at 1/500 dilution (1 hour at 36°C in 2% BSA in PBS).

Tissue was fixed with 20% Neutral Buffer Formalin for 48 hours, then changed to 70% ethanol for a week. Paraffin-embedded tissue was cut in 5 micrometer thickness with microtome. Antigen retrieval was done with Cell-Conditioning 1 (Roche) at 95 degree. A HRP conjugated monoclonal antibody (DISCOVERY UltraMap anti-Rb HRP (RUO)) was used as the secondary antibody.

This image is courtesy of Dr Yusuke Tomita, Northwestern University.

Lab

Dot Blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Dot blot analysis of Histone H3 (mutated K27M) using ab190631 at 1 : 1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : Histone H3 (K27 mutated to M) peptide
Lane 2 : Histone H3 unmodified peptide
Lane 3 : Histone H3 (tri methyl K18 + tri methyl K36) peptide

All lanes:

Dot Blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (ab190631) at 1/1000 dilution

Lane 1:

H3 (K27 mutated to M) aa22-32

Lane 2:

H3 H3 unmod(1-33)

Lane 3:

H3 K18me3 + K36me3 15-39

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Indirect ELISA - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

I-ELISA

Lab

Indirect ELISA - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

ELISA using ab190631 at varying antibody concentrations and antigen concentration at 1000 ng/mL. An Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

The blue line indicates binding to the Histone H3 (mutated K27M) peptide. Binding to the following peptides was not seen :

Histone H3 WT,
Histone H3 (mono methyl K27),
Histone H3 (di methyl K27),
Histone H3 (tri methyl K27).

This indicates the specificity of ab190631 for mutated K27M of Histone H3.

Supplier Data

Western blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (ab190631) at 1/1000 dilution

Lane 1:

His-tagged recombinant histone H3 K27M protein at 0.01 µg

Lane 2:

His-tagged recombinant wild type histone H3 protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 15s

Lab

Western blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (ab190631) at 1/1000 dilution

All lanes:

HEK-293 transfected with Histone H3.3 (K27M) expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 18 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 6 seconds.

All lanes:

Western blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (ab190631) at 1/1000 dilution

Lane 1:

HEK-293 transfected with Histone H3.1 (K27M) expression vector containing a myc-His-tag, whole cell lysate at 10 µg

Lane 2:

HEK-293 transfected with Histone H3(WT) expression vector containing a myc-His-tag, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 18 kDa

false

Carrier free

Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Histone H3 (mutated K27M) antibody [EPR18340]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18340

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ChIP, IHC-P, Dot, I-ELISA, WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2 µg for 25 µg chromatin", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5000", "ICCIF-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1 µg/mL", "IELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Transfected cell lysate - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

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Product details

Product Specifications
Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (ab190631) is a rabbit recombinant monoclonal antibody and is validated for use in ChIP, Dot Blot, I-ELISA, ICC/IF, IHC-P, IP, WB in human and mouse samples.
Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (ab190631) specifically detects Histone H3 Mutated-K27M (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (ab190631) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (ab190631) has been cited over 12 times in peer reviewed journals and is trusted by the scientific community.
Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (ab190631) has 4 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR18340 - ab240310.
Antibody clone EPR18340 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488 (ab313663).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 mutated K27M

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 8:274 PubMed39587279

2024

Deep mutual learning on hybrid amino acid PET predicts H3K27M mutations in midline gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Yuan,Guanglei Li,Shuhao Mei,Mingtao Hu,Ying-Hua Chu,Yi-Cheng Hsu,Chaolin Li,Jianping Song,Jie Hu,Danyang Feng,Fang Xie,Yihui Guan,Qi Yue,Mianxin Liu,Ying Mao

Proceedings of the National Academy of Sciences of the United States of America 121:e2416614121 PubMed39560642

2024

An E2 ubiquitin-conjugating enzyme links diubiquitinated H2B to H3K27M oncohistone function.

Applications

Unspecified application

Species

Unspecified reactive species

Alan L Jiao,Erdem Sendinc,Barry M Zee,Felice Wallner,Yang Shi

Cancer cell 41:1911-1926.e8 PubMed37802053

2023

TIM-3 blockade in diffuse intrinsic pontine glioma models promotes tumor regression and antitumor immune memory.

Applications

Unspecified application

Species

Unspecified reactive species

Iker Ausejo-Mauleon,Sara Labiano,Daniel de la Nava,Virginia Laspidea,Marta Zalacain,Lucía Marrodán,Marc García-Moure,Marisol González-Huarriz,Irati Hervás-Corpión,Laasya Dhandapani,Silvestre Vicent,Maria Collantes,Iván Peñuelas,Oren J Becher,Mariella G Filbin,Li Jiang,Jenna Labelle,Carlos A O de Biagi-Junior,Javad Nazarian,Sandra Laternser,Timothy N Phoenix,Jasper van der Lugt,Mariette Kranendonk,Raoull Hoogendijk,Sabine Mueller,Carlos De Andrea,Ana C Anderson,Elizabeth Guruceaga,Carl Koschmann,Viveka Nand Yadav,Jaime Gállego Pérez-Larraya,Ana Patiño-García,Fernando Pastor,Marta M Alonso

Nature communications 14:4346 PubMed37468483

2023

Spatially controlled construction of assembloids using bioprinting.

Applications

Unspecified application

Species

Unspecified reactive species

Julien G Roth,Lucia G Brunel,Michelle S Huang,Yueming Liu,Betty Cai,Sauradeep Sinha,Fan Yang,Sergiu P Pașca,Sungchul Shin,Sarah C Heilshorn

Frontiers in oncology 12:1016343 PubMed36568177

2022

Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Lisa Petrilli,Claudia Fuoco,Alessandro Palma,Luca Pasquini,Giulia Pericoli,Yura Grabovska,Alan Mackay,Sabrina Rossi,Angel M Carcaboso,Andrea Carai,Angela Mastronuzzi,Chris Jones,Gianni Cesareni,Franco Locatelli,Maria Vinci

Nature cancer 3:976-993 PubMed35817829

2022

Transition to a mesenchymal state in neuroblastoma confers resistance to anti-GD2 antibody via reduced expression of ST8SIA1.

Applications

Unspecified application

Species

Unspecified reactive species

Nathaniel W Mabe,Min Huang,Guillermo N Dalton,Gabriela Alexe,Daniel A Schaefer,Anna C Geraghty,Amanda L Robichaud,Amy S Conway,Delan Khalid,Marius M Mader,Julia A Belk,Kenneth N Ross,Michal Sheffer,Miles H Linde,Nghi Ly,Winnie Yao,Maria Caterina Rotiroti,Benjamin A H Smith,Marius Wernig,Carolyn R Bertozzi,Michelle Monje,Constantine S Mitsiades,Ravindra Majeti,Ansuman T Satpathy,Kimberly Stegmaier,Robbie G Majzner

Glia 70:1681-1698 PubMed35524725

2022

A novel mouse model of diffuse midline glioma initiated in neonatal oligodendrocyte progenitor cells highlights cell-of-origin dependent effects of H3K27M.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Tomita,Yosuke Shimazu,Agila Somasundaram,Yoshihiro Tanaka,Nozomu Takata,Yukitomo Ishi,Samantha Gadd,Rintaro Hashizume,Angelo Angione,Gonzalo Pinero,Dolores Hambardzumyan,Daniel J Brat,Christine M Hoeman,Oren J Becher

Nature 603:934-941 PubMed35130560

2022

GD2-CAR T cell therapy for H3K27M-mutated diffuse midline gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Robbie G Majzner,Sneha Ramakrishna,Kristen W Yeom,Shabnum Patel,Harshini Chinnasamy,Liora M Schultz,Rebecca M Richards,Li Jiang,Valentin Barsan,Rebecca Mancusi,Anna C Geraghty,Zinaida Good,Aaron Y Mochizuki,Shawn M Gillespie,Angus Martin Shaw Toland,Jasia Mahdi,Agnes Reschke,Esther H Nie,Isabelle J Chau,Maria Caterina Rotiroti,Christopher W Mount,Christina Baggott,Sharon Mavroukakis,Emily Egeler,Jennifer Moon,Courtney Erickson,Sean Green,Michael Kunicki,Michelle Fujimoto,Zach Ehlinger,Warren Reynolds,Sreevidya Kurra,Katherine E Warren,Snehit Prabhu,Hannes Vogel,Lindsey Rasmussen,Timothy T Cornell,Sonia Partap,Paul G Fisher,Cynthia J Campen,Mariella G Filbin,Gerald Grant,Bita Sahaf,Kara L Davis,Steven A Feldman,Crystal L Mackall,Michelle Monje

Cancers 13: PubMed34771714

2021

H3.3K27M Mutation Controls Cell Growth and Resistance to Therapies in Pediatric Glioma Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Andria Rakotomalala,Quentin Bailleul,Clara Savary,Mélanie Arcicasa,Maud Hamadou,Paul Huchedé,Audrey Hochart,Audrey Restouin,Remy Castellano,Yves Collette,Emma Dieny,Audrey Vincent,Pierre-Olivier Angrand,Xuefen Le Bourhis,Pierre Leblond,Alessandro Furlan,Marie Castets,Eddy Pasquier,Samuel Meignan

Nature genetics 53:1221-1232 PubMed34294917

2021

Simultaneous disruption of PRC2 and enhancer function underlies histone H3.3-K27M oncogenic activity in human hindbrain neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gerard L Brien,Raul Bardini Bressan,Craig Monger,Dáire Gannon,Eimear Lagan,Anthony M Doherty,Evan Healy,Hannah Neikes,Darren J Fitzpatrick,Orla Deevy,Vivien Grant,Maria-Angeles Marqués-Torrejón,Neza Alfazema,Steven M Pollard,Adrian P Bracken

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade

ChIP - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Immunoprecipitation - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Dot Blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Indirect ELISA - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Western blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Western blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

Western blot - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (AB190631)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (15)

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