Rabbit Recombinant Monoclonal H3 mutated K27M antibody. Carrier free. Suitable for IHC-P, IP, ChIP, I-ELISA, ICC/IF, WB and reacts with Mouse, Transfected cell lysate, Transfected cell line, Synthetic peptide, Recombinant full length protein samples. Cited in 3 publications.
Images
![ChIP - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (AB240310), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k27m-antibody-epr18340-chip-grade-bsa-and-azide-free-ab240310--chip-img119015.jpg/_jcr_content/renditions/webp-475.webp "ChIP - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (AB240310)")
![Immunoprecipitation - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (AB240310), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k27m-antibody-epr18340-chip-grade-bsa-and-azide-free-ab240310--immunoprecipitation-img138050.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (AB240310)")
![Indirect ELISA - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (AB240310), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k27m-antibody-epr18340-chip-grade-bsa-and-azide-free-ab240310--indirect-elisa-img90553.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (AB240310)")
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (AB240310), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k27m-antibody-epr18340-chip-grade-bsa-and-azide-free-ab240310--immunocytochemistry-immunofluorescence-img64379.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (AB240310)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (AB240310), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k27m-antibody-epr18340-chip-grade-bsa-and-azide-free-ab240310--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422898.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (AB240310)")
Publications
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- Nature genetics 54:1881-18942022The landscape of tumor cell states and spatial organization in H3-K27M mutant diffuse midline glioma across age and location.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlon Liu,Li Jiang,Erik R Samuelsson,Sergio Marco Salas,Alexander Beck,Olivia A Hack,Daeun Jeong,McKenzie L Shaw,Bernhard Englinger,Jenna LaBelle,Hafsa M Mire,Sibylle Madlener,Lisa Mayr,Michael A Quezada,Maria Trissal,Eshini Panditharatna,Kati J Ernst,Jayne Vogelzang,Taylor A Gatesman,Matthew E Halbert,Hana Palova,Petra Pokorna,Jaroslav Sterba,Ondrej Slaby,Rene Geyeregger,Aaron Diaz,Izac J Findlay,Matthew D Dun,Adam Resnick,Mario L Suvà,David T W Jones,Sameer Agnihotri,Jessica Svedlund,Carl Koschmann,Christine Haberler,Thomas Czech,Irene Slavc,Jennifer A Cotter,Keith L Ligon,Sanda Alexandrescu,W K Alfred Yung,Isabel Arrillaga-Romany,Johannes Gojo,Michelle Monje,Mats Nilsson,Mariella G FilbinPubMed 36471067
- Cancer discovery 12:2880-29052022BAF Complex Maintains Glioma Stem Cells in Pediatric H3K27M Glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEshini Panditharatna,Joana G Marques,Tingjian Wang,Maria C Trissal,Ilon Liu,Li Jiang,Alexander Beck,Andrew Groves,Neekesh V Dharia,Deyao Li,Samantha E Hoffman,Guillaume Kugener,McKenzie L Shaw,Hafsa M Mire,Olivia A Hack,Joshua M Dempster,Caleb Lareau,Lingling Dai,Logan H Sigua,Michael A Quezada,Ann-Catherine J Stanton,Meghan Wyatt,Zohra Kalani,Amy Goodale,Francisca Vazquez,Federica Piccioni,John G Doench,David E Root,Jamie N Anastas,Kristen L Jones,Amy Saur Conway,Sylwia Stopka,Michael S Regan,Yu Liang,Hyuk-Soo Seo,Kijun Song,Puspalata Bashyal,William P Jerome,Nathan D Mathewson,Sirano Dhe-Paganon,Mario L Suvà,Angel M Carcaboso,Cinzia Lavarino,Jaume Mora,Quang-De Nguyen,Keith L Ligon,Yang Shi,Sameer Agnihotri,Nathalie Y R Agar,Kimberly Stegmaier,Charles D Stiles,Michelle Monje,Todd R Golub,Jun Qi,Mariella G FilbinPubMed 36305736
- Nucleic acids research 51:1662-16732022PRC2-independent actions of H3.3K27M in embryonic stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLea R Z Cohen,Binyamin Kaffe,Eden Deri,Chen Leibson,Malka Nissim-Rafinia,Moria Maman,Nofar Harpaz,Guy Ron,Efrat Shema,Eran MeshorerPubMed 36156096
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | ChIP | I-ELISA | ICC/IF | WB | |
|---|---|---|---|---|---|---|
| Human | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Mouse | Expected | Predicted | Predicted | Predicted | Predicted | Predicted |
| Recombinant full length protein | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Transfected cell line | Not recommended | Not recommended | Tested | Not recommended | Tested | Not recommended |
| Transfected cell lysate | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Tested |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Transfected cell line, Synthetic peptide, Recombinant full length protein | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line, Synthetic peptide, Recombinant full length protein | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Synthetic peptide, Recombinant full length protein | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Transfected cell line, Recombinant full length protein | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Synthetic peptide, Recombinant full length protein | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate, Recombinant full length protein | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
9 products for Alternative Product
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FL, H3C2, HIST1H3A, H3FA, H3C1, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, HIST1H3F, HIST1H3B, H3C3, H3FC, H3C8, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI
Recommended products
Rabbit Recombinant Monoclonal H3 mutated K27M antibody. Carrier free. Suitable for IHC-P, IP, ChIP, I-ELISA, ICC/IF, WB and reacts with Mouse, Transfected cell lysate, Transfected cell line, Synthetic peptide, Recombinant full length protein samples. Cited in 3 publications.
Alternative names
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FL, H3C2, HIST1H3A, H3FA, H3C1, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, HIST1H3F, HIST1H3B, H3C3, H3FC, H3C8, HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR18340
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab240310 is the carrier-free version of Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![ChIP - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k27m-antibody-epr18340-chip-grade-bsa-and-azide-free-ab240310--chip-img119015.jpg "ChIP - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310)")
ChIP - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310)
This data was developed using Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631, the same antibody clone in a different buffer formulation.
Chromatin was prepared from 293T transfected with myc-His tagged Histone H3(K27 M) and Histone H3 WT cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 2 μg of Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631 (red), 2 μg of Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 (red) (bottom panel, served as internal control) or 2 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).Primers and probes are located in the first kb of the transcribed region.
![Immunoprecipitation - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k27m-antibody-epr18340-chip-grade-bsa-and-azide-free-ab240310--immunoprecipitation-img138050.jpg "Immunoprecipitation - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310)")
Immunoprecipitation - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310)
Histone H3 (K27 mutated to M) was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) cells transfected with myc-tagged H3(K27M) expression vector whole cell lysate 10ug with Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with myc-tagged H3(K27M) expression vector whole cell lysate 10ug
Lane 2: Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631 IP in 293T cells transfected with myc-tagged H3(K27M) expression vector whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631 in 293T cells transfected with myc-tagged H3(K27M) expression vector whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, azide, BSA and glycerol (Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631).
All lanes: Immunoprecipitation - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade (Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631)
Predicted band size: 15 kDa
Observed band size: 18 kDa
![Indirect ELISA - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k27m-antibody-epr18340-chip-grade-bsa-and-azide-free-ab240310--indirect-elisa-img90553.jpg "Indirect ELISA - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310)")
Indirect ELISA - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310)
This data was developed using Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631, the same antibody clone in a different buffer formulation.
ELISA using Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631 at varying antibody concentrations and antigen concentration at 1000 ng/mL. An Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
The blue line indicates binding to the Histone H3 (mutated K27M) peptide. Binding to the following peptides was not seen:
Histone H3 WT,
Histone H3 (mono methyl K27),
Histone H3 (di methyl K27),
Histone H3 (tri methyl K27).This indicates the specificity of Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631 for mutated K27M of Histone H3.
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k27m-antibody-epr18340-chip-grade-bsa-and-azide-free-ab240310--immunocytochemistry-immunofluorescence-img64379.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Histone H3 (K27 mutated to M) with Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631 at 1/5000 (0.2 ug/ml) dilution, followed by Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631 anti- H3(K27 mutated to M) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in 293T cells transfected with myc-tagged H3 (K27 mutated to M) expression vector. is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631 anti- H3(K27 mutated to M) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade ab190631).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k27m-antibody-epr18340-chip-grade-bsa-and-azide-free-ab240310--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422898.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (mutated K27M) antibody [EPR18340] - ChIP Grade – BSA and Azide free (ab240310)
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