Rabbit Recombinant Monoclonal H3 mutated K9 antibody. Carrier free. Suitable for WB, Dot, Flow Cyt (Intra) and reacts with Human, Synthetic peptide - Human, Transfected cell line - Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (AB289994), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k9m-antibody-epr26429-113-bsa-and-azide-free-ab289994--flow-cytometry-intracellular-img171841.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (AB289994)")
![Dot Blot - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (AB289994), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k9m-antibody-epr26429-113-bsa-and-azide-free-ab289994--dot-blot-img167765.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (AB289994)")
![Western blot - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (AB289994), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k9m-antibody-epr26429-113-bsa-and-azide-free-ab289994--western-blot-img169913.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (AB289994)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Dot | ICC/IF | IHC-P | Flow Cyt (Intra) | IP | ChIP | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Not recommended | Expected | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Synthetic peptide - Human, Transfected cell line - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide - Human, Transfected cell line - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide - Human, Transfected cell line - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Synthetic peptide - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide - Human, Transfected cell line - Human | Dilution info - | Notes - |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide - Human, Transfected cell line - Human | Dilution info - | Notes - |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Recommended products
Rabbit Recombinant Monoclonal H3 mutated K9 antibody. Carrier free. Suitable for WB, Dot, Flow Cyt (Intra) and reacts with Human, Synthetic peptide - Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26429-113
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab289994 is the carrier-free version of Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] ab289969.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry (Intracellular) - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (ab289994), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k9m-antibody-epr26429-113-bsa-and-azide-free-ab289994--flow-cytometry-intracellular-img171841.jpg "Flow Cytometry (Intracellular) - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (ab289994)")
Flow Cytometry (Intracellular) - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (ab289994)
This data was developed using Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] ab289969, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell line) cells transfected with a human Histone H3 WT expression vector containing a myc-His-tag® (Left) / HEK-293T cells transfected with a human Histone H3 (K9M) expression vector containing a myc-His-tag® (Right) labeling Histone H3 K9M mutant with Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] ab289969 at 1/5000 dilution. The secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution.
![Dot Blot - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (ab289994), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k9m-antibody-epr26429-113-bsa-and-azide-free-ab289994--dot-blot-img167765.jpg "Dot Blot - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (ab289994)")
Dot Blot - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (ab289994)
This data was developed using Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] ab289969, the same antibody clone in a different buffer formulation.
Dot blot analysis of Histone H3 K9M mutant using Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] ab289969 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution.
The samples are as follows:
Lane 1: Human Histone H3 (K9M) peptide (aa4-15)
Lane 2: Human Histone H3 (K9M) peptide (aa2-12)
Lane 3: Human Histone H3 WT peptide (aa2-15)
Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST
![Western blot - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (ab289994), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-mutated-k9m-antibody-epr26429-113-bsa-and-azide-free-ab289994--western-blot-img169913.jpg "Western blot - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (ab289994)")
Western blot - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] - BSA and Azide free (ab289994)
This data was developed using Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] ab289969, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The bands above 25 kDa are expected to be non-specific bands.
Exposure time: 37 seconds
All lanes: Western blot - Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] (Anti-Histone H3 (mutated K9M) antibody [EPR26429-113] ab289969) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) cells transfected with a human Histone H3 (K9M) expression vector containing a myc-His tag whole cell lysate at 20 µg
Lane 2: HEK-293T cells transfected with human Histone H3 WT expression vector containing a myc-His-tag whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 15 kDa
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