Rabbit Polyclonal H3 phospho S10 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF, IHC - Wmt and reacts with Human, Mouse, Rat, Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 phospho S10.
Images
Publications
Filter by
- Glia 71:259-2832022Pten associates with important gene regulatory network to fine-tune Müller glia-mediated zebrafish retina regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShivangi Gupta,Poonam Sharma,Mansi Chaudhary,Sharanya Premraj,Simran Kaur,Vijithkumar Vijayan,Manas Geeta Arun,Nagaraj Guru Prasad,Rajesh RamachandranPubMed 36128720
- Cell proliferation 55:e132882022Peripubertal requirement of Tsg101 in maintaining the integrity of membranous structures in mouse oocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyejin Shin,Dayoung Park,Jiyeon Kim,Min-Yeong Nam,Sojung Kwon,Da-Eun Um,Ji-Eun Oh,Esther Youn,Yhong-Hee Shim,Kay-Uwe Wagner,Jin Hyun Jun,Hye-Ryun Kim,Haengseok Song,Hyunjung Jade LimPubMed 35768997
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human H3C1 phospho S10. The exact immunogen used to generate this antibody is proprietary information. Database link P68431
Consider this alternative
Reactivity data
Select an application| IP | ChIP | WB | IHC-P | ICC/IF | IHC - Wmt | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested | Expected |
| Mouse | Expected | Expected | Tested | Expected | Expected | Expected |
| Rat | Expected | Expected | Tested | Expected | Expected | Expected |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Zebrafish | Expected | Expected | Expected | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IHC - Wmt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3S10p
Recommended products
Rabbit Polyclonal H3 phospho S10 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF, IHC - Wmt and reacts with Human, Mouse, Rat, Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 phospho S10.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human H3C1 phospho S10. The exact immunogen used to generate this antibody is proprietary information. Database link P68431
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
ChIP - Anti-Histone H3 (phospho S10) antibody (ab183626)
Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 μg of either control rabbit IgG or anti-Histone H3 (phospho S10) antibody (ab183626). The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.
Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626)
All lanes: Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/1000 dilution
Lane 1: U2OS whole cell lysate (untreated) at 30 µg
Lane 2: U2OS whole cell lysate (serum deprivation for 48hr) at 30 µg
Lane 3: U2OS whole cell lysate (100ng/ml Nocodazole treatment for 24hr) at 30 µg
Predicted band size: 15 kDa
Immunoprecipitation - Anti-Histone H3 (phospho S10) antibody (ab183626)
ab183626 immunoprecipitating Histone H3 in HeLa whole cell lysate.
Lane (-): Untreated HeLa whole cell lysate (30 μg)
Lane (+): Treated HeLa whole cell lysate (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ab183626 diluted at 1:2000.
For western blotting, HRP-conjugated anti-rabbit IgG was used as a secondary antibody.
All lanes: Immunoprecipitation - Anti-Histone H3 (phospho S10) antibody (ab183626)
Predicted band size: 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody (ab183626)
Confocal immunofluorescent analysis of mitotic HeLa cells labeling Histone H3 (phospho S10) with ab183626 at 1/500 (green). Red: Tubulin, alpha 1b protein stained by TUBA1B antibody at 1/2500 . Blue: Hoechst 33342 staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody (ab183626)
Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Histone H3 (phospho S10) with ab183626 at 1/500.
Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626)
All lanes: Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/500 dilution
Lane 1: Rat2 whole cell lysate at 30 µg
Lane 2: Rat2 whole cell lysate (100 ng/ml Nocodazole treatment for 24 hr) at 30 µg
Predicted band size: 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10) antibody (ab183626)
Immunofluorescent analysis of RFP-tag transfected HeLa cells labeling Histone H3 (phospho S10) with ab183626 at 1/500 (green). Red: Tubulin, alpha 1b protein stained by TUBA1B antibody at 1/500 . Blue: Hoechst 33342 staining.
Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626)
All lanes: Western blot - Anti-Histone H3 (phospho S10) antibody (ab183626) at 1/1000 dilution
Lane 1: 4T1 whole cell lysate at 30 µg
Lane 2: 4T1 whole cell lysate (100ng/ml Nocodazole treatment for 24hr) at 30 µg
Predicted band size: 15 kDa
IHC - Wholemount - Anti-Histone H3 (phospho S10) antibody (ab183626)
Immunohistochemistry (wholemount) analysis of zebrafish labelling Histone H3 (phopho S10) with ab183626 at a 1/100 dilution.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
IHC - Wholemount - Anti-Histone H3 (phospho S10) antibody (ab183626)
Immunohistochemistry (wholemount) analysis of zebrafish labelling Histone H3 (phopho S10) with ab183626 at a 1/100 dilution.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com