Rabbit Polyclonal H3 phospho S10 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 327 publications.
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- Frontiers in cell and developmental biology 11:12705422023Myt1 overexpression mediates resistance to cell cycle and DNA damage checkpoint kinase inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSargun Sokhi,Cody W Lewis,Amirali B Bukhari,Joanne Hadfield,Edric J Xiao,Jeremy Fung,Yea Jin Yoon,Wen-Hsin Hsu,Armin M Gamper,Gordon K ChanPubMed 38020882
- Nature communications 14:64432023STRA8-RB interaction is required for timely entry of meiosis in mouse female germ cells.Applications:ICC/IFReactive species:MouseRyuki Shimada,Yuzuru Kato,Naoki Takeda,Sayoko Fujimura,Kei-Ichiro Yasunaga,Shingo Usuki,Hitoshi Niwa,Kimi Araki,Kei-Ichiro IshiguroPubMed 37880249
- International journal of molecular sciences 24:2023Chemical Inhibition of RPA by HAMNO Alters Cell Cycle Dynamics by Impeding DNA Replication and G2-to-M Transition but Has Little Effect on the Radiation-Induced DNA Damage Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRositsa Dueva,Lisa Marie Krieger,Fanghua Li,Daxian Luo,Huaping Xiao,Martin Stuschke,Eric Metzen,George IliakisPubMed 37834389
- The Journal of cell biology 222:2023Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZihan Zhu,Isabelle Becam,Corinne A Tovey,Abir Elfarkouchi,Eugenie C Yen,Fred Bernard,Antoine Guichet,Paul T ConduitPubMed 37698931
- British journal of pharmacology 180:3271-32892023A novel small molecule inhibitor of p38⍺ MAP kinase augments cardiomyocyte cell cycle entry in response to direct cell cycle stimulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRiham R E Abouleisa,Jessica M Miller,Ahmad Gebreil,Abou Bakr M Salama,Marc Dwenger,Hania Abdelhafez,Reham M Wahid,Adeniyi T Adewumi,Mahmoud E S Soliman,Nader E Abo-Dya,Tamer M A MohamedPubMed 37547998
- Cells 12:2023Prox1 Suppresses the Proliferation of Breast Cancer Cells via Direct Inhibition of c-Myc Gene Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArtemis Michail,Dimitrios Gkikas,Dimitris Stellas,Valeria Kaltezioti,Panagiotis K PolitisPubMed 37508533
- International journal of molecular sciences 24:20237-Ketocholesterol Promotes Retinal Pigment Epithelium Senescence and Fibrosis of Choroidal Neovascularization via IQGAP1 Phosphorylation-Dependent Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaibo Wang,Aniket Ramshekar,Thaonhi Cung,Chris Wallace-Carrete,Chandler Zaugg,Jasmine Nguyen,Gregory J Stoddard,M Elizabeth HartnettPubMed 37373423
- Cell death and differentiation 30:1786-17982023TMEM11 regulates cardiomyocyte proliferation and cardiac repair via METTL1-mediated mG methylation of ATF5 mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin-Zhe Chen,Xin-Min Li,Shi-Jun Xu,Shen Hu,Tao Wang,Rui-Feng Li,Cui-Yun Liu,Jun-Qiang Xue,Lu-Yu Zhou,Yun-Hong Wang,Pei-Feng Li,Kun WangPubMed 37286744
- International journal of molecular sciences 24:2023An Evaluation of the Cytotoxic and Genotoxic Effects of the Marine Toxin C17-SAMT in Human TK6 and HepaRG Cell Lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeineb Marzougui,Ludovic Le Hegarat,Kevin Hogeveen,Sylvie Huet,Riadh Kharrat,Riadh Marrouchi,Valérie FessardPubMed 37175512
- Investigative ophthalmology & visual science 64:232023AURKB Enhances Chromosomal Remodeling of Telomeric Genes and Accelerates Tumorigenesis of Uveal Melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuixue Wang,Hui Pan,Xiaolin HuangPubMed 37079315
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ICC | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Mouse | Predicted | Predicted | Not recommended |
| Rat | Predicted | Predicted | Not recommended |
| Apteronotus leptorhynchus | Predicted | Predicted | Not recommended |
| Cyanidioschyzon merolae | Predicted | Predicted | Not recommended |
| Drosophila melanogaster | Predicted | Predicted | Not recommended |
| Hamster | Predicted | Predicted | Not recommended |
| Hydractinia echinata | Predicted | Predicted | Not recommended |
| Xenopus laevis | Predicted | Predicted | Not recommended |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Hamster, Xenopus laevis, Drosophila melanogaster, Apteronotus leptorhynchus, Cyanidioschyzon merolae, Hydractinia echinata | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Hamster, Xenopus laevis, Drosophila melanogaster, Apteronotus leptorhynchus, Cyanidioschyzon merolae, Hydractinia echinata | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Hamster, Xenopus laevis, Drosophila melanogaster, Apteronotus leptorhynchus, Cyanidioschyzon merolae, Hydractinia echinata | Dilution info - | Notes - |
Associated Products
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3S10p
Recommended products
Rabbit Polyclonal H3 phospho S10 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 327 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is specific for phosho S10 of histone H3. We believe that it does not recognise the non-modified histone - no blocking is seen with the non-phospho peptide (see Western blot image).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
This image is courtesy of a customer review submitted by Miss Anita CiurciuImmunocytochemistry - Anti-Histone H3 (phospho S10) antibody (ab5176)
ab5176 at a 1/100 dilution staining Drosophila melanogaster (wild type) polytene chromosomes by ICC/IF. The cells were formaldehyde fixed and blocked with 1% BSA prior to incubation with the antibody for 12 hours. Bound antibody was detected using a Cy3 conjugated goat anti-rabbit antibody.
Yamanaka S et al. Enhanced proliferation of monolayer cultures of embryonic stem (ES) cell-derived cardiomyocytes following acute loss of retinoblastoma. PLoS ONE 3:e3896 (2008).Immunocytochemistry - Anti-Histone H3 (phospho S10) antibody (ab5176)
Images of mouse cardiomyocyte in various stages of mitosis. Puromycin-resistant cells were stained with Hoechst 33342 (blue), and probed with antibodies specific to cTnT (Green) and Histone H3 (phospho S10) (Red) using ab5176 at 1/200 dilution in ICC/IF.
Mitotic activity was confirmed by staining with an antibody to phospho-H3 Histone, which is only phosphorylated at Ser10 during mitosis. Mitotic nuclei are pink (red+blue). Bar =10 μm.
Image courtesy of Dr. Bryan Turner, United KingdomImmunocytochemistry - Anti-Histone H3 (phospho S10) antibody (ab5176)
This image was kindly supplied by Prof Bryan Turner, University of Birmingham. Female Human Lymphoblastoid cells were incubated with ab5176.
Western blot - Anti-Histone H3 (phospho S10) antibody (ab5176)
Lysates:
Lane 1,3,5: 0.5µg/lane untreated histone
Lane 2,4,6: 0.5µg/lane colcemid treated histone - treated
Primary antibody:
Lane 1-2 : ab5176 (1g/ml)
Lane 3-4 :ab5176 (1g/ml) + phospho peptide (ab5176) 1g/ml
Lane 5-6 :ab5176 (1g/ml) + non-phospho peptide ( Human Histone H3 (unmodified ) peptide ab2903 batch 3) 1g/mlAll lanes: Western blot - Anti-Histone H3 (phospho S10) antibody (ab5176) at 1 µg/mL
Lanes 1, 3 and 5: untreated histones at 0.5 µg
Lanes 2, 4 and 6: colcemid treated histones at 0.5 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15-20 kDa
Exposure time: 10s
Western blot - Anti-Histone H3 (phospho S10) antibody (ab5176)
All lanes: Western blot - Anti-Histone H3 (phospho S10) antibody (ab5176) at 1/5000 dilution
Lane 1: Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2: HeLa cell lysate treated with calyculin A at 10 µg
Lane 3: HeLa cell lysate treated with calyculin A and alkaline phosphatase at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/20000 dilution
Predicted band size: 15 kDa
Exposure time: 1s
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