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AB32107

Anti-Histone H3 (phospho S10 + T11) antibody [E173]

5

(4 Reviews)

|

(67 Publications)

Anti-Histone H3 (phospho S10 + T11) antibody [E173] (ab32107) is a rabbit monoclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ELISA, Dot Blot. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications
- Trusted since 2006

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

9 Images
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
  • ICC/IF

AbReview6860****

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)

ab32107 (1/1000) staining Histone H3 (phospho S10 + T11) in paraformaldehyde-fixed, DAPI counterstained HeLa cells. Secondary antibody : Goat anti-Rabbit IgG conjugated to Cy3® (1/200). Please refer to abreview for further details.

This image is courtesy of an Abreview submitted by Kirk Mcmanus.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling Histone H3 (phospho S10 + T11) with ab32107 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor® 594). DAPI (blue) was used as a nuclear counterstain. Confocal image showing nuclear staining on mitotic HeLa cell.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)

ab32107, at a 1/50 dilution, staining Histone H3 (phospho S10 + T11) in paraffin embedded lymphoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
  • IP

Unknown

Immunoprecipitation - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)

Purified ab32107 at 1/50 dilution (2μg) immunoprecipitating Histone H3 in HeLa treated with calyculin A whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with calyculin A whole cell lysate 10μg
Lane 2 (+) : ab32107 + HeLa treated with calyculin A whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32107 in HeLa treated with calyculin A whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 17 kDa

All lanes:

Immunoprecipitation - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (ab32107)

Predicted band size: 15 kDa

false

Western blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
  • WB

Lab

Western blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)

Blocking/Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (ab32107) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate -treated with Calyculin A with Histone H3 phospho S10 peptide

Lane 2:

HeLa whole cell lysate -treated with Calyculin A with Histone H3 phospho T11 peptide

Lane 3:

HeLa whole cell lysate -treated with Calyculin A with unmodified Histone H3 peptide

Lane 4:

HeLa whole cell lysate -treated with Calyculin A with no peptide

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 1s

Western blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
  • WB

Lab

Western blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (ab32107) at 1/10000 dilution

Lane 1:

Untreated HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with calyculin A at 10 µg

Lane 3:

HeLa cell lysate treated with calyculin A and alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 1s

ELISA - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
  • ELISA

Unknown

ELISA - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)

Direct ELISA antibody dose-response curve using ab32107 (0-1000 ng/mL).

Peptides - Histone H3 unmodified peptide, Histone H3 phospho S10 peptide, Histone H3 phospho T11 peptide (10 ng/mL).

Secondary antibody - Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (1/2500).

ELISA - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
  • ELISA

Unknown

ELISA - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)

Direct ELISA antibody dose-response curve using ab32107 (0-1000 ng/mL).

Peptides - Histone H3 unmodified peptide, Histone H3 phospho S10 peptide, Histone H3 phospho T11 peptide (100 ng/mL).

Secondary antibody - Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (1/2500).

Dot Blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)

Dot blot analysis of Histone H3 phospho S10 peptide (Lane 1), Histone H3 phospho T11 peptide (Lane 2) and Histone H3 unmodified peptide (Lane 3) labelling Histone H3 (phospho S10 + T11) with ab32107 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) was used as the secondary antibody at a dilution of 1/10 000.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Histone H3 (phospho S10 + T11) antibody [E173]

  • HRP

    HRP Anti-Histone H3 (phospho S10 + T11) antibody [E173]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Histone H3 (phospho S10 + T11) antibody [E173]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Histone H3 (phospho S10 + T11) antibody [E173]

  • Carrier free

    Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E173

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Dot, ICC/IF, WB, ELISA, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects Histone H3 phosphorylated on both Serine 10 and Threonine 11. However, the antibody shows higher affinity for phosphorylated Serine 10 than for phosphorylated Threonine 11. This was validated by ELISA, Dot Blot and WB peptide blocking experiments.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/250 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-Histone H3 (phospho S10 + T11) antibody [E173] (ab32107) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Dot Blot in Human, Mouse samples.

What is the molecular weight of Histone H3?
Anti-Histone H3 (phospho S10 + T11) [E173] (ab32107) specifically detects a band for Histone H3 (UniProt: Q16695) at a molecular weight of 15kDa.

Trusted by the scientific community
Anti-Histone H3 (phospho S10 + T11) [E173] (ab32107) was first used in a scientific publication in 2006 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [E173] also available for your convenience: ab32107, Alexa Fluor® 647 - ab200523, Alexa Fluor® 488 - ab200614, HRP - ab200616, Carrier free - ab239803

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 phospho S10 + T11
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Publications (67)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiovascular development and disease 12: PubMed40710803

2025

Nuclear Lactate Dehydrogenase A Resists Cardiomyocyte Cell Cycle Arrest Induced by Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Mengfei Cao,Jie Luo,Kewei Fu,Yao Xu,Yinyu Wang,Junying Duan,Rui Chen,Wei Yuan

Nature communications 16:1232 PubMed39890822

2025

Regeneration in the absence of canonical neoblasts in an early branching flatworm.

Applications

Unspecified application

Species

Unspecified reactive species

Ludwik Gąsiorowski,Chew Chai,Andrei Rozanski,Gargi Purandare,Fruzsina Ficze,Athanasia Mizi,Bo Wang,Jochen C Rink

Nature communications 15:10681 PubMed39672898

2024

Mitochondrial dynamics govern whole-body regeneration through stem cell pluripotency and mitonuclear balance.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Pan,Yun Zhao,Yucong Li,Jiajia Chen,Wenya Zhang,Ling Yang,Yuanyi Zhou Xiong,Yuqing Ying,Hao Xu,Yuhong Zhang,Chong Gao,Yuhan Sun,Nan Li,Liangyi Chen,Zhixing Chen,Kai Lei

PloS one 19:e0309471 PubMed39570878

2024

Immunohistochemical study of histone protein 3 modification in pediatric osteosarcoma identifies reduced H3K27me3 as a marker of poor treatment response.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Kondratowski,Danielle Cohen,Rebecca J Deyell,Ash Sandhu,Jonathan W Bush

The EMBO journal 43:6591-6625 PubMed39567829

2024

Fibrillarin homologs regulate translation in divergent cell lineages during planarian homeostasis and regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Chen,Yucong Li,Yan Wang,Hui Wang,Jiaqi Yang,Xue Pan,Yun Zhao,Hao Xu,Penglei Jiang,Pengxu Qian,Hongwei Wang,Zhi Xie,Kai Lei

International journal of molecular sciences 25: PubMed39273571

2024

Protein Kinase C-Delta Mediates Cell Cycle Reentry and Apoptosis Induced by Amyloid-Beta Peptide in Post-Mitotic Cortical Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Hsuan Wu,A-Ching Chao,Yi-Heng Hsieh,You Lien,Yi-Chun Lin,Ding-I Yang

Nature communications 15:6480 PubMed39090108

2024

Tregs delivered post-myocardial infarction adopt an injury-specific phenotype promoting cardiac repair via macrophages in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmin K Alshoubaki,Bhavana Nayer,Yen-Zhen Lu,Ekaterina Salimova,Sin Nee Lau,Jean L Tan,Daniela Amann-Zalcenstein,Peter F Hickey,Gonzalo Del Monte-Nieto,Ajithkumar Vasanthakumar,Mikaël M Martino

International journal of molecular sciences 25: PubMed39000427

2024

Polysaccharides from Protect Post-Mitotic Neurons against Cell Cycle Re-Entry and Apoptosis Induced by the Amyloid-Beta Peptide by Blocking Sonic Hedgehog Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Yu Hou,Ming-Hsuan Wu,Hui-Yu Hsu,Yi-Chun Lin,Ding-I Yang

Theranostics 14:1371-1389 PubMed38389850

2024

Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Gao,Tuo Zhang,Tengxiang Chen,Ziqi Chen,Zijian Zhu,Yang Wen,Shaogang Qin,Yibing Bao,Ting Zhao,Hengxing Li,Longping Liu,Ming Hao,Jianbin Wang,Fengchao Wang,Haibin Wang,Bo Zhou,Hua Zhang,Guoliang Xia,Chao Wang

Frontiers in oncology 13:1279933 PubMed38023177

2023

A prognostic model for tumor recurrence and progression after meningioma surgery: preselection for further molecular work-up.

Applications

Unspecified application

Species

Unspecified reactive species

Luis Padevit,Flavio Vasella,Jason Friedman,Valentino Mutschler,Freya Jenkins,Ulrike Held,Elisabeth Jane Rushing,Hans-Georg Wirsching,Michael Weller,Luca Regli,Marian Christoph Neidert
View all publications
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