Anti-Histone H3 (phospho S10 + T11) antibody [E173]
- 20ul selling size
- RabMAb
- Recombinant
- What is this?
5
(4 Reviews)
|
(67 Publications )
- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications
- Trusted since 2006
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
ab32107, at a 1/50 dilution, staining Histone H3 (phospho S10 + T11) in paraffin embedded lymphoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling Histone H3 (phospho S10 + T11) with ab32107 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor® 594). DAPI (blue) was used as a nuclear counterstain. Confocal image showing nuclear staining on mitotic HeLa cell.
- ICC/IF
AbReview6860****
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
ab32107 (1/1000) staining Histone H3 (phospho S10 + T11) in paraformaldehyde-fixed, DAPI counterstained HeLa cells. Secondary antibody : Goat anti-Rabbit IgG conjugated to Cy3® (1/200). Please refer to abreview for further details.
This image is courtesy of an Abreview submitted by Kirk Mcmanus.
- IP
Unknown
Immunoprecipitation - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
Purified ab32107 at 1/50 dilution (2μg) immunoprecipitating Histone H3 in HeLa treated with calyculin A whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with calyculin A whole cell lysate 10μg
Lane 2 (+) : ab32107 + HeLa treated with calyculin A whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32107 in HeLa treated with calyculin A whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 17 kDa
All lanes:
Immunoprecipitation - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (ab32107)
Predicted band size: 15 kDa
false
- WB
Lab
Western blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (ab32107) at 1/10000 dilution
Lane 1:
Untreated HeLa cell lysate at 10 µg
Lane 2:
HeLa cell lysate treated with calyculin A at 10 µg
Lane 3:
HeLa cell lysate treated with calyculin A and alkaline phosphatase at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa
false
Exposure time: 1s
- WB
Lab
Western blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
Blocking/Diluting buffer 5% NFDM/TBST
All lanes:
Western blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (ab32107) at 1/1000 dilution
Lane 1:
HeLa whole cell lysate -treated with Calyculin A with Histone H3 phospho S10 peptide
Lane 2:
HeLa whole cell lysate -treated with Calyculin A with Histone H3 phospho T11 peptide
Lane 3:
HeLa whole cell lysate -treated with Calyculin A with unmodified Histone H3 peptide
Lane 4:
HeLa whole cell lysate -treated with Calyculin A with no peptide
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
false
Exposure time: 1s
- ELISA
Unknown
ELISA - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
Direct ELISA antibody dose-response curve using ab32107 (0-1000 ng/mL).
Peptides - Histone H3 unmodified peptide, Histone H3 phospho S10 peptide, Histone H3 phospho T11 peptide (10 ng/mL).
Secondary antibody - Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (1/2500).
- ELISA
Unknown
ELISA - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
Direct ELISA antibody dose-response curve using ab32107 (0-1000 ng/mL).
Peptides - Histone H3 unmodified peptide, Histone H3 phospho S10 peptide, Histone H3 phospho T11 peptide (100 ng/mL).
Secondary antibody - Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (1/2500).
- Dot
Unknown
Dot Blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (AB32107)
Dot blot analysis of Histone H3 phospho S10 peptide (Lane 1), Histone H3 phospho T11 peptide (Lane 2) and Histone H3 unmodified peptide (Lane 3) labelling Histone H3 (phospho S10 + T11) with ab32107 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) was used as the secondary antibody at a dilution of 1/10 000.
Blocking and diluting buffer : 5% NFDM/TBST.
Exposure time : 3 minutes.
Related conjugates and formulations (5)
-
Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Histone H3 (phospho S10 + T11) antibody [E173]
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Histone H3 (phospho S10 + T11) antibody [E173]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Histone H3 (phospho S10 + T11) antibody [E173]
-
HRP Anti-Histone H3 (phospho S10 + T11) antibody [E173]
Reactivity data
Product details
What is this antibody validated in?
Anti-Histone H3 (phospho S10 + T11) antibody [E173] (ab32107) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Dot Blot in Human, Mouse samples.
What is the molecular weight of Histone H3?
Anti-Histone H3 (phospho S10 + T11) [E173] (ab32107) specifically detects a band for Histone H3 (UniProt: Q16695) at a molecular weight of 15kDa.
Trusted by the scientific community
Anti-Histone H3 (phospho S10 + T11) [E173] (ab32107) was first used in a scientific publication in 2006 and has been cited over 50 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [E173] also available for your convenience: ab32107, Alexa Fluor® 647 - ab200523, Alexa Fluor® 488 - ab200614, HRP - ab200616, Carrier free - ab239803
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Alternative Names
Publications (67)
Recent publications for all applications. Explore the full listand refine your search
Journal of cardiovascular development and disease 12: PubMed40710803
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 16:1232 PubMed39890822
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 15:10681 PubMed39672898
2024
Applications
Unspecified application
Species
Unspecified reactive species
PloS one 19:e0309471 PubMed39570878
2024
Applications
Unspecified application
Species
Unspecified reactive species
The EMBO journal 43:6591-6625 PubMed39567829
2024
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 25: PubMed39273571
2024
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 15:6480 PubMed39090108
2024
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 25: PubMed39000427
2024
Applications
Unspecified application
Species
Unspecified reactive species
Theranostics 14:1371-1389 PubMed38389850
2024
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in oncology 13:1279933 PubMed38023177
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com