Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (AB239803)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling Histone H3 (phospho S10 + T11) with ab32107 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor^® 594). DAPI (blue) was used as a nuclear counterstain. Confocal image showing nuclear staining on mitotic HeLa cell.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (AB239803)

ab32107, at a 1/50 dilution, staining Histone H3 (phospho S10 + T11) in paraffin embedded human lymphoma tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

AbReview6860****

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (AB239803)

ab32107 (1/1000) staining Histone H3 (phospho S10 + T11) in paraformaldehyde-fixed, DAPI counterstained HeLa cells. Secondary antibody : Goat anti-Rabbit IgG conjugated to Cy3® (1/200). Please refer to abreview for further details.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).

This image is courtesy of an Abreview submitted by Kirk Mcmanus.

• IP

Unknown

Immunoprecipitation - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (AB239803)

Purified ab32107 at 1/50 dilution (2μg) immunoprecipitating Histone H3 in HeLa treated with calyculin A whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with calyculin A whole cell lysate 10μg
Lane 2 (+) : ab32107 + HeLa treated with calyculin A whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32107 in HeLa treated with calyculin A whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 17 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).

All lanes:

Immunoprecipitation - Anti-Histone H3 (phospho S10 + T11) antibody [E173] (<a href='/en-us/products/primary-antibodies/histone-h3-phospho-s10-t11-antibody-e173-ab32107'>ab32107</a>)

Predicted band size: 15 kDa

false

• ELISA

Unknown

ELISA - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (AB239803)

Direct ELISA antibody dose-response curve using ab32107 (0-1000 ng/mL).

Peptides - Histone H3 unmodified peptide, Histone H3 phospho S10 peptide, Histone H3 phospho T11 peptide (10 ng/mL).

Secondary antibody - Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (1/2500).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).

• ELISA

Unknown

ELISA - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (AB239803)

Direct ELISA antibody dose-response curve using ab32107 (0-1000 ng/mL).

Peptides - Histone H3 unmodified peptide, Histone H3 phospho S10 peptide, Histone H3 phospho T11 peptide (100 ng/mL).

Secondary antibody - Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (1/2500).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).

• Dot

Unknown

Dot Blot - Anti-Histone H3 (phospho S10 + T11) antibody [E173] - BSA and Azide free (AB239803)

Dot blot analysis of Histone H3 phospho S10 peptide (Lane 1), Histone H3 phospho T11 peptide (Lane 2) and Histone H3 unmodified peptide (Lane 3) labelling Histone H3 (phospho S10 + T11) with ab32107 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) was used as the secondary antibody at a dilution of 1/10 000.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32107).