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Rabbit Polyclonal H3 phospho S28 antibody. Suitable for WB, PepArr, ICC/IF and reacts with Human, Synthetic peptide - Human samples. Cited in 17 publications.


Images

Western blot - Anti-Histone H3 (phospho S28) antibody (AB5169), expandable thumbnail
  • Western blot - Anti-Histone H3 (phospho S28) antibody (AB5169), expandable thumbnail
  • Peptide Array - Anti-Histone H3 (phospho S28) antibody (AB5169), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S28) antibody (AB5169), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S28) antibody (AB5169), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBPepArrICC/IF
Human
Tested
Expected
Tested
Drosophila melanogaster
Predicted
Predicted
Predicted
Synthetic peptide - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Drosophila melanogaster

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide - Human

Dilution info

0.00200-0.02 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Drosophila melanogaster

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

-

Predicted
Predicted

Species

Drosophila melanogaster

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

Recommended products

Rabbit Polyclonal H3 phospho S28 antibody. Suitable for WB, PepArr, ICC/IF and reacts with Human, Synthetic peptide - Human samples. Cited in 17 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Specificity

This antibody is specific for Histone H3 phosphorylated at residue Ser 28 and does not recognise the unmodified residue or another phosphorylated residue (Ser 10) on the same histone.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Histone H3 (phospho S28) antibody (ab5169), expandable thumbnail
    Image courtesy of Richelle Sopko, Harvard University, U.S.A.

    Western blot - Anti-Histone H3 (phospho S28) antibody (ab5169)

    0-4hr old wee shRNA embryos (lane 2) should display elevated phH3Ser28 levels relative to 0-4hr old EGFP shRNA embryos (lane 1). Blocked with 10% BSA.

    All lanes: Western blot - Anti-Histone H3 (phospho S28) antibody (ab5169) at 1/1000 dilution

    Lane 1: Wild type 0-4 hour old fruit fly embryos.

    Lane 2: 0-4 hour old fruit fly embryos expressing wee RNAi

    Secondary

    All lanes: Donkey anti-rabbit IgG, Horseradish Peroxidase-L at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa

    Exposure time: 10min

  • Western blot - Anti-Histone H3 (phospho S28) antibody (ab5169), expandable thumbnail

    Western blot - Anti-Histone H3 (phospho S28) antibody (ab5169)

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab5169 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-Histone H3 (phospho S28) antibody (ab5169) at 1 µg/mL

    All lanes: Hela Whole Cell Lysate - Colcemid Treated at 2.5 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa

    Observed band size: 17 kDa

    Exposure time: 30s

  • Peptide Array - Anti-Histone H3 (phospho S28) antibody (ab5169), expandable thumbnail

    Peptide Array - Anti-Histone H3 (phospho S28) antibody (ab5169)

    All batches of ab5169 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - phospho S28 peptide (ab5499), indicating that this antibody specifically recognises the Histone H3 - phospho S28 modification.

    ab17163 - Histone H3 unmodified

    ab5499 - Histone H3 - phospho S28

    Human Histone H3 (phospho S10) peptide ab11477 - Histone H3 - phospho S10

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S28) antibody (ab5169), expandable thumbnail
    The image was submitted as part of a review by Krik McManus, University of British Columbia

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S28) antibody (ab5169)

    Indian Muntjac (top panel) and HeLa cells (bottom panel) immunofluorescently labelled with ab5169 (green) at a working dilution of 1/5000. The DNA is counterstained with DAPI and is shown in blue in the top panel and red in the bottom panel. This antibody gives a characteristic staining pattern for Histone H3 (phospho S28) whereby the signal increases in intensity during late G2 and continues to increase until metaphase. Upon entry into anaphase the signal begins to decrease until reaching basal levels by early G1. 100x magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S28) antibody (ab5169), expandable thumbnail
    This image was submitted as part of a review by Kirk McManus, University of British Columbia

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S28) antibody (ab5169)

    In situ peptide competition was perfomed on paraformaldehyde-fixed HeLa cells. Four 25μl aliquots were made, to which 7.5μg (1.5μl) of no peptide, H3 unmodified peptide (Human Histone H3 (unmodified ) peptide ab2623), H3 phospho S10 peptide (Human Histone H3 (phospho S10) peptide ab11477) or H3 phospho S28 peptide (ab5499) was added, mixed by vortexing and incubated for 1 hour at room temperature. Cells on glass coverslips were fixed with 4% paraformaldehyde (10min) and gently washed twice with PBS, then permeabilized with 0.5% Triton X-100 in PBS (10min) and gently washed three times with PBS. The cells were immunofluorescently labeled with either the peptide-competed antibody or the control antibody (i.e. no peptide) for 30min at room temperature, washed briefly with PBS containing 0.1% Triton X-100 (1 min) and twice with PBS. The cells were then incubated with an appropriate dilution of a secondary antibody at room temperature for 30min, rinsed as above and mounted using a 90% glycerol in PBS mount

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