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Rabbit Recombinant Monoclonal H3 phospho S10 antibody. Carrier free. Suitable for IHC-P, IHC - Wmt, ICC/IF, WB and reacts with Mouse, Zebrafish, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C1 phospho S10.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373), expandable thumbnail
  • Western blot - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human H3C1 phospho S10. The exact immunogen used to generate this antibody is proprietary information. Database link P68431

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIHC - WmtICC/IFWB
Human
Expected
Expected
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Zebrafish
Expected
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Zebrafish, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Zebrafish
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse, Zebrafish
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Zebrafish
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, H3C1, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3S10p

Recommended products

Rabbit Recombinant Monoclonal H3 phospho S10 antibody. Carrier free. Suitable for IHC-P, IHC - Wmt, ICC/IF, WB and reacts with Mouse, Zebrafish, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C1 phospho S10.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human H3C1 phospho S10. The exact immunogen used to generate this antibody is proprietary information. Database link P68431
Carrier free
Yes
Clone number
HL1752
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (ab308373), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (ab308373)

    Immunohistochemical analysis of Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo tissue labeling Histone H3S10ph phospho Ser10 (green) using ab308373 at 1/100 dilution. Antigen retrival was performed using Tris-HCl buffer, pH 9.0, 20 min at 70C

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (ab308373), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (ab308373)

    Immunohistochemical analysis of mouse intestine tissue labeling Histone H3S10ph phospho Ser10 using ab308373 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Western blot - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (ab308373), expandable thumbnail

    Western blot - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (ab308373)

    Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody [HL1752] (ab308373) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    Lane 1: Untreated (–) HeLa whole cell extracts at 30 µg

    Lane 2: Treated (+) HeLa whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (ab308373), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (ab308373)

    For immunofluorescence analysis, HeLa cells were fixed in 2% paraformaldehyde at 37C for 30 minutes and permeabilized for detection of Histone H3S10ph phospho Ser10 using ab308373 at 1/500 dilution (green). Red: alpha Tubulin, a cytoskeleton marker. Blue: Fluoroshield with DAPI.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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