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AB308373

Anti-Histone H3 (phospho Ser10) antibody [HL1752]

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(4 Publications)

Rabbit Recombinant Monoclonal H3 phospho S10 antibody. Carrier free. Suitable for IHC-P, IHC - Wmt, ICC/IF, WB and reacts with Mouse, Zebrafish, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human H3C1 phospho S10.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3S10p

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373)

For immunofluorescence analysis, HeLa cells were fixed in 2% paraformaldehyde at 37C for 30 minutes and permeabilized for detection of Histone H3S10ph phospho Ser10 using ab308373 at 1/500 dilution (green). Red : alpha Tubulin, a cytoskeleton marker. Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373)

Immunohistochemical analysis of mouse intestine tissue labeling Histone H3S10ph phospho Ser10 using ab308373 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373)
  • WB

Supplier Data

Western blot - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373)

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody [HL1752] (ab308373) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Untreated (–) HeLa whole cell extracts at 30 µg

Lane 2:

Treated (+) HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho Ser10) antibody [HL1752] (AB308373)

Immunohistochemical analysis of Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo tissue labeling Histone H3S10ph phospho Ser10 (green) using ab308373 at 1/100 dilution. Antigen retrival was performed using Tris-HCl buffer, pH 9.0, 20 min at 70C

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1752

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Zebrafish

Applications

ICC/IF, IHC-P, IHC - Wmt, WB

applications

Immunogen

Synthetic Peptide within Human H3C1 phospho S10. The exact immunogen used to generate this antibody is proprietary information.

P68431

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100", "IHCWmt-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 phospho S10
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:111255 PubMed39620128

2024

activates SLFN4MDSCs to accelerate gastric intestinal metaplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihong Zhao,Rui Liu,Ye Peng,Cun Li,Qian Li

Nucleic acids research 52:9886-9903 PubMed38943343

2024

Phosphorylation of the nuclear poly(A) binding protein (PABPN1) during mitosis protects mRNA from hyperadenylation and maintains transcriptome dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Jackson M Gordon,David V Phizicky,Leonard Schärfen,Courtney L Brown,Dahyana Arias Escayola,Jean Kanyo,TuKiet T Lam,Matthew D Simon,Karla M Neugebauer

Scientific reports 14:12728 PubMed38830934

2024

SPA inhibits hBMSC osteogenic differentiation and M1 macrophage polarization by suppressing SETD2 in acute suppurative osteomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Zhu,Feng Chen,Hongjia Qiang,Han Qi

International journal of molecular sciences 24: PubMed37762705

2023

Knockdown of BAP31 Downregulates Galectin-3 to Inhibit the Wnt/β-Catenin Signaling Pathway to Modulate 5-FU Chemosensitivity and Cancer Stemness in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Liu,Qi Zhang,Jiyu Wang,Changli Wang,Tian Lan,Tianyi Wang,Bing Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com