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AB272139

Anti-Histone H3 (phospho T3, di methyl K4) antibody

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(1 Publication)

Rabbit Polyclonal H3 phospho T3 + di methyl K4 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Mouse, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C15.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

4 Images
Western blot - Anti-Histone H3 (phospho T3, di methyl K4) antibody (AB272139)
  • WB

Unknown

Western blot - Anti-Histone H3 (phospho T3, di methyl K4) antibody (AB272139)

Block : 5% BLOTTO

All lanes:

Western blot - Anti-Histone H3 (phospho T3, di methyl K4) antibody (ab272139) at 1/50 dilution

All lanes:

NIH-3T3 histone prep lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3, di methyl K4) antibody (AB272139)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3, di methyl K4) antibody (AB272139)

Immunofluorescence analysis of HeLa cells labeling Histone H3 (green) using ab272139 at 1 : 50, followed by Dylight 488 secondary antibody at 1 : 10,000. Localization : Histone H3 [p Thr3, Monomethyl Lys4] is nuclear and chromosomal. Countersatin : nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).

ChIP - Anti-Histone H3 (phospho T3, di methyl K4) antibody (AB272139)
  • ChIP

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ChIP - Anti-Histone H3 (phospho T3, di methyl K4) antibody (AB272139)

Chromatin Immunoprecipitation of ab272139. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of ab272139 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Dot Blot - Anti-Histone H3 (phospho T3, di methyl K4) antibody (AB272139)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (phospho T3, di methyl K4) antibody (AB272139)

Dot Blot analysis of Histone H3 methylated forms using ab272137 at 1 : 1000, followed by Dylight 488 secondary antibody at 1 : 10,000. Load : 1, 10, and 100 picomoles of sample. Block : 5% BLOTTO.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, ChIP, Dot

applications

Immunogen

Synthetic Peptide within Human H3C15. The exact immunogen used to generate this antibody is proprietary information.

Q71DI3

Specificity

This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Anti-Histone H3 [Dimethyl Lys4, p Thr3] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 phospho T3 + di methyl K4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Protein science : a publication of the Protein Society 33:e5126 PubMed39073155

2024

Haspin mediates H3.3S31 phosphorylation downstream of Aurora B in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Li,Meixian Wu,Yang Liu,Lihua Sun,Peiqiang Mu,Binbin Ma,Jing Xie
View all publications
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