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AB272138

Anti-Histone H3 (phospho T3, mono methyl K4) antibody

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Rabbit Polyclonal H3 mono methyl K4 + phospho T3 antibody. Suitable for Dot, WB, ICC/IF and reacts with Synthetic peptide, Human, Mouse, Caenorhabditis elegans samples. Immunogen corresponding to Synthetic Peptide within Human H3C15.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3, mono methyl K4) antibody (AB272138)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3, mono methyl K4) antibody (AB272138)

Immunofluorescence analysis of 0.5% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Anti-Histone H3 (phospho T3, mono methyl K4) (green) with ab272138 at 1/100 dilution, followed by Dylight 488 secondary antibody at 1/10000 dilution. Counterstain : Nuclei and aplpha-tubulin were co-stained with DAPI (blue) and Dylight 550 (red).

Western blot - Anti-Histone H3 (phospho T3, mono methyl K4) antibody (AB272138)
  • WB

Unknown

Western blot - Anti-Histone H3 (phospho T3, mono methyl K4) antibody (AB272138)

All lanes:

Western blot - Anti-Histone H3 (phospho T3, mono methyl K4) antibody (ab272138) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) histone prep at 30 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) histone prep at 30 µg

Lane 3:

C.elegans embryo lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

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Dot Blot - Anti-Histone H3 (phospho T3, mono methyl K4) antibody (AB272138)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (phospho T3, mono methyl K4) antibody (AB272138)

Dot Blot analysis of modified forms of the Histone H3 immunizing peptide using ab272138 at 1/1000 dilution, followed by HRP rabbit secondary antibody at 1/40000 dilution. Load : 1, 10, and 100 picomoles of sample.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Caenorhabditis elegans

Applications

WB, Dot, ICC/IF

applications

Immunogen

Synthetic Peptide within Human H3C15. The exact immunogen used to generate this antibody is proprietary information.

Q71DI3

Specificity

This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Anti-Histone H3 [p Thr3, Monomethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 mono methyl K4 + phospho T3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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