Rabbit Polyclonal H3 phospho T6 antibody. Suitable for ICC/IF, WB, PepArr and reacts with Human, Mouse samples. Cited in 9 publications.
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- Nucleic acids research 47:10086-101032019The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJimsheena V Karakkat,Suneesh Kaimala,Sreejisha P Sreedharan,Princy Jayaprakash,Ernest A Adeghate,Suraiya A Ansari,Ernesto Guccione,Eric P K Mensah-Brown,Bright Starling EmeraldPubMed 31529049
- The Prostate 77:1452-14672017The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Song Yang et. al.PubMed 28875501
- Nucleic acids research 44:6102-122016Chromatin condensation and recruitment of PHD finger proteins to histone H3K4me3 are mutually exclusive.
- Molecular cell 59:502-112015An Interactive Database for the Assessment of Histone Antibody Specificity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesScott B Rothbart et. al.PubMed 26212453
- Theranostics 5:12-222015Histone H3 phosphorylation in GBM: a new rational to guide the use of kinase inhibitors in anti-GBM therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRomain Pacaud et. al.PubMed 25553095
- Nature 464:792-62010Phosphorylation of histone H3T6 by PKCbeta(I) controls demethylation at histone H3K4.
- Nature chemical biology 6:283-902010Combinatorial profiling of chromatin binding modules reveals multisite discrimination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam L Garske et. al.PubMed 20190764
- The EMBO journal 28:1878-892009Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and H3K56Ac in human cells.
- The Journal of biological chemistry 284:16575-832009Phosphorylation of histone H3 Thr-45 is linked to apoptosis.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | PepArr | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
PepArr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.02000-0.20000 µg/mL | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3T6p
Recommended products
Rabbit Polyclonal H3 phospho T6 antibody. Suitable for ICC/IF, WB, PepArr and reacts with Human, Mouse samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T6) antibody (ab14102)
ab14102 staining Histone H3 (phosphorylation T6) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab14102 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Western blot - Anti-Histone H3 (phospho T6) antibody (ab14102)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Histone H3 (phospho T6) antibody (ab14102) at 1 µg
All lanes: HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 8min
Peptide Array - Anti-Histone H3 (phospho T6) antibody (ab14102)
All batches of ab14102 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - phospho T6 peptide (ab14103), indicating that this antibody specifically recognises the Histone H3 - phospho T6 modification.
Human Histone H3 (unmodified) peptide ab7228 - Histone H3 unmodified
ab17578 - Histone H3 - phospho T3
ab14103 - Histone H3 - phospho T6
ab24444 - Histone H3 - phospho T11
Human Histone H3 (phospho S10) peptide ab11477 - Histone H3 - phospho S10
Human Histone H3 (citrulline R2 + R8 + R17) peptide ab32876 - Histone H3 - citrulline 2 + 8 + 17
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