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AB272145

Anti-Histone H3 (phospho T6, mono methyl K9) antibody

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(1 Publication)

Rabbit Polyclonal H3 phospho T6 + mono methyl K9 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Mouse, Caenorhabditis elegans, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C15.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)

Immunofluorescent analysis of 0.5% PFA-fixed HeLa cells labeling Histone H3 (phospho T6, mono methyl K9) using ab272145 at 1/50 dilution for 1 hour at room temperature (Green). FITC secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue).

ChIP - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)
  • ChIP

Unknown

ChIP - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)

ChIP analysis of chromatin from one million formaldehyde cross-linked HeLa cells using 2μg of ab272145 and 20μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)
  • WB

Unknown

Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)

Primary incubation overnight at 4°C.

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

All lanes:

Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145) at 1/1000 dilution

All lanes:

HeLa Histone preparation at 30 µg

Predicted band size: 15 kDa

false

Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)
  • WB

Unknown

Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)

Primary incubation overnight at 4°C.

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

All lanes:

Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145) at 1/1000 dilution

All lanes:

NIH/3T3 Histone preparation at 30 µg

Predicted band size: 15 kDa

false

Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)
  • WB

Unknown

Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)

Primary incubation overnight at 4°C.

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

Other band(s) : None.

All lanes:

Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145) at 1/1000 dilution

All lanes:

C. elegans embryo lysate at 30 µg

Predicted band size: 15 kDa

false

Dot Blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (AB272145)

Dot Blot analysis of Histone H3 (phospho T6, mono methyl K9) using ab272145 at 1/1000 dilution.

Lane 1 : Histone H3 1-13.

Lane 2 : T6p.

Lane 3 : T6pK9Me1.

Lane 4 : T6pK9Me2.

Lane 5 : T6pK9Me3.

Lane 6 : Histone H3 2-17.

Lane 7 : K9Me1.

Lane 8 : K9Me2.

Lane 9 : K9Me3.

Lane 10 : K9Ac.

Load : 1, 10, and 100 picomoles of peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Caenorhabditis elegans

Applications

ChIP, Dot, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human H3C15. The exact immunogen used to generate this antibody is proprietary information.

Q71DI3

Specificity

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Anti-Histone H3 [p Thr6, Monomethyl Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 phospho T6 + mono methyl K9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed36672612

2022

L-Serine Influences Epigenetic Modifications to Improve Cognition and Behaviors in Growth Hormone-Releasing Hormone Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Zhang,Mert Icyuz,Trygve Tollefsbol,Paul Alan Cox,Sandra Anne Banack,Liou Y Sun
View all publications
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