Rabbit Polyclonal H3 phospho T6 + mono methyl K9 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Mouse, Caenorhabditis elegans, Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Human H3C15.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human H3C15. The exact immunogen used to generate this antibody is proprietary information. Database link Q71DI3
Reactivity data
Select an application| ChIP | Dot | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
| Caenorhabditis elegans | Expected | Expected | Tested | Expected |
| Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-5 µg/cells for 6 µg/cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Caenorhabditis elegans | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Caenorhabditis elegans | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/1000 | Notes - |
Species Caenorhabditis elegans | Dilution info 1/500 - 1/1000 | Notes - |
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Caenorhabditis elegans | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Recommended products
Rabbit Polyclonal H3 phospho T6 + mono methyl K9 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Mouse, Caenorhabditis elegans, Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Human H3C15.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human H3C15. The exact immunogen used to generate this antibody is proprietary information. Database link Q71DI3
- Purification technique
- Affinity purification
- Specificity
A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
- Concentration
- Purification notes
Anti-Histone H3 [p Thr6, Monomethyl Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)
Primary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
All lanes: Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145) at 1/1000 dilution
All lanes: HeLa Histone preparation at 30 µg
Predicted band size: 15 kDa
ChIP - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)
ChIP analysis of chromatin from one million formaldehyde cross-linked HeLa cells using 2μg of ab272145 and 20μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)
Immunofluorescent analysis of 0.5% PFA-fixed HeLa cells labeling Histone H3 (phospho T6, mono methyl K9) using ab272145 at 1/50 dilution for 1 hour at room temperature (Green). FITC secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue).
Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)
Primary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
All lanes: Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145) at 1/1000 dilution
All lanes: NIH/3T3 Histone preparation at 30 µg
Predicted band size: 15 kDa
Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)
Primary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
Other band(s): None.
All lanes: Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145) at 1/1000 dilution
All lanes: C. elegans embryo lysate at 30 µg
Predicted band size: 15 kDa
Dot Blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)
Dot Blot analysis of Histone H3 (phospho T6, mono methyl K9) using ab272145 at 1/1000 dilution.
Lane 1: Histone H3 1-13.
Lane 2: T6p.
Lane 3: T6pK9Me1.
Lane 4: T6pK9Me2.
Lane 5: T6pK9Me3.
Lane 6: Histone H3 2-17.
Lane 7: K9Me1.
Lane 8: K9Me2.
Lane 9: K9Me3.
Lane 10: K9Ac.
Load: 1, 10, and 100 picomoles of peptide.
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