Anti-Histone H3 (propionyl K23) antibody

• ICC

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Immunocytochemistry - Anti-Histone H3 (propionyl K23) antibody (AB241466)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 10mM sodium propionate for 4 hours) labeling Histone H3 (propionyl K23) with ab241466 at 1/50 dilution in ICC.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (propionyl K23) antibody (AB241466)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 10mM sodium propionate for 4 hours) labeling Histone H3 (propionyl K23) with ab241466 at 1/25 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor^®488-conjugated Goat anti-Rabbit IgG (H+L).

• ChIP

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ChIP - Anti-Histone H3 (propionyl K23) antibody (AB241466)

HeLa ((human epithelial cell line from cervix adenocarcinoma) cells; (4 x 10⁶, treated with 10 mM sodium propionate for 4 hours) were treated with micrococcal nuclease, sonicated, and immunoprecipitated with 5 μg ab241466 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.