AB194684

Anti-Histone H3 (symmetric di methyl R2) antibody

Name: Anti-Histone H3 (symmetric di methyl R2) antibody (ab194684) | Abcam
Brand: Abcam Limited
SKU: AB194684

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(11 Publications)

Rabbit Polyclonal H3 symmetric di methyl R2 antibody. Suitable for Dot, WB, IHC-P, ICC/IF and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human H3-4 symmetric di methyl R2.

View Alternative Names

H3FT, HIST3H3, H3-4, Histone H3.1t, H3/t, H3t, H3/g, Histone H3.4, H3R2me2, H3R2me, H3R2

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Immunofluorescence staining of U-2 OS cells stained for Histone H3 (symmetric di methyl R2) with ab194684 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Paraffin-embedded human stomach tissue stained for Histone H3 (symmetric di methyl R2) using ab194684 at 1/100 dilution in immunohistochemical analysis.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Immunofluorescence staining of C6 cells stained for Histone H3 (symmetric di methyl R2) with ab194684 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Paraffin-embedded mouse brain tissue stained for Histone H3 (symmetric di methyl R2) using ab194684 at 1/100 dilution in immunohistochemical analysis.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Immunofluorescence staining of HIH-3T3 cells stained for Histone H3 (symmetric di methyl R2) with ab194684 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Paraffin-embedded rat liver tissue stained for Histone H3 (symmetric di methyl R2) using ab194684 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Histone H3 (symmetric di methyl R2) antibody (ab194684)

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

Histone H3 protein at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 15 kDa

false

Supplier Data

Dot Blot - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Dot-blot analysis of methylation peptides using ab194684

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Dot, IHC-P

applications

Immunogen

Synthetic Peptide within Human H3-4 symmetric di methyl R2. The exact immunogen used to generate this antibody is proprietary information.

Q16695

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3-4 symmetric di methyl R2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:21 PubMed39849382

2025

AdipoR1 enhances the radiation resistance via ESR1/CCNB1IP1/cyclin B1 pathway in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhan Gan,Linhui Zhu,Yimo Li,Ruoting Ge,Jiahe Tian,Yuxin Chen,Xiang He,Shumei Ma,Xiaodong Liu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2202737 PubMed36941223

2023

The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tianshu Yang,Wei Huang,Tianyu Ma,Xin Yin,Jingyao Zhang,Miaomiao Huo,Ting Hu,Tianyang Gao,Wei Liu,Die Zhang,Hefen Yu,Xu Teng,Min Zhang,Hao Qin,Yunkai Yang,Baowen Yuan,Yan Wang

Nature communications 13:5676 PubMed36167829

2022

TP53 mutations and RNA-binding protein MUSASHI-2 drive resistance to PRMT5-targeted therapy in B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Erazo,Chiara M Evans,Daniel Zakheim,Karen L Chu,Alice Yunsi Refermat,Zahra Asgari,Xuejing Yang,Mariana Da Silva Ferreira,Sanjoy Mehta,Marco Vincenzo Russo,Andrea Knezevic,Xi-Ping Zhang,Zhengming Chen,Myles Fennell,Ralph Garippa,Venkatraman Seshan,Elisa de Stanchina,Olena Barbash,Connie Lee Batlevi,Christina S Leslie,Ari M Melnick,Anas Younes,Michael G Kharas

RSC chemical biology 3:456-467 PubMed35441144

2022

A novel screening strategy to identify histone methyltransferase inhibitors reveals a crosstalk between DOT1L and CARM1.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Si,Corentin Bon,Magdalena Barbachowska,Veronique Cadet-Daniel,Corinne Jallet,Laura Soresinetti,Mikaël Boullé,Magalie Duchateau,Mariette Matondo,Fabrice Agou,Ludovic Halby,Paola B Arimondo

Theranostics 11:9162-9176 PubMed34522232

2021

PRMT5 disruption drives antitumor immunity in cervical cancer by reprogramming T cell-mediated response and regulating PD-L1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yongshuai Jiang,Yuanyang Yuan,Ming Chen,Shengzhe Li,Jun Bai,Yuanteng Zhang,Ying Sun,Guojue Wang,Haiyan Xu,Ziyu Wang,Yingxia Zheng,Hong Nie

Scientific reports 11:10146 PubMed33980975

2021

Symmetrically dimethylated histone H3R2 promotes global transcription during minor zygotic genome activation in mouse pronuclei.

Applications

Unspecified application

Species

Unspecified reactive species

Kohtaro Morita,Yuki Hatanaka,Shunya Ihashi,Masahide Asano,Kei Miyamoto,Kazuya Matsumoto

Life science alliance 4: PubMed33376131

2020

The uncharacterized protein FAM47E interacts with PRMT5 and regulates its functions.

Applications

Unspecified application

Species

Unspecified reactive species

Baskar Chakrapani,Mohd Imran K Khan,Rajashekar Varma Kadumuri,Somlee Gupta,Mamta Verma,Sharad Awasthi,Gayathri Govindaraju,Arun Mahesh,Arumugam Rajavelu,Sreenivas Chavali,Arunkumar Dhayalan

Cell reports 33:108490 PubMed33296647

2020

Nuclear cGAS Functions Non-canonically to Enhance Antiviral Immunity via Recruiting Methyltransferase Prmt5.

Applications

Unspecified application

Species

Unspecified reactive species

Shufang Cui,Qiuya Yu,Lei Chu,Ye Cui,Ming Ding,Quanyi Wang,Hongyun Wang,Yu Chen,Xing Liu,Chen Wang

Immunology 158:304-313 PubMed31509237

2019

Forkhead transcription factor FOXO3a mediates interferon-γ-induced MHC II transcription in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Wu,Zhiwen Fan,Ming Chen,Yi Chen,Danyan Rong,Zhiwei Cui,Yibiao Yuan,Lili Zhuo,Yong Xu

Nature communications 10:3761 PubMed31434880

2019

Genotoxic stress-triggered β-catenin/JDP2/PRMT5 complex facilitates reestablishing glutathione homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Lixue Cao,Geyan Wu,Jinrong Zhu,Zhanyao Tan,Dongni Shi,Xingui Wu,Miaoling Tang,Ziwen Li,Yameng Hu,Shuxia Zhang,Ruyuan Yu,Shuang Mo,Jueheng Wu,Erwei Song,Mengfeng Li,Libing Song,Jun Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (symmetric di methyl R2) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Western blot - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Dot Blot - Anti-Histone H3 (symmetric di methyl R2) antibody (AB194684)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

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