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AB272156

Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody

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(1 Publication)

Rabbit Polyclonal H3 symmetric di methyl R2 + di methyl K4 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Mouse, Caenorhabditis elegans, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3C15.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3C1, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

Immunofluorescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (symmetric di methyl R2, di methyl K4) using ab272156 at 1/100 dilution for 1 hour at room temperature (Green). Dylight 488 secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.

ChIP - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)
  • ChIP

Unknown

ChIP - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

ChIP analysis of chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using 2μg of ab272156 and 20μl of magnetic IgG beads per immunoprecipitation. A "no antibody" (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one

Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)
  • WB

Unknown

Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

Primary incubation overnight at 4°C.

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

All lanes:

Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156) at 1/500 dilution

All lanes:

NIH/3T3 (Mouse embryo fibroblast cell line) histone preparation at 30 µg

Predicted band size: 15 kDa

false

Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)
  • WB

Unknown

Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

Primary incubation overnight at 4°C.

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

All lanes:

Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156) at 1/500 dilution

All lanes:

Caenorhabditis elegans embryo lysate at 30 µg

Predicted band size: 15 kDa

false

Dot Blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (AB272156)

Dot Blot analysis of Histone H3 (symmetric di methyl R2, di methyl K4) using ab272156 at 1/1000 dilution.

Lane 1 : R2Me2.

Lane 2 : R2Me2/K4Me2.

Lane 3 : K4Me2.

Load : 0.1, 1, 0.5, 5, and 10 picomoles of peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Caenorhabditis elegans

Applications

ChIP, Dot, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human H3C15. The exact immunogen used to generate this antibody is proprietary information.

Q71DI3

Specificity

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 symmetric di methyl R2 + di methyl K4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:11059-11070 PubMed33149618

2020

The Pseudogene DUXAP8 Promotes Colorectal Cancer Cell Proliferation, Invasion, and Migration by Inducing Epithelial-Mesenchymal Transition Through Interacting with EZH2 and H3K27me3.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing He,Yi Yu,Wei Huang,Guoliang Feng,Junhe Li
View all publications
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Product promise

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