Rabbit Polyclonal H3 tri methyl K27 antibody. Suitable for Dot, WB, ICC/IF and reacts with Synthetic peptide, Human, Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10. The exact immunogen used to generate this antibody is proprietary information. Database link Q71DI3
Reactivity data
Select an application| Dot | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Synthetic peptide | Tested | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Targets
H31_HUMAN tri methyl K27, H31_HUMAN tri methyl K27
Alternative names
Histone H3.2, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, Histone H3/m, Histone H3/o, H3F2, H3FM, H3C13, HIST2H3C, H3C15, HIST2H3A, HIST2H3D, H3C14, H3K27me3
Recommended products
Rabbit Polyclonal H3 tri methyl K27 antibody. Suitable for Dot, WB, ICC/IF and reacts with Synthetic peptide, Human, Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10.
Alternative names
Histone H3.2, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, Histone H3/m, Histone H3/o, H3F2, H3FM, H3C13, HIST2H3C, H3C15, HIST2H3A, HIST2H3D, H3C14, H3K27me3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10. The exact immunogen used to generate this antibody is proprietary information. Database link Q71DI3
- Purification technique
Affinity purification
- Specificity
A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [Lys27 me3] from other sources has not been determined.
- Concentration
- Purification notes
Anti-Histone H3 [Trimethyl Lys27] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Histone H3 (tri methyl K27) antibody (ab272165)
All lanes: Western blot - Anti-Histone H3 (tri methyl K27) antibody (ab272165) at 1 µg/mL
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3: NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 4: PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 5: A431 (Human epidermoid carcinoma cell line) cell lysate
Predicted band size: 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody (ab272165)
Immunofluorescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (methyl K27) using ab272165 at a 1/50 dilution for 1 hour at room temperature (Green). The secondary antibody FITC secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.
Dot Blot - Anti-Histone H3 (tri methyl K27) antibody (ab272165)
Dot Blot analysis of Histone H3 (methyl K27) using ab272165 at 0.1 μg/ml.
Lane 1: Non Modified Peptide.
Lane 2: Histone H3 K27-Me1 Peptide.
Lane 3: Histone H3 K27-Me2 Peptide.
Lane 4: Histone H3 K27-Me3 Peptide.
Lane 5: Histone H3 K27-Ac Peptide.
Blocking: 5% Blotto at room temperature 30 minutes. Exposure time: 13 seconds.
Downloads
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