AB272165

Anti-Histone H3 (tri methyl K27) antibody

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(3 Publications)

Rabbit Polyclonal H3 tri methyl K27 antibody. Suitable for Dot, WB, ICC/IF and reacts with Synthetic peptide, Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27me3, H3K27me, H3K27

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody (AB272165)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody (AB272165)

Immunofluorescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (methyl K27) using ab272165 at a 1/50 dilution for 1 hour at room temperature (Green). The secondary antibody FITC secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.

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Western blot - Anti-Histone H3 (tri methyl K27) antibody (AB272165)

All lanes:

Western blot - Anti-Histone H3 (tri methyl K27) antibody (ab272165) at 1 µg/mL

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 4:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate

Lane 5:

A431 (Human epidermoid carcinoma cell line) cell lysate

Predicted band size: 15 kDa

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Unknown

Dot Blot - Anti-Histone H3 (tri methyl K27) antibody (AB272165)

Dot Blot analysis of Histone H3 (methyl K27) using ab272165 at 0.1 μg/ml.

Lane 1 : Non Modified Peptide.
Lane 2 : Histone H3 K27-Me1 Peptide.
Lane 3 : Histone H3 K27-Me2 Peptide.
Lane 4 : Histone H3 K27-Me3 Peptide.
Lane 5 : Histone H3 K27-Ac Peptide.

Blocking : 5% Blotto at room temperature 30 minutes. Exposure time : 13 seconds.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Dot, WB

applications

Immunogen

Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10. The exact immunogen used to generate this antibody is proprietary information.

Q71DI3

Specificity

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [Lys27 me3] from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Anti-Histone H3 [Trimethyl Lys27] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 tri methyl K27

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7414 PubMed36460681

2022

Nuclear localization of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Qi Long,Hao Wu,Yanshuang Zhou,Lifan Duan,Hao Yuan,Yingzhe Ding,Yile Huang,Yi Wu,Jinyu Huang,Delong Liu,Baodan Chen,Jian Zhang,Juntao Qi,Shiwei Du,Linpeng Li,Yang Liu,Zifeng Ruan,Zihuang Liu,Zichao Liu,Yifan Zhao,Jianghuan Lu,Junwei Wang,Wai-Yee Chan,Xingguo Liu

Journal of cellular and molecular medicine 26:2529-2542 PubMed35322532

2022

miR-126-5p enhances radiosensitivity of lung adenocarcinoma cells by inhibiting EZH2 via the KLF2/BIRC axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fushi Han,Dongdong Huang,Jinqian Meng,Jiapeng Chu,Meng Wang,Shuzhen Chen

Aging cell 21:e13567 PubMed35146893

2022

Metformin induces S-adenosylmethionine restriction to extend the Caenorhabditis elegans healthspan through H3K4me3 modifiers.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Xiao,Fang Liu,Qinghong Kong,Xinting Zhu,Haijuan Wang,Sanhua Li,Nian Jiang,Changyan Yu,Liu Yun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (tri methyl K27) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody (AB272165)

Western blot - Anti-Histone H3 (tri methyl K27) antibody (AB272165)

Dot Blot - Anti-Histone H3 (tri methyl K27) antibody (AB272165)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Assay Kits

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise