AB195477

Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade

Name: Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477) | Abcam
Brand: Abcam Limited
SKU: AB195477
Price: 515 USD
Availability: InStock

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(19 Publications)

Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, ICC/IF, ChIP, Dot Blot. Suitable for Human, Mouse,.

- Over 10 publications

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27me3, H3K27me, H3K27

5 Images

ChIP - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

ChIP results obtained with ab195477 directed against Histone H3 (tri methyl K27).

ChIP assays were performed using human HeLa cells, ab195477 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes EIF4A2 and GAPDH as negative controls, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3 is preferably present at inactive genes.

ChIP-sequencing - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

ChIP-seq

Supplier Data

ChIP-sequencing - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

ChIPseq results obtained with ab195477 directed against Histone H3 (tri methyl K27).

ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using 1 μg of ab195477. The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure shows the enrichment in genomic regions of chromosome 6, surrounding the TSH2B gene (indicated by an arrow; fig A), of chromosome 20, surrounding the MYT1 gene (fig B), and of chromosome 2 and 3 (figure C and D).

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

Immunofluorescent analysis of NIH 3T3 cells labeling Histone H3 (tri methyl K27) with ab195477 at 1/200 dilution. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with ab195477 in blocking solution followed by an ant-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Supplier Data

Western blot - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

The antibody was diluted in TBS-Tween containing 5% skimmed milk.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477) at 1/500 dilution

Lane 1:

HeLa whole cell extract at 25 µg

Lane 2:

HeLa histone extract at 15 µg

Lane 3:

Recombinant Histone H2A at 1 µg

Lane 4:

Recombinant Histone H2B at 1 µg

Lane 5:

Recombinant Histone H3 at 1 µg

Lane 6:

Recombinant Histone H4 at 1 µg

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Supplier Data

Dot Blot - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

Dot Blot analysis was performed to test the cross reactivity of ab195477 against Histone H3 (tri methyl K27) with peptides containing other modifications of Histone H3 and H4 and the unmodified H3K27 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1/5000. Figure shows a high specificity of the antibody for the modification of interest. Please note that that antibody also recognizes the modification if S28 is phosphorylated.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, ChIP, WB, ChIP-seq, Dot

applications

Immunogen

Synthetic Peptide within Human H3C1 tri methyl K27 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P68431

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Dot Blot in Human, Mouse, samples.

What is the molecular weight of Histone H3?
Anti-Histone H3 (tri methyl K27) - ChIP Grade (ab195477) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.

Trusted by the scientific community
Anti-Histone H3 (tri methyl K27) - ChIP Grade (ab195477) was first used in a scientific publication in 2016 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.05% Sodium azide, 0.05% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 tri methyl K27

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Nature cancer 6:1263-1282 PubMed40588523

2025

Self-amplifying NRF2-EZH2 epigenetic loop converts KRAS-initiated progenitors to invasive pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Antonucci,Na Li,Angeles Duran,Isidoro Cobo,Chiara Nicoletti,Kosuke Watari,Shuvro Prokash Nandi,Feng Zhu,Yongmei Zhao,Irene Riahi,Motoyuki Tsuda,Vidhi M Shah,Terry Morgan,Trent Waugh,Luca Caputo,Yuan Liu,Alexandra Rundberg Nilsson,Hongxu Xian,Jelena Todoric,Li Gu,Elsa Sanchez-Lopez,Guido Eibl,Emily A Vucic,Michal Krawczyk,Qianlan Xu,Andrew M Lowy,Georgia Hatzivassiliou,Merone Roose-Girma,Dorota Skowronska-Krawczyk,David A Scott,Dafna Bar-Sagi,Pablo Tamayo,Ying Wu,Rosalie C Sears,Christopher K Glass,Ludmil B Alexandrov,Pier Lorenzo Puri,David W Dawson,Yinling Hu,Maria T Diaz-Meco,Jorge Moscat,Michael Karin

Cell & bioscience 15:56 PubMed40287726

2025

The chromatin reader Dido3 is a regulator of the gene network that controls B cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Gutiérrez Del Burgo,María Ángeles García-López,Tirso Pons,Enrique Vázquez de Luis,Carlos Martínez-A,Ricardo Villares

Animals : an open access journal from MDPI 14: PubMed39595299

2024

The Germline-Restricted Chromosome of Male Zebra Finches in Meiotic Prophase I: A Proteinaceous Scaffold and Chromatin Modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Sergey Matveevsky

The Plant journal : for cell and molecular biology 120:2437-2455 PubMed39495594

2024

ABI3 regulates ABI1 function to control cell length in primary root elongation zone.

Applications

Unspecified application

Species

Unspecified reactive species

Saptarshi Datta,Drishti Mandal,Sicon Mitra,Swarnavo Chakraborty,Ronita Nag Chaudhuri

Molecular cancer 23:190 PubMed39243015

2024

Unraveling the key role of chromatin structure in cancer development through epigenetic landscape characterization of oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Xue,Lu Liu,Ye Zhang,Yueying He,Jingyao Wang,Zicheng Ma,Tie-Jun Li,Jianyun Zhang,Yanyi Huang,Yi Qin Gao

Nature communications 15:5470 PubMed38937441

2024

Temperature-dependent jumonji demethylase modulates flowering time by targeting H3K36me2/3 in Brassica rapa.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Xin,Peirong Li,Xiuyun Zhao,Yangjun Yu,Weihong Wang,Guihua Jin,Jiao Wang,Liling Sun,Deshuang Zhang,Fenglan Zhang,Shuancang Yu,Tongbing Su

Nucleic acids research 52:4906-4921 PubMed38407438

2024

The jet-like chromatin structure defines active secondary metabolism in fungi.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyong Shao,Jingrui Wang,Yueqi Zhang,Chaofan Zhang,Jie Chen,Yun Chen,Zhangjun Fei,Zhonghua Ma,Xuepeng Sun,Chen Jiao

Antioxidants (Basel, Switzerland) 12: PubMed37627511

2023

Korean Red Ginseng Attenuates Particulate Matter-Induced Senescence of Skin Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoung Ah Kang,Mei Jing Piao,Pincha Devage Sameera Madushan Fernando,Herath Mudiyanselage Udari Lakmini Herath,Joo Mi Yi,Jin Won Hyun

Cell reports 42:112625 PubMed37294634

2023

Co-option of endogenous retroviruses through genetic escape from TRIM28 repression.

Applications

Unspecified application

Species

Unspecified reactive species

Rocio Enriquez-Gasca,Poppy A Gould,Hale Tunbak,Lucia Conde,Javier Herrero,Alexandra Chittka,Christine R Beck,Robert Gifford,Helen M Rowe

Nature genetics 54:684-693 PubMed35551306

2022

Genome-wide analysis of cis-regulatory changes underlying metabolic adaptation of cavefish.

Applications

Unspecified application

Species

Unspecified reactive species

Jaya Krishnan,Chris W Seidel,Ning Zhang,Narendra Pratap Singh,Jake VanCampen,Robert Peuß,Shaolei Xiong,Alexander Kenzior,Hua Li,Joan W Conaway,Nicolas Rohner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade

ChIP - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

ChIP-sequencing - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

Western blot - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

Dot Blot - Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (AB195477)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (19)

Product promise