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AB6002

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade

4

(72 Reviews)

|

(1134 Publications)

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) is a mouse monoclonal antibody detecting Histone H3 in IP, IHC- Wmt, ICC/IF, ELISA, ChIP. Suitable for Cow, Human, Mouse.

- Over 980 publications
- Trusted since 2004

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27me3, H3K27me, H3K27

6 Images
ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
  • ChIP

Unknown

ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 5μg of ab6002 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
  • ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)

ICC/IF image of ab6002 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6002, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5μg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5μg/ml.

ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
  • ChIP

AbReview61399****

ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)

Chromatin was prepared from K562 cells. Cells were fixed with formaldehyde for 10 min. A GATA1 antibody was used as the positive control and a Rabbit IgG was used as the negative control. Incubation with primary antibody was in Immuno Precipitation Dilution buffer for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR.

This image is courtesy of an anonymous abreview.

IHC - Wholemount - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
  • IHC - Wmt

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IHC - Wholemount - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)

In mice, the X-inactivation process is reversed naturally by X-reactivation in blastocysts and germ cells and in culture in pluripotent stem cells. The image shows late blastocyst-stage mouse embryos consisting of three cell types : epiblast (NANOG-positive, cyan), primitive endoderm (GATA4-positive, red) and trophectoderm (CDX2-positive, green). The inactive X-chromosome (H3K27me3-positive, yellow dots) is reactivated only in the epiblast (cells without yellow spots), which will form the embryo. The germ cell factor PRDM14 and the long noncoding RNA Tsix collaborate during the X-reactivation process in blastocysts and pluripotent stem cells and thereby link epigenetic with cellular reprogramming events.

Image is courtesy of Bernhard Payer, runner-up of the immunofluorescence imaging competition 2017.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)

Figure showing the nuclear distribution of H3 (tri-methyl K27) antibody, ab6002 in a) a 46 chromosome, XX cell line, and b) a 49 chromosome, XXXXX cell line.

The location of facultative heterochromatin at the inactive X chromosome is indicated by white arrow heads.

ELISA - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
  • ELISA

Lab

ELISA - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)

All batches of ab6002 are tested in ELISA against peptides to different Histone H3 modifications. Results show strong binding to Histone H3 - tri methyl K27 peptide (ab1782), indicating that this antibody specifically recognizes the Histone H3 tri methyl K27 modification. Weak binding is also detected against the Histone H3 di methyl K27 modification (<12%) (ab1781).

ab17163 - Histone H3 - unmodified
ab1340 - Histone H3 - mono methyl K4
ab7768 - Histone H3 - di methyl K4
ab1342 - Histone H3 - tri methyl K4
ab1771 - Histone H3 - mono methyl K9
ab1772 - Histone H3 - di methyl K9
ab1773 - Histone H3 - tri methyl K9
ab1780 - Histone H3 - mono methyl K27
ab1781 - Histone H3 - di methyl K27
ab1782 - Histone H3 - tri methyl K27

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam 6002

Isotype

IgG3

Light chain type

kappa

Carrier free

No

Reacts with

Human, Mouse, Cow

Applications

ChIP, ELISA, ICC/IF, IHC - Wmt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific for histone H3 tri-methylated at K27. The antibody is blocked in Western blot by tri methyl K27 peptide and slightly by di methyl K27 peptide (there is <12% cross reactivity with di methyl K27 as determined by ELISA). It is not blocked by mono methyl K4, di methyl K4, tri methyl K4, mono methyl K9, di methyl K9, tri methyl K9, mono methyl K27 or unmodified K27 peptides (see the peptide blocking assay blot below).

Reactivity data

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"<p></p>" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Arabidopsis thaliana": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Chicken": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Chinese hamster": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Cow": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "<p></p>" }, "Drosophila melanogaster": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Plants": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Rabbit": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", 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"WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Synthetic peptide - Human": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.025-1 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "notRecommended", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Xenopus laevis": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ELISA-species-checked": "predicted", 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Product details

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) is a mouse monoclonal antibody and is validated for use in ChIP, ELISA, ICC/IF, IHC- Wmt and WB.

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) was first used in a scientific publication in 2002 and has been cited over 982 times in peer reviewed journals. It's performance in ChIP and Western Blot in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has high sensitivity and specificity.

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has 70 independent reviews from customers.

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) specifically detects Histone H3 Tri Methyl-K27 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 100 µg selling sizes.

Top cited antibody for this target with >1000 citations. A highly cited Histone H3 tri methyl K27 antibody, essential for studying epigenetic modifications and gene silencing. This antibody is crucial in chromatin remodeling research, particularly in understanding the role of H3K27me3 in transcription regulation. It is widely used in studies of Polycomb repressive complexes and histone methylation. Histone H3 lysine 27 methylation (H3K27) is a key marker associated with heterochromatin formation and widely used as a marker for transcriptional repression.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 tri methyl K27

Publications (1134)

Recent publications for all applications. Explore the full list and refine your search

Research (Washington, D.C.) 8:0908 PubMed41049615

2025

ARID1A Governs Genomic Stability and Proliferation in SCLC via c-MYC/PARP1 Suppression Driving Vulnerability to BET Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Guozhen Cao,Liying Ma, Xueqin Dai,Peng Hou,Xinhuang Yao,Gongfeng Li,Jiahui Zhang,Ceshi Chen,Wenchu Lin

Journal of orthopaedic surgery and research 20:862 PubMed41024237

2025

KDM6B promotes ferroptosis in rheumatoid arthritis fibroblast-like synoviocytes via the miR-128-3p/SLC7A11 axis through H3K27me3 modification.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wang,Song Gao,Zongwei Liu,Yan Cong,Chuansheng Wu

Genome biology 26:321 PubMed41024142

2025

DeepWheat: predicting the effects of genomic variants on gene expression and regulatory activities across tissues and varieties in wheat using deep learning.

Applications

Unspecified application

Species

Unspecified reactive species

Zhigang Ma,Jiazi Zhang,Hongcui Pei,Yanhong Liu,Hongning Tong,Lei Wang,Zefu Lu

eLife 14: PubMed41026782

2025

Telomeres control human telomerase () expression through non-telomeric TRF2.

Applications

Unspecified application

Species

Unspecified reactive species

Antara Sengupta,Soujanya Vinayagamurthy,Dristhi Soni,Rajlekha Deb,Ananda Kishore Mukherjee,Subhajit Dutta,Jushta Jaiswal,Mukta Yadav,Shalu Sharma,Sulochana Bagri,Shuvra Shekhar Roy,Priya Poonia,Ankita Singh,Divya Khanna,Amit Kumar Kumar Bhatt,Akshay Sharma,Suman Saurav,Rajender K Motiani,Shantanu Chowdhury

Journal of Cancer 16:3629-3642 PubMed40959109

2025

KDM6B enhances anti-PD-L1 immunotherapy efficacy by increasing CD8 T-cell infiltration in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xun,Zehan Liu,Bin Liu,Xiaolin Jiang,Huichao Yang,Jinjin Liu,Botao Wang,Ruifang Gao,Aimin Zhang,Xueliang Wu,Qing Xi,Xiangyang Yu,Qi Zhang

Bio-protocol 15:e5430 PubMed40948898

2025

Simultaneous Capture of Chromatin-Associated RNA and Global RNA-RNA Interactions With Reduced Input Requirements.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Ding,Guoting Chen,Shiping Luan,Yuanyuan Gong,Cuilin Gui,Chen Yang,Zihe Xiang,Junjie Du,Mohamed F Foda,Jiapei Yan,Xingwang Li

Nature communications 16:7545 PubMed40813580

2025

Chromatin retained MUSHER lncRNA integrates ABA and DOG1 signalling pathways to enhance Arabidopsis seeds dormancy.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Przemyslaw Sacharowski,Michal Krzyszton,Lien Brzezniak,Karol Jerzy Rogowski,Miguel Montez,Katarzyna Rosol,Magdalena Wrona,Ruslan Yatusevich,Veena Halale Manjunath,Lukasz Szewc,Artur Jarmolowski,Szymon Swiezewski

Cellular & molecular biology letters 30:95 PubMed40764968

2025

Hypoxia-induced genome-wide DNA demethylation by DNMT3A and EMT of cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Biswanath Chatterjee,Pritha Majumder,Chun-Chang Chen,Jing-Ping Wang,Po-Hsuan Su,Hung-Cheng Lai,Ching-Chen Liu,Hsin-Nan Lin,Chen-Hsin A Yu,Hanna S Yuan,Che-Kun James Shen

Cancer cell international 25:290 PubMed40722171

2025

mA-modified CTC-297N7.9 inhibits hepatocellular carcinoma metastasis via epigenetically downregulating CCL2 and CD47.

Applications

Unspecified application

Species

Unspecified reactive species

Huamei Wei,Changhong Pu,Min Zeng,Rongzhou Lu,Yunyu Wei,Yanyan Huang,Zheng Huang,Lizheng Huang,Zuoming Xu,Jianchu Wang,Rihai Ma,Jian Pu

Bioactive materials 53:58-71 PubMed40688017

2025

Delivering LINE1 antisense oligonucleotides via endothelial targeting extracellular vesicles to ameliorate myocardial infarction-induced cardiac senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Enze Fu,Kai Pan,Benjamin Hinnant,Shang Chen,Zhibo Han,Zhikun Guo,Zhong-Chao Han,Qiong Li,Zongjin Li
View all publications

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