Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade is a mouse monoclonal antibody that is used to detect Histone H3 in ChIP, ELISA, ICC/IF, IHC - Wmt, Western blot. Suitable for Cow, Human, Mouse, Recombinant fragment samples.
- Cited in over 980 publications
- Trusted by researchers since 2004
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
ChIP | ELISA | WB | ICC/IF | IHC - Wmt | |
---|---|---|---|---|---|
Human | Tested | Expected | Tested | Tested | Expected |
Mouse | Expected | Expected | Tested | Expected | Expected |
Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
Arabidopsis thaliana | Predicted | Predicted | Predicted | Predicted | Predicted |
Chicken | Predicted | Predicted | Predicted | Predicted | Predicted |
Chinese hamster | Predicted | Predicted | Predicted | Predicted | Predicted |
Cow | Expected | Expected | Tested | Expected | Expected |
Drosophila melanogaster | Predicted | Predicted | Predicted | Predicted | Predicted |
Plants | Predicted | Predicted | Predicted | Predicted | Predicted |
Rabbit | Predicted | Predicted | Predicted | Predicted | Predicted |
Rhesus monkey | Predicted | Predicted | Predicted | Predicted | Predicted |
Rice | Predicted | Predicted | Predicted | Predicted | Predicted |
Synthetic peptide - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Xenopus laevis | Predicted | Predicted | Predicted | Predicted | Predicted |
Zebrafish | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5.00000-10.00000 µg chromatin for 25 µg chromatin | Notes Use Myo-D ChIP primer pair ab269261 as positive control. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Chinese hamster, Rice, Rat, Rabbit, Chicken | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info 0.02500-1.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Chinese hamster, Rice, Rat, Rabbit, Chicken | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1.00000-5.00000 µg/mL | Notes Blocking: we recommend using 3% milk block for 1 hour at room temperature. This step may need to be optimized for your experiments. |
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes Blocking: we recommend using 3% milk block for 1 hour at room temperature. This step may need to be optimized for your experiments. |
Species Cow | Dilution info 1.00000-5.00000 µg/mL | Notes Blocking: we recommend using 3% milk block for 1 hour at room temperature. This step may need to be optimized for your experiments. |
Species | Dilution info | Notes |
---|---|---|
Species Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Chinese hamster, Rice, Rat, Rabbit, Chicken | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Chinese hamster, Rice, Rat, Rabbit, Chicken | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Chinese hamster, Rice, Rat, Rabbit, Chicken | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
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The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27me3
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade is a mouse monoclonal antibody that is used to detect Histone H3 in ChIP, ELISA, ICC/IF, IHC - Wmt, Western blot. Suitable for Cow, Human, Mouse, Recombinant fragment samples.
- Cited in over 980 publications
- Trusted by researchers since 2004
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
This antibody is specific for histone H3 tri-methylated at K27. The antibody is blocked in Western blot by tri methyl K27 peptide and slightly by di methyl K27 peptide (there is <12% cross reactivity with di methyl K27 as determined by ELISA). It is not blocked by mono methyl K4, di methyl K4, tri methyl K4, mono methyl K9, di methyl K9, tri methyl K9, mono methyl K27 or unmodified K27 peptides (see the peptide blocking assay blot below).
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) is a mouse monoclonal antibody and is validated for use in ChIP, ELISA, ICC/IF, IHC- Wmt and WB.
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) was first used in a scientific publication in 2002 and has been cited over 982 times in peer reviewed journals. It's performance in ChIP and Western Blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has high sensitivity and specificity.
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has 70 independent reviews from customers.
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) specifically detects Histone H3 Tri Methyl-K27 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 100 µg selling sizes.
Top cited antibody for this target with >1000 citations. A highly cited Histone H3 tri methyl K27 antibody, essential for studying epigenetic modifications and gene silencing. This antibody is crucial in chromatin remodeling research, particularly in understanding the role of H3K27me3 in transcription regulation. It is widely used in studies of Polycomb repressive complexes and histone methylation. Histone H3 lysine 27 methylation (H3K27) is a key marker associated with heterochromatin formation and widely used as a marker for transcriptional repression.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 5μg of ab6002 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Chromatin was prepared from K562 cells. Cells were fixed with formaldehyde for 10 min. A GATA1 antibody was used as the positive control and a Rabbit IgG was used as the negative control. Incubation with primary antibody was in Immuno Precipitation Dilution buffer for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR.
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab6002 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) at 1 µg/mL
Lanes 1, 3, 4, 5, 6, 7, 8, 9, 10 and 11: Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µg
Lane 2: Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µg with Human Histone H3 peptide (ab17163)
All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 3min
Histone H3 (tri methyl K27) IHC - Wholemount staining using mouse Anti-Histone H3 (tri methyl K27) antibody
In mice, the X-inactivation process is reversed naturally by X-reactivation in blastocysts and germ cells and in culture in pluripotent stem cells. The image shows late blastocyst-stage mouse embryos consisting of three cell types: epiblast (NANOG-positive, cyan), primitive endoderm (GATA4-positive, red) and trophectoderm (CDX2-positive, green). The inactive X-chromosome (H3K27me3-positive, yellow dots) is reactivated only in the epiblast (cells without yellow spots), which will form the embryo. The germ cell factor PRDM14 and the long noncoding RNA Tsix collaborate during the X-reactivation process in blastocysts and pluripotent stem cells and thereby link epigenetic with cellular reprogramming events.
Image is courtesy of Bernhard Payer, runner-up of the immunofluorescence imaging competition 2017.
All batches of ab6002 are tested in ELISA against peptides to different Histone H3 modifications. Results show strong binding to Histone H3 - tri methyl K27 peptide (Human Histone H3 (tri methyl K27) peptide ab1782), indicating that this antibody specifically recognizes the Histone H3 tri methyl K27 modification. Weak binding is also detected against the Histone H3 di methyl K27 modification (<12%) (Human Histone H3 (di methyl K27) peptide ab1781).
ab17163 - Histone H3 - unmodified
Human Histone H3 (mono methyl K4) peptide ab1340 - Histone H3 - mono methyl K4
Human Histone H3 (di methyl K4) peptide ab7768 - Histone H3 - di methyl K4
Human Histone H3 (tri methyl K4) peptide ab1342 - Histone H3 - tri methyl K4
Human Histone H3 (mono methyl K9) peptide ab1771 - Histone H3 - mono methyl K9
Human Histone H3 (di methyl K9) peptide ab1772 - Histone H3 - di methyl K9
Human Histone H3 (tri methyl K9) peptide ab1773 - Histone H3 - tri methyl K9
Human Histone H3 (mono methyl K27) peptide ab1780 - Histone H3 - mono methyl K27
Human Histone H3 (di methyl K27) peptide ab1781 - Histone H3 - di methyl K27
Human Histone H3 (tri methyl K27) peptide ab1782 - Histone H3 - tri methyl K27
Figure showing the nuclear distribution of H3 (tri-methyl K27) antibody, ab6002 in a) a 46 chromosome, XX cell line, and b) a 49 chromosome, XXXXX cell line.
The location of facultative heterochromatin at the inactive X chromosome is indicated by white arrow heads.
Histone H3 (tri methyl K27) Western blot staining using mouse Anti-Histone H3 (tri methyl K27) antibody
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin (lanes 1-3) and 3% milk (lanes 4-6) before being incubated with ab6002 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) at 1 µg/mL
Lanes 1 and 4: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lanes 2 and 5: EED-/- mouse ES Whole Cell Lysate at 10 µg
Lanes 3 and 6: WT mouse ES Whole Cell Lysate at 10 µg
All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 12min
ICC/IF image of ab6002 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6002, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5μg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5μg/ml.
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