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Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade is a mouse monoclonal antibody that is used to detect Histone H3 in ChIP, ELISA, ICC/IF, IHC - Wmt, Western blot. Suitable for Cow, Human, Mouse, Recombinant fragment samples.

- Cited in over 980 publications
- Trusted by researchers since 2004


Images

ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002), expandable thumbnail
  • ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002), expandable thumbnail
  • Western blot - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002), expandable thumbnail
  • IHC - Wholemount - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002), expandable thumbnail
  • ELISA - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002), expandable thumbnail

Publications

Key facts

Isotype
IgG3
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPELISAWBICC/IFIHC - Wmt
Human
Tested
Expected
Tested
Tested
Expected
Mouse
Expected
Expected
Tested
Expected
Expected
Rat
Predicted
Predicted
Predicted
Predicted
Predicted
Arabidopsis thaliana
Predicted
Predicted
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Predicted
Predicted
Chinese hamster
Predicted
Predicted
Predicted
Predicted
Predicted
Cow
Expected
Expected
Tested
Expected
Expected
Drosophila melanogaster
Predicted
Predicted
Predicted
Predicted
Predicted
Plants
Predicted
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Predicted
Rhesus monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Rice
Predicted
Predicted
Predicted
Predicted
Predicted
Synthetic peptide - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Xenopus laevis
Predicted
Predicted
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
5.00000-10.00000 µg chromatin for 25 µg chromatin
Notes

Use Myo-D ChIP primer pair ab269261 as positive control.

Expected
Expected

Species
Mouse, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Chinese hamster, Rice, Rat, Rabbit, Chicken
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
0.02500-1.00000 µg/mL
Notes

-

Expected
Expected

Species
Human, Mouse, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Chinese hamster, Rice, Rat, Rabbit, Chicken
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1.00000-5.00000 µg/mL
Notes

Blocking: we recommend using 3% milk block for 1 hour at room temperature. This step may need to be optimized for your experiments.

Species
Human
Dilution info
1.00000-5.00000 µg/mL
Notes

Blocking: we recommend using 3% milk block for 1 hour at room temperature. This step may need to be optimized for your experiments.

Species
Cow
Dilution info
1.00000-5.00000 µg/mL
Notes

Blocking: we recommend using 3% milk block for 1 hour at room temperature. This step may need to be optimized for your experiments.

Predicted
Predicted

Species
Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Chinese hamster, Rice, Rat, Rabbit, Chicken
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Chinese hamster, Rice, Rat, Rabbit, Chicken
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Human, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Chinese hamster, Rice, Rat, Rabbit, Chicken
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Recommended products

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade is a mouse monoclonal antibody that is used to detect Histone H3 in ChIP, ELISA, ICC/IF, IHC - Wmt, Western blot. Suitable for Cow, Human, Mouse, Recombinant fragment samples.

- Cited in over 980 publications
- Trusted by researchers since 2004

Key facts

Isotype
IgG3
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
mAbcam 6002
Purification technique
Affinity purification Protein A
Specificity

This antibody is specific for histone H3 tri-methylated at K27. The antibody is blocked in Western blot by tri methyl K27 peptide and slightly by di methyl K27 peptide (there is <12% cross reactivity with di methyl K27 as determined by ELISA). It is not blocked by mono methyl K4, di methyl K4, tri methyl K4, mono methyl K9, di methyl K9, tri methyl K9, mono methyl K27 or unmodified K27 peptides (see the peptide blocking assay blot below).

Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) is a mouse monoclonal antibody and is validated for use in ChIP, ELISA, ICC/IF, IHC- Wmt and WB.

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) was first used in a scientific publication in 2002 and has been cited over 982 times in peer reviewed journals. It's performance in ChIP and Western Blot in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has high sensitivity and specificity.

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has 70 independent reviews from customers.

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) specifically detects Histone H3 Tri Methyl-K27 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 100 µg selling sizes.

Top cited antibody for this target with >1000 citations. A highly cited Histone H3 tri methyl K27 antibody, essential for studying epigenetic modifications and gene silencing. This antibody is crucial in chromatin remodeling research, particularly in understanding the role of H3K27me3 in transcription regulation. It is widely used in studies of Polycomb repressive complexes and histone methylation. Histone H3 lysine 27 methylation (H3K27) is a key marker associated with heterochromatin formation and widely used as a marker for transcriptional repression.

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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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8 product images

  • ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002), expandable thumbnail

    ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)

    Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 5μg of ab6002 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)

    Chromatin was prepared from K562 cells. Cells were fixed with formaldehyde for 10 min. A GATA1 antibody was used as the positive control and a Rabbit IgG was used as the negative control. Incubation with primary antibody was in Immuno Precipitation Dilution buffer for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR.

  • Western blot - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002), expandable thumbnail

    Western blot - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab6002 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) at 1 µg/mL

    Lanes 1, 3, 4, 5, 6, 7, 8, 9, 10 and 11: Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µg

    Lane 2: Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µg with Human Histone H3 peptide (ab17163)

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa

    Observed band size: 17 kDa

    Exposure time: 3min

  • IHC - Wholemount - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002), expandable thumbnail

    IHC - Wholemount - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)

    Histone H3 (tri methyl K27) IHC - Wholemount staining using mouse Anti-Histone H3 (tri methyl K27) antibody

    In mice, the X-inactivation process is reversed naturally by X-reactivation in blastocysts and germ cells and in culture in pluripotent stem cells. The image shows late blastocyst-stage mouse embryos consisting of three cell types: epiblast (NANOG-positive, cyan), primitive endoderm (GATA4-positive, red) and trophectoderm (CDX2-positive, green). The inactive X-chromosome (H3K27me3-positive, yellow dots) is reactivated only in the epiblast (cells without yellow spots), which will form the embryo. The germ cell factor PRDM14 and the long noncoding RNA Tsix collaborate during the X-reactivation process in blastocysts and pluripotent stem cells and thereby link epigenetic with cellular reprogramming events.

    Image is courtesy of Bernhard Payer, runner-up of the immunofluorescence imaging competition 2017.

  • ELISA - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002), expandable thumbnail

    ELISA - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)

    All batches of ab6002 are tested in ELISA against peptides to different Histone H3 modifications. Results show strong binding to Histone H3 - tri methyl K27 peptide (Human Histone H3 (tri methyl K27) peptide ab1782), indicating that this antibody specifically recognizes the Histone H3 tri methyl K27 modification. Weak binding is also detected against the Histone H3 di methyl K27 modification (<12%) (Human Histone H3 (di methyl K27) peptide ab1781).

    ab17163 - Histone H3 - unmodified
    Human Histone H3 (mono methyl K4) peptide ab1340 - Histone H3 - mono methyl K4
    Human Histone H3 (di methyl K4) peptide ab7768 - Histone H3 - di methyl K4
    Human Histone H3 (tri methyl K4) peptide ab1342 - Histone H3 - tri methyl K4
    Human Histone H3 (mono methyl K9) peptide ab1771 - Histone H3 - mono methyl K9
    Human Histone H3 (di methyl K9) peptide ab1772 - Histone H3 - di methyl K9
    Human Histone H3 (tri methyl K9) peptide ab1773 - Histone H3 - tri methyl K9
    Human Histone H3 (mono methyl K27) peptide ab1780 - Histone H3 - mono methyl K27
    Human Histone H3 (di methyl K27) peptide ab1781 - Histone H3 - di methyl K27
    Human Histone H3 (tri methyl K27) peptide ab1782 - Histone H3 - tri methyl K27

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)

    Figure showing the nuclear distribution of H3 (tri-methyl K27) antibody, ab6002 in a) a 46 chromosome, XX cell line, and b) a 49 chromosome, XXXXX cell line.

    The location of facultative heterochromatin at the inactive X chromosome is indicated by white arrow heads.

  • Western blot - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002), expandable thumbnail

    Western blot - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)

    Histone H3 (tri methyl K27) Western blot staining using mouse Anti-Histone H3 (tri methyl K27) antibody

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin (lanes 1-3) and 3% milk (lanes 4-6) before being incubated with ab6002 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) at 1 µg/mL

    Lanes 1 and 4: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

    Lanes 2 and 5: EED-/- mouse ES Whole Cell Lysate at 10 µg

    Lanes 3 and 6: WT mouse ES Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa

    Observed band size: 17 kDa

    Exposure time: 12min

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)

    ICC/IF image of ab6002 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6002, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5μg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5μg/ml.

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Product protocols

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