Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade
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(72 Reviews)
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(1134 Publications)
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) is a mouse monoclonal antibody detecting Histone H3 in IP, IHC- Wmt, ICC/IF, ELISA, ChIP. Suitable for Cow, Human, Mouse.
- Over 980 publications
- Trusted since 2004
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H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27me3, H3K27me, H3K27
- ChIP
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ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 5μg of ab6002 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
- ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
ICC/IF image of ab6002 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6002, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5μg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5μg/ml.
- ChIP
AbReview61399****
ChIP - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
Chromatin was prepared from K562 cells. Cells were fixed with formaldehyde for 10 min. A GATA1 antibody was used as the positive control and a Rabbit IgG was used as the negative control. Incubation with primary antibody was in Immuno Precipitation Dilution buffer for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR.
This image is courtesy of an anonymous abreview.
- IHC - Wmt
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IHC - Wholemount - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
In mice, the X-inactivation process is reversed naturally by X-reactivation in blastocysts and germ cells and in culture in pluripotent stem cells. The image shows late blastocyst-stage mouse embryos consisting of three cell types : epiblast (NANOG-positive, cyan), primitive endoderm (GATA4-positive, red) and trophectoderm (CDX2-positive, green). The inactive X-chromosome (H3K27me3-positive, yellow dots) is reactivated only in the epiblast (cells without yellow spots), which will form the embryo. The germ cell factor PRDM14 and the long noncoding RNA Tsix collaborate during the X-reactivation process in blastocysts and pluripotent stem cells and thereby link epigenetic with cellular reprogramming events.
Image is courtesy of Bernhard Payer, runner-up of the immunofluorescence imaging competition 2017.
- ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
Figure showing the nuclear distribution of H3 (tri-methyl K27) antibody, ab6002 in a) a 46 chromosome, XX cell line, and b) a 49 chromosome, XXXXX cell line.
The location of facultative heterochromatin at the inactive X chromosome is indicated by white arrow heads.
- ELISA
Lab
ELISA - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)
All batches of ab6002 are tested in ELISA against peptides to different Histone H3 modifications. Results show strong binding to Histone H3 - tri methyl K27 peptide (ab1782), indicating that this antibody specifically recognizes the Histone H3 tri methyl K27 modification. Weak binding is also detected against the Histone H3 di methyl K27 modification (<12%) (ab1781).
ab17163 - Histone H3 - unmodified
ab1340 - Histone H3 - mono methyl K4
ab7768 - Histone H3 - di methyl K4
ab1342 - Histone H3 - tri methyl K4
ab1771 - Histone H3 - mono methyl K9
ab1772 - Histone H3 - di methyl K9
ab1773 - Histone H3 - tri methyl K9
ab1780 - Histone H3 - mono methyl K27
ab1781 - Histone H3 - di methyl K27
ab1782 - Histone H3 - tri methyl K27
Reactivity data
Product details
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) is a mouse monoclonal antibody and is validated for use in ChIP, ELISA, ICC/IF, IHC- Wmt and WB.
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) was first used in a scientific publication in 2002 and has been cited over 982 times in peer reviewed journals. It's performance in ChIP and Western Blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has high sensitivity and specificity.
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has 70 independent reviews from customers.
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) specifically detects Histone H3 Tri Methyl-K27 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 100 µg selling sizes.
Top cited antibody for this target with >1000 citations. A highly cited Histone H3 tri methyl K27 antibody, essential for studying epigenetic modifications and gene silencing. This antibody is crucial in chromatin remodeling research, particularly in understanding the role of H3K27me3 in transcription regulation. It is widely used in studies of Polycomb repressive complexes and histone methylation. Histone H3 lysine 27 methylation (H3K27) is a key marker associated with heterochromatin formation and widely used as a marker for transcriptional repression.
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Publications (1134)
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Research (Washington, D.C.) 8:0908 PubMed41049615
2025
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Journal of orthopaedic surgery and research 20:862 PubMed41024237
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Genome biology 26:321 PubMed41024142
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eLife 14: PubMed41026782
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Journal of Cancer 16:3629-3642 PubMed40959109
2025
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Bio-protocol 15:e5430 PubMed40948898
2025
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Nature communications 16:7545 PubMed40813580
2025
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Cellular & molecular biology letters 30:95 PubMed40764968
2025
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Cancer cell international 25:290 PubMed40722171
2025
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Bioactive materials 53:58-71 PubMed40688017
2025
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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