AB9050

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade

Name: Histone H3 (tri methyl K36) antibody, ChIP grade (ab9050) | Abcam
Brand: Abcam Limited
SKU: AB9050
Price: 125 USD
Availability: InStock
Rating: 5 (64 reviews)

(64 Reviews)

(975 Publications)

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, ICC/IF, ChIP. Suitable for Cow, Human.

- Over 970 publications
- Trusted since 2004

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K36me3, H3K36me, H3K36

5 Images

ChIP - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ChIP

Lab

ChIP - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Chromatin was prepared from U-2 OS (Human bone osteosarcoma epithelial cell line) cells according to the Abcam X-ChIP protocol.

Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 2 μg of ab9050 (blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH (active) and MYO-D (inactive) promoters and over the γ-Actin gene (active).

Schematic diagram of the γ-Actin gene is shown on the top of the figure.

Black boxes represent exons and thin lines represent introns.

PCR products are depicted as bars under the gene.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ICC/IF

AbReview29364****

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ab9050 staining Histone H3 (tri methyl K36) in Saos-2 (Human osteosarcoma cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/1000) for 1 hour. An Alexa Fluor^®488-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ab9050 staining Histone H3 (tri-methyl K36) in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

The cells were fixed with 100% methanol for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab9050 at 0.1 μg/ml and ab7291 (anti beta-Tubulin) at 1 μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1 hour with a goat anti-rabbit AlexaFluor^®488 secondary (ab150081) at 2 μg/ml (shown in green) and a goat anti-mouse AlexaFluor^®594 (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labeled in blue with DAPI.

Negative controls : 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ab9050 stained in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

Cells were fixed with 100% methanol for 5 minutes at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9050 at 0.1 μg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150120) (pseudo-colored red) and Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150081) secondary antibody (colored green) used at 1 μg/ml for 1 hour at room temperature.

DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 μM for 1 hour at room temperature.

Project

Western blot - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

All lanes:

Western blot - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) at 1 µg/mL

Lanes 1, 4 and 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide - unmodified K36

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K36) peptide (<a href='/en-us/products/unavailable/human-histone-h3-mono-methyl-k36-peptide-ab1783'>ab1783</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K37) peptide (<a href='/en-us/products/unavailable/human-histone-h3-tri-methyl-k37-peptide-ab24417'>ab24417</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Cow, Human

Applications

WB, ICC/IF, ChIP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.1-1 µg/mL", "ICCIF-species-notes": "We recommend using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-alexa-fluor-488-preadsorbed-ab150081'>ab150081</a>) secondary antibody." }, "Mouse": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Arabidopsis thaliana": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Plants": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rice": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Saccharomyces cerevisiae": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Schizosaccharomyces pombe": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Silk worm": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Trypanosoma brucei": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Have you thought about this alternative?

AB282572

Anti-Histone H3 (tri methyl K36) antibody [EPR23525-232] - ChIP Grade

1 Reviews

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Why is this recommended?

We’re constantly upgrading our range to offer the highest quality products. We have selected this alternative product for your review because it gives improved performance, is easier and more convenient to use, or is available faster.

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Product details

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 tri methyl K36

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Publications (975)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:8238 PubMed38086830

2023

Epigenetic modifications regulate cultivar-specific root development and metabolic adaptation to nitrogen availability in wheat.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Zhiyuan Jin,Fa Cui,Long Zhao,Xiaoyu Zhang,Jinchao Chen,Jing Zhang,Yanyan Li,Yongpeng Li,Yanxiao Niu,Wenli Zhang,Caixia Gao,Xiangdong Fu,Yiping Tong,Lei Wang,Hong-Qing Ling,Junming Li,Jun Xiao

PLoS biology 21:e3002417 PubMed38048343

2023

FMRP deficiency leads to multifactorial dysregulation of splicing and mislocalization of MBNL1 to the cytoplasm.

Applications

Unspecified application

Species

Unspecified reactive species

Suna Jung,Sneha Shah,Geongoo Han,Joel D Richter

Communications biology 6:1225 PubMed38044380

2023

Organ-specific characteristics govern the relationship between histone code dynamics and transcriptional reprogramming during nitrogen response in tomato.

Applications

Unspecified application

Species

Unspecified reactive species

Russell Julian,Ryan M Patrick,Ying Li

Life science alliance 7: PubMed38011998

2023

2-Hydroxyglutarate modulates histone methylation at specific loci and alters gene expression via Rph1 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Marios Gavriil,Marco Proietto,Nicole Paczia,Aurelien Ginolhac,Rashi Halder,Elena Valceschini,Thomas Sauter,Carole L Linster,Lasse Sinkkonen

Scientific reports 13:19885 PubMed37963940

2023

SETD5 regulates the OGT-catalyzed O-GlcNAcylation of RNA polymerase II, which is involved in the stemness of colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hye In Cho,Sora Jo,Min Seong Kim,Han Byeol Kim,Xingzhe Liu,Yanhua Xuan,Jin Won Cho,Yeun Kyu Jang

Clinical and translational medicine 13:e1475 PubMed37962020

2023

Loss of SETD2 aggravates colorectal cancer progression caused by SMAD4 deletion through the RAS/ERK signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxiao Ma,Min Liu,Wenxin Feng,Hanyu Rao,Wei Zhang,Changwei Liu,Yue Xu,Ziyi Wang,Yan Teng,Xiao Yang,Li Ni,Jin Xu,Wei-Qiang Gao,Bing Lu,Li Li

Life science alliance 7: PubMed37940188

2023

DCAF14 regulates CDT2 to promote SET8-dependent replication fork protection.

Applications

Unspecified application

Species

Unspecified reactive species

Neysha Tirado-Class,Caitlin Hathaway,Anthony Nelligan,Thuan Nguyen,Huzefa Dungrawala

The New phytologist 241:267-282 PubMed37849024

2023

R-loops act as regulatory switches modulating transcription of COLD-responsive genes in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Zexue He,Mengqi Li,Xiucai Pan,Yulian Peng,Yining Shi,Qi Han,Manli Shi,Linwei She,Gennadii Borovskii,Xiaojun Chen,Xiaofeng Gu,Xuejiao Cheng,Wenli Zhang

Science advances 9:eadg5109 PubMed37831776

2023

Coinhibition of topoisomerase 1 and BRD4-mediated pause release selectively kills pancreatic cancer via readthrough transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Donald P Cameron,Jan Grosser,Swetlana Ladigan,Vladislav Kuzin,Evanthia Iliopoulou,Anika Wiegard,Hajar Benredjem,Kathryn Jackson,Sven T Liffers,Smiths Lueong,Phyllis F Cheung,Deepak Vangala,Michael Pohl,Richard Viebahn,Christian Teschendorf,Heiner Wolters,Selami Usta,Keyi Geng,Claudia Kutter,Marie Arsenian-Henriksson,Jens T Siveke,Andrea Tannapfel,Wolff Schmiegel,Stephan A Hahn,Laura Baranello

Nature 622:619-626 PubMed37758950

2023

Inhibition of fatty acid oxidation enables heart regeneration in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Li,Fan Wu,Stefan Günther,Mario Looso,Carsten Kuenne,Ting Zhang,Marion Wiesnet,Stephan Klatt,Sven Zukunft,Ingrid Fleming,Gernot Poschet,Astrid Wietelmann,Ann Atzberger,Michael Potente,Xuejun Yuan,Thomas Braun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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primary-antibodies

histone-h3-tri-methyl-k27-antibody-epr18607-chip-grade-ab192985

(14 reviews)

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