AB9050

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade

Name: Histone H3 (tri methyl K36) antibody, ChIP grade (ab9050) | Abcam
Brand: Abcam Limited
SKU: AB9050
Price: 180 USD
Availability: InStock
Rating: 5 (64 reviews)

(64 Reviews)

(1093 Publications)

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, ICC/IF, ChIP. Suitable for Cow, Human.

- Over 970 publications
- Trusted since 2004

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K36me3, H3K36me, H3K36

5 Images

ChIP - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ChIP

Lab

ChIP - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Chromatin was prepared from U-2 OS (Human bone osteosarcoma epithelial cell line) cells according to the Abcam X-ChIP protocol.

Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 2 μg of ab9050 (blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH (active) and MYO-D (inactive) promoters and over the γ-Actin gene (active).

Schematic diagram of the γ-Actin gene is shown on the top of the figure.

Black boxes represent exons and thin lines represent introns.

PCR products are depicted as bars under the gene.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ICC/IF

AbReview29364****

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ab9050 staining Histone H3 (tri methyl K36) in Saos-2 (Human osteosarcoma cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/1000) for 1 hour. An Alexa Fluor^®488-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ab9050 staining Histone H3 (tri-methyl K36) in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

The cells were fixed with 100% methanol for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab9050 at 0.1 μg/ml and ab7291 (anti beta-Tubulin) at 1 μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1 hour with a goat anti-rabbit AlexaFluor^®488 secondary (ab150081) at 2 μg/ml (shown in green) and a goat anti-mouse AlexaFluor^®594 (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labeled in blue with DAPI.

Negative controls : 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

ab9050 stained in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

Cells were fixed with 100% methanol for 5 minutes at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9050 at 0.1 μg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150120) (pseudo-colored red) and Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150081) secondary antibody (colored green) used at 1 μg/ml for 1 hour at room temperature.

DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 μM for 1 hour at room temperature.

Project

Western blot - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

All lanes:

Western blot - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide - unmodified K36

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K36) peptide (<a href='/en-us/products/unavailable/human-histone-h3-mono-methyl-k36-peptide-ab1783'>ab1783</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K36) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k36-peptide-ab1784'>ab1784</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K36) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k36-peptide-ab1785'>ab1785</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K37) peptide (<a href='/en-us/products/unavailable/human-histone-h3-tri-methyl-k37-peptide-ab24417'>ab24417</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Cow, Human

Applications

ICC/IF, WB, ChIP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.1-1 µg/mL", "ICCIF-species-notes": "We recommend using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-alexa-fluor-488-preadsorbed-ab150081'>ab150081</a>) secondary antibody." }, "Mouse": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Arabidopsis thaliana": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Plants": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rice": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Saccharomyces cerevisiae": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Schizosaccharomyces pombe": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Silk worm": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Trypanosoma brucei": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) is a rabbit polyclonal antibody and is validated for use in ChIP, ICC/IF, WB in cow, human, saccharomyces cerevisiae samples.

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) has been cited over 975 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) has high sensitivity and specificity.

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) has 64 independent reviews from customers.

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) specifically detects Histone H3 Tri Methyl-K36 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 µg selling sizes.

H3K36me3 is a crucial epigenetic modification that involves the tri-methylation of lysine 36 on Histone H3, playing a significant role in gene regulation and DNA repair. Utilizing our tri methyl K36 antibody can significantly enhance the detection and study of this modification. Research indicates that H3K36me3 is associated with various cancers, making it a vital target for understanding disease mechanisms and developing new therapeutic strategies. Abnormal H3K36me3 levels are linked to cancer progression and poor prognosis. Studying H3K36me3 modifications can also provide insights into potential therapeutic targets for conditions like congenital heart defects and neurological disorders.

For detection of methylated histone H3.

Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 tri methyl K36

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Publications (1093)

Recent publications for all applications. Explore the full list and refine your search

BMC microbiology 25:644 PubMed41068631

2025

β-caryophyllene regulates H3K36me3 to inhibit conidial germination and mycelial growth of Fusarium proliferatum.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Zhu,Tian-Tian Li,Shi-Wei Zhou,Xue-Jie Qin,You Li,Sulukkana Noiprasert,Kanyaphat Apiwongsrichai,Fu-Rong Xu,Xiao-Yun Liu,Xian Dong

Nature cell biology 27:1938-1951 PubMed41028835

2025

Phase-separated NDF-FACT condensates facilitate transcription elongation on chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwei Li,Francesca Burgos-Bravo,Kevin Xu,Chen Li,Kelvin Y Kwan,Alexander B Tong,Zelin Shan,Huan Wang,Motoki Takaku,Joey Li,Zheng Shi,Dmitry Lyumkis,Carlos Bustamante,Jia Fei

Genome biology 26:321 PubMed41024142

2025

DeepWheat: predicting the effects of genomic variants on gene expression and regulatory activities across tissues and varieties in wheat using deep learning.

Applications

Unspecified application

Species

Unspecified reactive species

Zhigang Ma,Jiazi Zhang,Hongcui Pei,Yanhong Liu,Hongning Tong,Lei Wang,Zefu Lu

Genome biology 26:303 PubMed40999518

2025

Land plant-specific H3K27 methyltransferases ATXR5 and ATXR6 control plant development and stress responses.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyi Li,Jie Pan,Qian Liu,Huairen Zhang,Hui Li,Danhua Jiang

Nucleic acids research 53: PubMed40867051

2025

A direct interaction between the Chd1 CHCT domain and Rtf1 controls Chd1 distribution and nucleosome positioning on active genes.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah A Tripplehorn,Santana M Lardo,Margaret K Shirra,Hannah G Marvil,Sarah J Hainer,Karen M Arndt

Nature communications 16:8018 PubMed40866413

2025

Hypoxic stress incites HIF1α-driven ribosome biogenesis that can be exploited by targeting RNA Polymerase I.

Applications

Unspecified application

Species

Unspecified reactive species

Amr Elhamamsy,Brandon J Metge,Courtney A Swain,Mohamed H Elbahoty,Dominique C Hinshaw,Sarah C Kammerud,Dongquan Chen,Rajeev S Samant,Lalita A Shevde

Cellular & molecular biology letters 30:95 PubMed40764968

2025

Hypoxia-induced genome-wide DNA demethylation by DNMT3A and EMT of cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Biswanath Chatterjee,Pritha Majumder,Chun-Chang Chen,Jing-Ping Wang,Po-Hsuan Su,Hung-Cheng Lai,Ching-Chen Liu,Hsin-Nan Lin,Chen-Hsin A Yu,Hanna S Yuan,Che-Kun James Shen

Viruses 17: PubMed40733511

2025

Expression and Localization of a New Parvovirus-Derived Protein in the Guinea Pig.

Applications

Unspecified application

Species

Unspecified reactive species

Camila E Osega,Fernando J Bustos,Francisca C Bronfman,Robert J Gifford,Gloria Arriagada

Nature communications 16:6900 PubMed40715155

2025

SMCHD1 maintains heterochromatin, genome compartments and epigenome landscape in human myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Huang,Wei Cui,Ishara Ratnayake,Kristin L Gallik,Lorna Cohen,Rabi Tawil,Gerd P Pfeifer

JCI insight 10: PubMed40663488

2025

NSD1-916aa encoded by CircNSD1 contributes to AKI-to-CKD transition through inducing ferroptosis in tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Gao,Junsheng Zhang,Chaoyi Chen,Sai Zhu,Xianglong Wei,Guiqin Tang,Sheng Wang,Yukai Wang,Xinran Liu,Ling Jiang,Yonggui Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade

ChIP - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Western blot - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (AB9050)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1093)

Product promise