AB272164

Anti-Histone H3 (tri methyl K4, acetyl K9) antibody

Name: Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (ab272164) | Abcam
Brand: Abcam Limited
SKU: AB272164

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Rabbit Polyclonal H3 tri methyl K4 + acetyl K9 antibody. Suitable for Dot, WB, ICC/IF and reacts with Synthetic peptide, Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10.

View Alternative Names

HIST2H3A, H3C14, H3F2, H3FM, HIST2H3C, H3C13, HIST2H3D, H3C15, Histone H3.2, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, Histone H3/m, Histone H3/o

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (AB272164)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (AB272164)

Immunofluorescent analysis of HeLa cells labeling Histone H3 (tri methyl K4, acetyl K9) using using ab272164 at 1/10 dilution (Green). The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.

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Western blot - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (AB272164)

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : 15 kDa

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Western blot - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (ab272164)

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Western blot - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (ab272164) at 1 µg/mL

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Histone H3 nuclear marker

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NIH/3T3 cell lysate at 35 µg

Predicted band size: 15 kDa

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Dot Blot - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (AB272164)

Dot Blot analysis of H3 (tri methyl K4, acetyl K9) using ab272164 at 1/1000 dilution.

Lane 1 : K4/K9 unmodified.

Lane 2 : K4-Me3/K9-Ac.

Lane 3 : K4-Me1.

Lane 4 : K4-Me2.

Lane 5 : K4-Me3.

Lane 6 : K9-Ac.

Load : 1, 10, and 100 picomoles of peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Dot, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human H3C15 di methyl K36 + K36 + tri methyl K27 + K27 + K79 + K79 + K4 + acetyl K9 + K9 + phospho S10. The exact immunogen used to generate this antibody is proprietary information.

Q71DI3

Specificity

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 K4-Me3/K9-Ac from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10", "ICCIF-species-notes": "" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Anti-Histone H3 [Trimethyl Lys4/ac Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C15 tri methyl K4 + acetyl K9

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (tri methyl K4, acetyl K9) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (AB272164)

Western blot - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (AB272164)

Dot Blot - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (AB272164)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise