AB8580

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

Name: Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) | Abcam
Brand: Abcam Limited
SKU: AB8580
Price: 450 USD
Availability: InStock
Rating: 5 (90 reviews)

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(90 Reviews)

(2434 Publications)

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ChIP, PepArr. Suitable for Cow, Human.

- Over 2130 publications
- Trusted since 2002

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me3, H3K4me, H3K4

11 Images

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

ChIP

Unknown

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Chromatin was prepared from U-2 OS (Human bone osteosarcoma epithelial cell line) cells according to the Abcam X-ChIP protocol.

Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 2 μg of ab8580 (blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

IHC image of ab8580 staining Histone H3 (tri methyl K4) in human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab8580, 1/500 dilution, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

No primary antibody was used in the negative control (inset).

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

ab8580 staining Histone H3 (tri methyl K4) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab8580 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

ab8580 staining Histone H3 (tri methyl K4) in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

All cells were fixed with 100% methanol (5 minutes) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab4729 at 1/1000 and ab7291 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1 hour with goat anti-rabbit Alexa-Fluor^®488 secondary (ab150077) at 2 μg/ml (shown in green) and goat anti-mouse Alexa-Fluor^®594 secondary (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labeled in blue with DAPI.

Negative controls : 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no non-specific reaction between primary and secondary antibodies used.

ICC/IF

AbReview9180****

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

ab8580 staining cultured human primary fibroblasts by ICC.

Cells were fixed in PFA and permeabilized in Triton X-100 and saponin prior to blocking with 1% BSA for 1 hour at RT. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. An FITC-conjugated rabbit anti-rabbit IgG antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Eva Bartova

ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Human female lymphoblast immunostained with ab8580 (1 : 100) (yellowish green) specific for histone H3 lysine 4 (H3-K4) trimethylation; the DNA is stained red with propidium iodide (PI).

Note the inactive X chromosome (arrow) and pericentromeric heterochromatin are largely devoid of this modification.

This image is courtesy of Ahmad Khalil and Daniel Driscoll, University of Florida College of Medicine.

ChIP

AbReview57110****

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Chromatin was prepared from human cell lysate - nuclear B cells according to the Abcam X-ChIP protocol.

Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 0.5 μg of ab8580 per μg chromatin in ChIP Buffer fot 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region. Negative control : IgG and Gene Desert. Positive control : GAPDH Promoter.

This image is courtesy of an anonymous Abreview

ChIP

AbReview75502****

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

ChIP was performed with human thyroid cancer cell lysate and 1 µg/µg of ab8580. Lysates were incubated with the primary antibody for 16 hours at 4°C. positive control promoter : GAPDH promoter; positive control enhancer : enhancer region of a published target (10.1093/nar/gkx802); negative control : published negative region (10.1093/nar/gkx802).

This image is courtesy of an anonymous Abreview

ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Staining (green) with the anti-trimethyl Lysine K4 of Histone H3 antibody (ab8580) shows ringing of regions that appear as nucleoplasmic holes. These represent the positions of splicing factor compartments, which are preferentially associated with active genes and highly acetylated histone H3.

The antibody, as expected, fails to stain heterochromatin (red).

This image is courtesy of Kirk McManus in the lab of Michael Hendzel, University of Alberta

Project8180****

Western blot - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

All lanes:

Western blot - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) at 1 µg/mL

All lanes:

Calf thymus histone preparation (nuclear lysate) at 0.5 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L) HRP- conjugated antibody at 1/50000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 8min

Peptide Array - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

PepArr

Unknown

Peptide Array - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

All batches of ab8580 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - tri methyl K4 peptide (ab1342), indicating that this antibody specifically recognises the Histone H3 - tri methyl K4 modification. Slight cross reactivity is observed with the Histone H3 - di methyl K4 modification. Optimization is recommended to avoid array signal saturation.

ab1340 - Histone H3 - mono methyl K4
ab1342 - Histone H3 - tri methyl K4
ab1771 - Histone H3 - mono methyl K9
ab1772 - Histone H3 - di methyl K9
ab1773 - Histone H3 - tri methyl K9
ab1780 - Histone H3 - mono methyl K27
ab1781 - Histone H3 - di methyl K27
ab1782 - Histone H3 - tri methyl K27
ab7228 - Histone H3 - unmodified
ab7768 - Histone H3 - di methyl K4

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Cow, Human

Applications

IHC-P, ICC/IF, ChIP, WB, PepArr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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href='/en-us/products/reagents/gapdh-positive-control-chip-primer-pair-ab267832'>ab267832</a> as positive control.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "1/100 - 1/5000" }, "Mouse": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Arabidopsis thaliana": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Common marmoset": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Indian muntjac": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mammals": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Oikopleura": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Plants": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rice": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Saccharomyces cerevisiae": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide - Human": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "2 µg/mL", "PepArr-species-notes": "Slight cross reactivity is observed with the Histone H3 - di methyl K4 modification. Optimisation is recommended to avoid array signal saturation.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Tetrahymena": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Trypanosoma cruzi": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) is a rabbit polyclonal antibody and is validated for use in ChIP, ICC/IF, IHC-P, PepArr and WB.

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) was first used in a scientific publication in 2002 and has been cited over 2135 times in peer reviewed journals. It's performance in ChIP in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) has high sensitivity and specificity.

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) has 92 independent reviews from customers.

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) specifically detects Histone H3 Tri Methyl-K4 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 50 µg selling sizes.

Top cited antibody for this target with >2000 citations and >60 five star abreviews. Trimethylation of histone H3 (H3K4me3) is a key epigenetic mark that consistently occurs at transcription start sites, promoting higher transcription activity. H3K4me3 is considered a marker and used as a control for gene activation. It plays a crucial role in cell identity during pre-implantation development and in deriving embryonic stem cells from the inner cell mass and trophoblast stem cells from the trophectoderm. Histone H3 lysine 4 trimethylation (H3K4me3) plays a significant role in cancer by regulating gene expression and chromatin structure.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 tri methyl K4

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Publications (2434)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8955 PubMed41062456

2025

Fosl2 facilitates chromatin accessibility to determine developmental events during follicular maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyong Zhang,Zechen Li,Yanmei Zhu,Wencong Lyu,Wenlu Wei,Haochen Wang,Shuangjie Tian,Wei Yue,Jiajing Zhong,Qing-Yuan Sun,Yiting Guan

Genome biology 26:337 PubMed41053909

2025

A nucleoporin-associated signaling cascade controls plant immunity via histone modification.

Applications

Unspecified application

Species

Unspecified reactive species

Leiwen Pan,Shun Peng,Yuehui Zhang,Huan Chang,Yi Yang,Dongbei Guo,Yuan Guo,Yakun Han,Ting Mao,Yuchen Huang,Shui Wang

Cell communication and signaling : CCS 23:397 PubMed40993686

2025

Parental S-adenosylmethionine diet defines offspring immune response via histone H3K4me3 complex and Endoplasmic Reticulum UPR.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Liu,Qingyao Wang,Jun Xiong,Mengqi Wang,Hanlin Zhou,Yi Xiao

PLoS genetics 21:e1011608 PubMed40971994

2025

Epigenome and transcriptome changes in KMT2D-related Kabuki syndrome Type 1 iPSCs, neuronal progenitors and cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Cuvertino,Evgenii Martirosian,Kedar Bhosale,Peiwen Cheng,Terence Garner,Ian J Donaldson,Adam Jackson,Adam Stevens,Andrew D Sharrocks,Susan J Kimber,Siddharth Banka

Bio-protocol 15:e5430 PubMed40948898

2025

Simultaneous Capture of Chromatin-Associated RNA and Global RNA-RNA Interactions With Reduced Input Requirements.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Ding,Guoting Chen,Shiping Luan,Yuanyuan Gong,Cuilin Gui,Chen Yang,Zihe Xiang,Junjie Du,Mohamed F Foda,Jiapei Yan,Xingwang Li

Cancer research : PubMed40939188

2025

Hypoxia Induces Immunosuppression by Silencing STING in Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhong Lu,Annali M Yurkevicz,Yanfeng Liu,Peter M Glazer

PLoS biology 23:e3003362 PubMed40934285

2025

The mTORC2 subunit RICTOR drives breast cancer progression by promoting ganglioside biosynthesis through transcriptional and epigenetic mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Nafees Ansari,Somesh K Jha,Ali Khan,Kajal Rajput,Nishant Pandey,Dolly Jain,Rajeshwari Tripathi,Nihal Medatwal,Pankaj Sharma,Sudeshna Datta,Animesh Kar,Trishna Pani,Sk Asif Ali,Kaushavi Cholke,Kajal Rana,Valiya P Snijesh,Geetashree Mukherjee,Suryanarayana V S Deo,Soumen Basak,Ashutosh Mishra,Jyothi S Prabhu,Arnab Mukhopadhyay,Avinash Bajaj,Ujjaini Dasgupta

Nature cell biology 27:1411-1421 PubMed40897805

2025

Genome-wide CRISPR screen identifies Menin and SUZ12 as regulators of human developmental timing.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Xu,Hyein S Cho,James O S Hackland,Silvia Benito-Kwiecinski,Nathalie Saurat,Oliver Harschnitz,Marco Vincenzo Russo,Ralph Garippa,Gabriele Ciceri,Lorenz Studer

Nature genetics 57:2264-2275 PubMed40890361

2025

Chromatin dynamics of a large-sized genome provides insights into polyphenism and X0 dosage compensation of locusts.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Liu,Feng Jiang,Ran Li,Shanlin Liu,Wenjing Fang,Xiao Li,Ting Hu,Lianyun Feng,Jie Zhang,Zhikang Liu,Jing He,Wei Guo,Xianhui Wang,Zongyi Sun,Jingjing Li,Yunan Gao,Jiacheng Yi,Qiye Li,Xiaoxiao Wang,Liya Wei,Le Kang

Communications biology 8:1311 PubMed40883562

2025

Deficiency of KMT2D causes autistic-like behavior in mice and zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Huakun Shangguan,Jingguo Huang,Xia Wei,Jie Huang,Zaicheng Cao,Xingjingyan Chen,Jiahui Yuan,Jingrun Zhang,Binbin Ye,Ruofei Yan,Ruimin Chen,Wucheng Tao

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Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Western blot - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

Peptide Array - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

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