AB313500

Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade - BSA and Azide free

RabMAb
Advanced Validation
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal H3 tri methyl K4 antibody. Carrier free. Suitable for CUT&Tag, ChIC/CUT&RUN-seq, WB, ICC/IF, Dot, PepArr, IP, ChIP, ChIP-seq, Flow Cyt (Intra) and reacts with Mouse, Human, Rat, Synthetic peptide samples. Cited in 8 publications.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me3, H3K4me, H3K4

3 Images

Lab

Western blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade - BSA and Azide free (AB313500)

This data was developed using ab213224, the same antibody clone in a different buffer formulation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : Lane 1-4 : 5% NFDM/TBST Lane 5-12 : 2% BSA/TBST We recommend using 2% BSA as blocking and antibody dilution buffer.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-tri-methyl-k4-antibody-epr20551-225-chip-grade-ab213224'>ab213224</a>) at 1/1000 dilution

Lanes 1, 5 and 9:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lanes 2, 6 and 10:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 3, 7 and 11:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lanes 4, 8 and 12:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

false

Exposure time: 40s

Lab

Western blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade - BSA and Azide free (AB313500)

This data was developed using ab213224, the same antibody clone in a different buffer formulation.

We recommend to use 2% BSA/TBST as blocking and antibody dilution buffer.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

All lanes:

Lane 1:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 15 kDa,36 kDa

false

Exposure time: 40s

ChIC/CUT&RUN sequencing - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade - BSA and Azide free (AB313500)

ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade - BSA and Azide free (AB313500)

ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 2 µg of ab213224 [EPR20551-225]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The ChIP data was conducted on chromatin prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 4 µg of ab213224. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods. This data was developed using ab213224, the same antibody clone in a different buffer formulation.

Unconjugated

Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade
Mouse version

Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric)
Mouse version

Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - Ms IgG1 (Chimeric) – BSA and Azide Free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225]
578 PE

PE Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20551-225

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IP, Dot, CUT&Tag, WB, ChIP-seq, ChIC/CUT&RUN-seq, ChIP, Flow Cyt (Intra), ICC/IF, PepArr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We recommend to use 2% BSA as blocking and antibody dilution buffer.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "CUTTag" : {"fullname" : "CUT&Tag", "shortname":"CUT&Tag"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "CUTTag-species-checked": "guaranteed", "CUTTag-species-dilution-info": "", "CUTTag-species-notes": "", "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "We recommend to use 2% BSA as blocking and antibody dilution buffer.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Mouse": { "CUTTag-species-checked": "testedAndGuaranteed", "CUTTag-species-dilution-info": "", "CUTTag-species-notes": "", "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "We recommend to use 2% BSA as blocking and antibody dilution buffer.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "CUTTag-species-checked": "guaranteed", "CUTTag-species-dilution-info": "", "CUTTag-species-notes": "", "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "We recommend to use 2% BSA as blocking and antibody dilution buffer.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide": { "CUTTag-species-checked": "notRecommended", "CUTTag-species-dilution-info": "", "CUTTag-species-notes": "", "ChICCUTRUNseq-species-checked": "notRecommended", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 tri methyl K4

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Aging 12: PubMed33428595

2021

Long noncoding RNA TPT1-AS1 promotes the progression and metastasis of colorectal cancer by upregulating the TPT1-mediated FAK and JAK-STAT3 signalling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Leiyi Zhang,Fei Ye,Zhongkun Zuo,Ding Cao,Yu Peng,Zedong Li,Jiangsheng Huang,Lunxi Duan

Cancer research 80:2599-2611 PubMed32312832

2020

ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Zhang,Qi Huo,Chao Ge,Fangyu Zhao,Qingqing Zhou,Xiaoxia Chen,Hua Tian,Taoyang Chen,Haiyang Xie,Ying Cui,Ming Yao,Hong Li,Jinjun Li

International journal of molecular sciences 20: PubMed31615031

2019

The G9a Histone Methyltransferase Inhibitor BIX-01294 Modulates Gene Expression during Gametocyte Development and Transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Che Julius Ngwa,Meike Jutta Kiesow,Lindsey Marie Orchard,Afia Farrukh,Manuel Llinás,Gabriele Pradel

Diabetologia 62:2129-2142 PubMed31399844

2019

Dysregulated expression but redundant function of the long non-coding RNA HOTAIR in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Syamantak Majumder,Mitchell J Hadden,Karina Thieme,Sri N Batchu,Divya Niveditha,Shibasish Chowdhury,Veera Ganesh Yerra,Suzanne L Advani,Bridgit B Bowskill,Youan Liu,Hana Vakili,Tamadher A Alghamdi,Kathryn E White,Laurette Geldenhuys,Ferhan S Siddiqi,Andrew Advani

Nature communications 10:3319 PubMed31346162

2019

JMJD6 is a tumorigenic factor and therapeutic target in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Wong,Yuting Sun,Zhichao Xi,Giorgio Milazzo,Rebecca C Poulos,Christoph Bartenhagen,Jessica L Bell,Chelsea Mayoh,Nicholas Ho,Andrew E Tee,Xiaoqiong Chen,Yang Li,Roberto Ciaccio,Pei Y Liu,Chen C Jiang,Qing Lan,Nisitha Jayatilleke,Belamy B Cheung,Michelle Haber,Murray D Norris,Xu D Zhang,Glenn M Marshall,Jenny Y Wang,Stefan Hüttelmaier,Matthias Fischer,Jason W H Wong,Hongxi Xu,Giovanni Perini,Qihan Dong,Rani E George,Tao Liu

Journal of cellular and molecular medicine 23:6072-6084 PubMed31264792

2019

CRISPR/dCas9-mediated activation of multiple endogenous target genes directly converts human foreskin fibroblasts into Leydig-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Huang,Xiangyu Zou,Liang Zhong,Yanping Hou,Jin Zhou,Zhiyuan Zhang,Xiaoyu Xing,Jie Sun

EMBO reports 19: PubMed30389724

2018

KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Yang,Lishuang Song,Xuefei Liu,Lige Bai,Guangpeng Li

Nucleic acids research 46:1793-1809 PubMed29253179

2017

The interaction of lncRNA EZR-AS1 with SMYD3 maintains overexpression of EZR in ESCC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Dan Zhang,Guo-Wei Huang,Ying-Hua Xie,Jian-Zhong He,Jin-Cheng Guo,Xiu-E Xu,Lian-Di Liao,Yang-Min Xie,Yong-Mei Song,En-Min Li,Li-Yan Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade - BSA and Azide free

Western blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade - BSA and Azide free (AB313500)

Western blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade - BSA and Azide free (AB313500)

ChIC/CUT&RUN sequencing - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade - BSA and Azide free (AB313500)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (8)

Product promise