Mouse Recombinant Monoclonal H3 tri methyl K4 antibody. Suitable for ICC/IF and reacts with Human, Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric) (AB317791), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-tri-methyl-k4-antibody-epr20551-225-chip-grade-mouse-igg1-chimeric-ab317791--immunocytochemistry-immunofluorescence-img432084.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric) (AB317791)")
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric) (AB317791), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-tri-methyl-k4-antibody-epr20551-225-chip-grade-mouse-igg1-chimeric-ab317791--immunocytochemistry-immunofluorescence-img432083.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric) (AB317791)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Additional Targets
H3c1 tri methyl K4
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me3
Recommended products
Mouse Recombinant Monoclonal H3 tri methyl K4 antibody. Suitable for ICC/IF and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20551-225
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade ab213224). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric) (ab317791), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-tri-methyl-k4-antibody-epr20551-225-chip-grade-mouse-igg1-chimeric-ab317791--immunocytochemistry-immunofluorescence-img432084.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric) (ab317791)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric) (ab317791)
This image was produced using Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - Ms IgG1 (Chimeric) – BSA and Azide Free ab317801, the same antibody clone but in a different formulation.
Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - Ms IgG1 (Chimeric) – BSA and Azide Free ab317801 staining Histone H3 (tri methyl K4) in Neuro2a cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - Ms IgG1 (Chimeric) – BSA and Azide Free ab317801 at 5µg/ml and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric) (ab317791), expandable thumbnail](https://content.abcam.com/products/images/histone-h3-tri-methyl-k4-antibody-epr20551-225-chip-grade-mouse-igg1-chimeric-ab317791--immunocytochemistry-immunofluorescence-img432083.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric) (ab317791)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade – Mouse IgG1 (Chimeric) (ab317791)
This image was produced using Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - Ms IgG1 (Chimeric) – BSA and Azide Free ab317801, the same antibody clone but in a different formulation.
Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - Ms IgG1 (Chimeric) – BSA and Azide Free ab317801 staining Histone H3 (tri methyl K4) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - Ms IgG1 (Chimeric) – BSA and Azide Free ab317801 at 5µg/ml and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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