Rabbit Polyclonal H3 tri methyl K56 antibody. Suitable for Dot, WB, IF and reacts with Synthetic peptide - Human, Caenorhabditis elegans, Human samples. Immunogen corresponding to Synthetic Peptide within Human H3C1 tri methyl K56.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human H3C1 tri methyl K56. The exact immunogen used to generate this antibody is proprietary information. Database link P68431
Reactivity data
Select an application| Dot | WB | IF | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Caenorhabditis elegans | Expected | Tested | Expected |
| Synthetic peptide - Human | Tested | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K56me3
Recommended products
Rabbit Polyclonal H3 tri methyl K56 antibody. Suitable for Dot, WB, IF and reacts with Synthetic peptide - Human, Caenorhabditis elegans, Human samples. Immunogen corresponding to Synthetic Peptide within Human H3C1 tri methyl K56.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human H3C1 tri methyl K56. The exact immunogen used to generate this antibody is proprietary information. Database link P68431
- Purification technique
- Affinity purification Immunogen
- Specificity
A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
- Concentration
- Purification notes
Anti-Histone H3 [Trimethyl Lys56] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunofluorescence - Anti-Histone H3 (tri methyl K56) antibody (ab272162)
Immunofluorescence analysis of ab272162. HeLa (Human epithelial cell line from cervix adenocarcinoma) cells are shown during prophase. Primary antibody ab272162 was used at 1:100 dilution for 1 hour at room temperature. Secondary antibody, Dylight 488 antibody at 1:10,000 for 45 minutes at room temperature. Histone H3 [Trimethyl Lys56] localization is nuclear and chromosomal and it is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red). Fixation was 0.5% PFA. Antigen retrieval was not required.
Western blot - Anti-Histone H3 (tri methyl K56) antibody (ab272162)
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
Other band(s): None.
All lanes: Western blot - Anti-Histone H3 (tri methyl K56) antibody (ab272162) at 1 µg/mL
All lanes: Caenorhabditis elegans embryo lysate at 30 µg
SecondaryAll lanes: IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
Dot Blot - Anti-Histone H3 (tri methyl K56) antibody (ab272162)
Dot Blot analysis of ab272162.
Lane 1: Ac.
Lane 2: me1.
Lane 3: me2.
Lane 4: me3.
Lane 5: unmodified.
Load: 1, 10, and 100 picomoles of peptide. Primary antibody ab272162 was used at 1:40 for 45 minutes at 4°C. Secondary antibody Dylight™488 rabbit antibody at 1:10,000 for 45 minutes at room temperature. Block: 5% BLOTTO overnight at 4°C.
Immunofluorescence - Anti-Histone H3 (tri methyl K56) antibody (ab272162)
Immunofluorescence of ab272162. HeLa (Human epithelial cell line from cervix adenocarcinoma) cells are shown during telophase. Primary antibody ab272162 was at a 1:100 dilution for 1 hour at room temperature. Dylight 488 secondary antibody followed at 1:10,000 for 45 minutes at toom temperature. Histone H3 [Trimethyl Lys56] localization is nuclear and chromosomal. Histone H3 [Trimethyl Lys56] staining is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red). Fixation was 0.5% PFA and antigen retrieval was not required.
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