JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272162

Anti-Histone H3 (tri methyl K56) antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal H3 tri methyl K56 antibody. Suitable for Dot, WB, IF and reacts with Synthetic peptide - Human, Caenorhabditis elegans, Human samples. Immunogen corresponding to Synthetic Peptide within Human H3C1 tri methyl K56.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K56me3, H3K56me, H3K56

4 Images
Immunofluorescence - Anti-Histone H3 (tri methyl K56) antibody (AB272162)
  • IF

Supplier Data

Immunofluorescence - Anti-Histone H3 (tri methyl K56) antibody (AB272162)

Immunofluorescence analysis of ab272162. HeLa (Human epithelial cell line from cervix adenocarcinoma) cells are shown during prophase. Primary antibody ab272162 was used at 1 : 100 dilution for 1 hour at room temperature. Secondary antibody, Dylight 488 antibody at 1 : 10,000 for 45 minutes at room temperature. Histone H3 [Trimethyl Lys56] localization is nuclear and chromosomal and it is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red). Fixation was 0.5% PFA. Antigen retrieval was not required.

Immunofluorescence - Anti-Histone H3 (tri methyl K56) antibody (AB272162)
  • IF

Supplier Data

Immunofluorescence - Anti-Histone H3 (tri methyl K56) antibody (AB272162)

Immunofluorescence of ab272162. HeLa (Human epithelial cell line from cervix adenocarcinoma) cells are shown during telophase. Primary antibody ab272162 was at a 1 : 100 dilution for 1 hour at room temperature. Dylight 488 secondary antibody followed at 1 : 10,000 for 45 minutes at toom temperature. Histone H3 [Trimethyl Lys56] localization is nuclear and chromosomal. Histone H3 [Trimethyl Lys56] staining is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red). Fixation was 0.5% PFA and antigen retrieval was not required.

Western blot - Anti-Histone H3 (tri methyl K56) antibody (AB272162)
  • WB

Unknown

Western blot - Anti-Histone H3 (tri methyl K56) antibody (AB272162)

Block : 5% BLOTTO overnight at 4°C.

Predicted/Observed size : ~15 kDa.

Other band(s) : None.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K56) antibody (ab272162) at 1 µg/mL

All lanes:

Caenorhabditis elegans embryo lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Dot Blot - Anti-Histone H3 (tri methyl K56) antibody (AB272162)
  • Dot

Unknown

Dot Blot - Anti-Histone H3 (tri methyl K56) antibody (AB272162)

Dot Blot analysis of ab272162.

Lane 1 : Ac.

Lane 2 : me1.

Lane 3 : me2.

Lane 4 : me3.

Lane 5 : unmodified.

Load : 1, 10, and 100 picomoles of peptide. Primary antibody ab272162 was used at 1 : 40 for 45 minutes at 4°C. Secondary antibody Dylight™488 rabbit antibody at 1 : 10,000 for 45 minutes at room temperature. Block : 5% BLOTTO overnight at 4°C.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Caenorhabditis elegans

Applications

WB, Dot, IF

applications

Immunogen

Synthetic Peptide within Human H3C1 tri methyl K56. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB254336

Anti-MTF2 antibody [EPR23536-87] - ChIP Grade

RabMAb|Recombinant

Immunoprecipitation - Anti-MTF2 antibody [EPR23536-87] - ChIP Grade (AB254336)

  • 5
  • 2
Primary Antibodies

AB10158

Anti-Histone H4 antibody - ChIP Grade

BOND RX™ Validated

Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158)

  • 4
  • 21
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IF" : {"fullname" : "Immunofluorescence", "shortname":"IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "testedAndGuaranteed", "IF-species-dilution-info": "1/40", "IF-species-notes": "<p></p>" }, "Caenorhabditis elegans": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IF-species-checked": "guaranteed", "IF-species-dilution-info": "", "IF-species-notes": "" }, "Synthetic peptide - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "notRecommended", "IF-species-dilution-info": "", "IF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Anti-Histone H3 [Trimethyl Lys56] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 tri methyl K56
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com