AB272163

Anti-Histone H3 (tri methyl K79) antibody

Name: Anti-Histone H3 (tri methyl K79) antibody (ab272163) | Abcam
Brand: Abcam Limited
SKU: AB272163

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Rabbit Polyclonal H3 tri methyl K79 antibody. Suitable for ChIP, Dot, WB, ICC/IF and reacts with Human, Mouse, Caenorhabditis elegans, Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Human H3C1 tri methyl K79.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K79me3, H3K79me, H3K79

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K79) antibody (AB272163)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K79) antibody (AB272163)

Immunofluorescence analysis of 0.5% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells during prophase labeling Histone H3 (tri methyl K79) with ab272163 at 1/100 dilution for 1 hour at room temperature, followed by a Dylight 488 secondary antibody at 1/10000 for 45 minutes at room temperature. Histone H3 (tri methyl K79) is green. Nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).

ChIP - Anti-Histone H3 (tri methyl K79) antibody (AB272163)

ChIP

Supplier Data

ChIP - Anti-Histone H3 (tri methyl K79) antibody (AB272163)

Chromatin Immunoprecipitation using ab272163. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 μg of ab272163 and 20 μl of magnetic IgG beads per immunoprecipitation. A "no antibody" (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Supplier Data

Western blot - Anti-Histone H3 (tri methyl K79) antibody (AB272163)

All lanes:

Western blot - Anti-Histone H3 (tri methyl K79) antibody (ab272163) at 1 µg/mL

Lane 1:

HeLa human epithelial cell line from cervix adenocarcinoma) histone prep at 30 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) histone prep at 30 µg

Lane 3:

C.elegans embryo lysate at 30 µg

Secondary

All lanes:

IRDye800™ rabbit secondary antibody at 1/10000 dilution

Predicted band size: 15 kDa

false

Supplier Data

Dot Blot - Anti-Histone H3 (tri methyl K79) antibody (AB272163)

Dot Blot of ab272163.

Lane 1 : K79 unmodified.

Lane 2 : K79me1.

Lane 3 : K79me2.

Lane 4 : K79me3.

Load : 1, 10, and 100 picomoles of peptide. Primary antibody ab272163 was used at 1/1000 for 45 minutes at 4°C. Followed by the secondary antibody Dylight™488 rabbit at 1/10000 for 45 minutes at room temperature.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Caenorhabditis elegans

Applications

ICC/IF, WB, Dot, ChIP

applications

Immunogen

Synthetic Peptide within Human H3C1 tri methyl K79. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Anti-Histone H3 [Trimethyl Lys79] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 tri methyl K79

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (tri methyl K79) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K79) antibody (AB272163)

ChIP - Anti-Histone H3 (tri methyl K79) antibody (AB272163)

Western blot - Anti-Histone H3 (tri methyl K79) antibody (AB272163)

Dot Blot - Anti-Histone H3 (tri methyl K79) antibody (AB272163)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Product promise