Rabbit Polyclonal H3 tri methyl K79 antibody. Suitable for ChIP, WB and reacts with Human, Cow samples. Cited in 149 publications.
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- eLife 12:2023Opposing, spatially-determined epigenetic forces impose restrictions on stochastic olfactory receptor choice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizaveta V Bashkirova,Nell Klimpert,Kevin Monahan,Christine E Campbell,Jason Osinski,Longzhi Tan,Ira Schieren,Ariel Pourmorady,Beka Stecky,Gilad Barnea,Xiaoliang Sunney Xie,Ishmail Abdus-Saboor,Benjamin M Shykind,Bianca J Marlin,Richard M Gronostajski,Alexander Fleischmann,Stavros LomvardasPubMed 38108811
- Nature 622:619-6262023Inhibition of fatty acid oxidation enables heart regeneration in adult mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Li,Fan Wu,Stefan Günther,Mario Looso,Carsten Kuenne,Ting Zhang,Marion Wiesnet,Stephan Klatt,Sven Zukunft,Ingrid Fleming,Gernot Poschet,Astrid Wietelmann,Ann Atzberger,Michael Potente,Xuejun Yuan,Thomas BraunPubMed 37758950
- Cell death & disease 14:5252023Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Yang,Yuxi Ou,Quan Zhou,Yingchun Liang,Weijian Li,Yiling Chen,Wensun Chen,Siqi Wu,Yifan Chen,Xiyu Dai,Xinan Chen,Tian Chen,Shengming Jin,Yufei Liu,Limin Zhang,Shenghua Liu,Yun Hu,Lujia Zou,Shanhua Mao,Haowen JiangPubMed 37582769
- The FEBS journal 290:3539-35622023A screen for histone mutations that affect quiescence in S. cerevisiae.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEric M Small,Mary Ann OsleyPubMed 36871139
- International journal of molecular sciences 24:2023Neuronal Dot1l Activity Acts as a Mitochondrial Gene-Repressor Associated with Human Brain Aging via H3K79 Hypermethylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHendrikus J Van Heesbeen,Lars Von Oerthel,Paul M De Vries,Cindy M R J Wagemans,Marten P SmidtPubMed 36674903
- Scientific reports 12:215062022Loss of the Ash2l subunit of histone H3K4 methyltransferase complexes reduces chromatin accessibility at promoters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMirna Barsoum,Alexander T Stenzel,Agnieszka Bochyńska,Chao-Chung Kuo,Alexander Tsompanidis,Roksaneh Sayadi-Boroujeni,Philip Bussmann,Juliane Lüscher-Firzlaff,Ivan G Costa,Bernhard LüscherPubMed 36513698
- Pathogens (Basel, Switzerland) 11:2022[PRION] States Are Associated with Specific Histone H3 Post-Translational Modification Changes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamantha N Cobos,Chaim Janani,Gabriel Cruz,Navin Rana,Elizaveta Son,Rania Frederic,Jailene Paredes Casado,Maliha Khan,Seth A Bennett,Mariana P TorrentePubMed 36558770
- Open biology 12:2200662022Cis- and trans-regulation by histone H4 basic patch R17/R19 in metazoan development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuedi Zhang,Xiangyu Wu,Ju Peng,Angyang Sun,Yan Guo,Pengchong Fu,Guanjun GaoPubMed 36382370
- Cell & bioscience 12:1342022Inhibition of the cardiac fibroblast-enriched histone methyltransferase Dot1L prevents cardiac fibrosis and cardiac dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Xu,Jinghuan Wang,Fen Long,Wen Zhong,Haibi Su,Zhenghua Su,Xinhua LiuPubMed 35986422
- Nucleic acids research 50:7889-79052022Induction of senescence upon loss of the Ash2l core subunit of H3K4 methyltransferase complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnieszka Bochyńska,Alexander T Stenzel,Roksaneh Sayadi Boroujeni,Chao-Chung Kuo,Mirna Barsoum,Weili Liang,Philip Bussmann,Ivan G Costa,Juliane Lüscher-Firzlaff,Bernhard LüscherPubMed 35819198
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | WB | |
|---|---|---|
| Human | Tested | Expected |
| Rat | Predicted | Predicted |
| Cow | Expected | Tested |
| Plasmodium falciparum | Predicted | Predicted |
| Saccharomyces cerevisiae | Predicted | Predicted |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-4.00000 µg chromatin for 6 µg chromatin | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Saccharomyces cerevisiae, Plasmodium falciparum | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info 1 µg/mL | Notes Abcam recommends using BSA as the blocking agent. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Saccharomyces cerevisiae, Plasmodium falciparum | Dilution info - | Notes - |
Associated Products
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K79me3
Recommended products
Rabbit Polyclonal H3 tri methyl K79 antibody. Suitable for ChIP, WB and reacts with Human, Cow samples. Cited in 149 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Reacts with Tri-methyl K79 of histone H3. Cross-reactivity to di-methyl K79. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab208189.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
ChIP - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade (ab2621)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab2621 (blue), and 20 μl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR. Primers and probes are located in the first kb of the transcribed region.
Western blot - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade (ab2621)
All lanes: Western blot - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade (ab2621) at 1 µg/mL
Lanes 1 and 7: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
Lane 2: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (ab4558)
Lane 3: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K79) peptide (ab4555)
Lane 4: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K79) peptide (ab4556)
Lane 5: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K79) peptide (ab4557)
Lane 6: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 (di methyl K79) peptide (ab4560)
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
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