Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade

• ChIP

Unknown

ChIP - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade (AB2621)

Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab2621 (blue), and 20 μl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR. Primers and probes are located in the first kb of the transcribed region.

• WB

Project

Western blot - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade (AB2621)

All lanes:

Western blot - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade (ab2621) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/en-us/products/unavailable/human-histone-h3-unmodified-peptide-ab4558'>ab4558</a>)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K79) peptide (<a href='/en-us/products/unavailable/human-histone-h3-mono-methyl-k79-peptide-ab4555'>ab4555</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K79) peptide (<a href='/en-us/products/unavailable/human-histone-h3-di-methyl-k79-peptide-ab4556'>ab4556</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K79) peptide (<a href='/en-us/products/unavailable/human-histone-h3-tri-methyl-k79-peptide-ab4557'>ab4557</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 (di methyl K79) peptide (<a href='/en-us/products/unavailable/human-histone-h4-di-methyl-k79-peptide-ab4560'>ab4560</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

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