Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] - ChIP Grade

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] - ChIP Grade (AB208189)

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 4 μg of ab208189 [EPR17468(2)]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. Additional screenshots of mapped reads can be downloaded here.

• WB

Supplier Data

Western blot - Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] - ChIP Grade (AB208189)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] - ChIP Grade (ab208189) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

true

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] - ChIP Grade (AB208189)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] - ChIP Grade (ab208189) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

true

Exposure time: 8s

• PepArr

Unknown

Peptide Array - Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] - ChIP Grade (AB208189)

ab208189 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Product Protocol section.