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Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade is a rabbit polyclonal antibody that is used to detect Histone H3 in ChIP, ICC/IF, IHC-P, Western blot. Suitable for Cow, Human, Mouse samples.

- Tried and trusted by researchers since 2002


Images

ChIP - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (AB8898), expandable thumbnail
  • Western blot - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (AB8898), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (AB8898), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (AB8898), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (AB8898), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form
Liquid
Clonality
Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFChIPWB
Human
Expected
Tested
Tested
Expected
Mouse
Expected
Expected
Expected
Expected
Rat
Predicted
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Predicted
Cow
Expected
Expected
Expected
Tested
Cyanidioschyzon merolae
Predicted
Predicted
Predicted
Predicted
Drosophila melanogaster
Predicted
Predicted
Predicted
Predicted
Indian muntjac
Predicted
Predicted
Predicted
Predicted
Mammals
Predicted
Predicted
Predicted
Predicted
Saccharomyces cerevisiae
Predicted
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Predicted
Xenopus tropicalis
Predicted
Predicted
Predicted
Predicted

Expected
Expected

Species
Human
Dilution info
1/400
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/400
Notes

-

Species
Cow
Dilution info
1/400
Notes

-

Predicted
Predicted

Species
Rat, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Indian muntjac, Mammals, Xenopus tropicalis, Cyanidioschyzon merolae
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
0.5 µg/mL
Notes

-

Expected
Expected

Species
Cow
Dilution info
-
Notes

-

Species
Mouse
Dilution info
0.5 µg/mL
Notes

-

Predicted
Predicted

Species
Rat, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Indian muntjac, Mammals, Xenopus tropicalis, Cyanidioschyzon merolae
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
2.00000-4.00000 µg chromatin for 25 µg chromatin
Notes

We recommend SAT-alpha ChIP primer pair ab269263 as a positive control.

Expected
Expected

Species
Cow
Dilution info
-
Notes

-

Species
Mouse
Dilution info
2.00000-4.00000 µg chromatin for 25 µg chromatin
Notes

We recommend SAT-alpha ChIP primer pair ab269263 as a positive control.

Predicted
Predicted

Species
Rat, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Indian muntjac, Mammals, Xenopus tropicalis, Cyanidioschyzon merolae
Dilution info
-
Notes

-

Tested
Tested

Species
Cow
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat, Chicken, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Indian muntjac, Mammals, Xenopus tropicalis, Cyanidioschyzon merolae
Dilution info
-
Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

Function

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Recommended products

Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade is a rabbit polyclonal antibody that is used to detect Histone H3 in ChIP, ICC/IF, IHC-P, Western blot. Suitable for Cow, Human, Mouse samples.

- Tried and trusted by researchers since 2002

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique
Affinity purification Immunogen
Specificity

Histone H3 (tri methyl K9) antibody (ab8898) is specific for Histone H3 tri methyl Lysine 9. Shows slight cross-reactivity with tri methyl K27, which shares a similar epitope (please see Western blot image). Does not react with mono or di methylated K9. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab176916.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) is a rabbit polyclonal antibody and is validated for use in ChIP, ICC/IF, IHC-P and WB.

Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) was first used in a scientific publication in 2003 and has been cited over 1737 times in peer reviewed journals. It's performance in ChIP, Western Blot, IHC in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) has high sensitivity and specificity.

Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) has 91 independent reviews from customers.

Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) specifically detects Histone H3 Tri Methyl-K9 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 100 µg selling sizes.

Top cited antibody for this target with >2000 citations and >60 five star abreviews. Key target to study histone H3 trimethylation, epigenetic regulation and chromatin structure. This antibody is crucial in cancer research, particularly in understanding H3K9me3's role in gene silencing and heterochromatin formation and is widely used in studies of transcriptional repression and histone modifications involving histone methyltransferases. Key histone marker that has been implicated in both transcriptional activation and silencing.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Histone H3 is a core component of the nucleosome playing an important role in DNA packaging within eukaryotic cells. Often referred to as H3 it has a molecular weight of around 15 kDa. Histone H3 is expressed in the nucleus of nearly all eukaryotic cells where it interacts with DNA and other histone proteins to form chromatin structure. Scientists frequently study histone H3 using techniques like Western blotting and immunohistochemistry utilizing antibodies such as anti-histone or phospho-histone H3 to distinguish post-translational modifications.

Biological function summary

Histone H3 participates in chromatin remodeling and gene transcription regulation. As part of the nucleosome complex it influences DNA accessibility and subsequently gene expression. Through post-translational modifications including methylation and phosphorylation H3 modulates chromatin structure. Common modifications include mono- di- and tri-methylation at various lysine residues like K56 which affect transcriptional activation or repression. These modifications form a part of the histone code a framework for regulating chromatin dynamics and genetic information.

Pathways

Histone H3 modifications are integral to several signaling pathways that modulate gene expression and cellular responses. The histone code interpreted by other proteins such as transcription factors and chromatin remodelers contributes significantly to the regulation of the cell cycle and signal transduction pathways like the MAPK/ERK pathway. H3 interacts closely with regulatory proteins including other histones and transcription machinery to facilitate these complex processes.

Associated diseases and disorders

Histone H3 modifications are associated with various health conditions including cancer and neurological disorders. Aberrations in histone H3 methylation are linked to oncogenic transformations with disruptions often found in cancer types like glioblastoma and breast cancer. The alterations in H3 also connect to Alzheimer's disease through interactions with other histone modifications and their impact on gene expression patterns critical for neural function. Understanding these modifications helps provide insights into disease mechanisms and potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • ChIP - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898), expandable thumbnail

    ChIP - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898)

    Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab8898 (blue), and 20 μl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898), expandable thumbnail

    Western blot - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898)

    Lane 8 shows that Rabbit polyclonal to Histone H3 (tri methyl K9) is blocked by the addition of the immunizing peptide (Human Histone H3 (tri methyl K9) peptide ab1773). Cross-reactivity with Histone H3 peptide - tri methyl K27 (Human Histone H3 (tri methyl K27) peptide ab1782) is also shown in Lane 11.

    All lanes: Western blot - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) at 1 µg/mL

    All lanes: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 15 kDa

    Observed band size: 17 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898)

    ab8898 staining Histone H3 (tri methyl K9) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab8898 at 0.5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898)

    Histone H3 (tri methyl K9) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Histone H3 (tri methyl K9) antibody

    IHC image of ab8898 staining Histone H3 (tri methyl K9) in normal human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8898, 1/400 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898)

    ICC/IF image of ab8898 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8898, 0.1μg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

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