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Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade is a rabbit recombinant monoclonal antibody that is used to detect Histone H3 in CUT&Tag, ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Dot, ICC/IF, IHC-P, PepArr, Western blot. Suitable for Human, Mouse, Rat samples.

- Cited in over 50 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR16601 is cited in over 80 publications


Images

ChIC/CUT&RUN sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (AB176916), expandable thumbnail
  • ChIP - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (AB176916), expandable thumbnail
  • CUT&Tag sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (AB176916), expandable thumbnail
  • ChIP-sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (AB176916), expandable thumbnail
  • Western blot - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (AB176916), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
CUT&TagChIC/CUT&RUN-seqChIPDotWBPepArrICC/IFChIP-seqIHC-P
Human
Expected
Tested
Tested
Expected
Tested
Expected
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Tested
Tested
Expected
Tested
Tested
Tested
Rat
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
2 µg
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
2 µg for 25 µg chromatin
Notes

Use SAT-alpha ChIP primer pair ab269263 as positive control.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
4 µg for 107 Cells
Notes

-

Species
Human
Dilution info
4 µg for 107 Cells
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

Recommended products

Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade is a rabbit recombinant monoclonal antibody that is used to detect Histone H3 in CUT&Tag, ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Dot, ICC/IF, IHC-P, PepArr, Western blot. Suitable for Human, Mouse, Rat samples.

- Cited in over 50 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR16601 is cited in over 80 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16601
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in CUT&Tag, ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Dot, ICC/IF, IHC-P, PepArr, WB in human, mouse, rat samples.

Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) has been cited over 54 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) has 9 independent reviews from customers.

Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) specifically detects Histone H3 Tri Methyl-K9 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 uL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR16601 - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade - BSA and Azide free ab232324.

Antibody clone EPR16601 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, PE, APC, Alexa Fluor® 75 (Alexa Fluor® 488 Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade ab284635, Alexa Fluor® 647 Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade ab288339, ab32226, ab32227, ab32228, ab32229, Anti-RAP1GAP antibody [Y135] ab32119).

Histone H3 (tri methyl K9), also known as H3K9me3, is an important epigenetic modification associated with heterochromatin formation and gene silencing. This antibody is crucial in cancer research, particularly in understanding H3K9me3's role in gene silencing and heterochromatin formation, and is widely used in studies of transcriptional repression and histone modifications involving histone methyltransferases. Key histone marker that has been implicated in both transcriptional activation and silencing. The tri methyl K9 antibody is essential for studying this modification's role in transcription regulation, DNA repair, and chromosomal stability. Abnormal H3K9me3 levels are linked to various diseases, including cancer and neurological disorders. Monitoring H3K9me3 can provide valuable insights into disease mechanisms and potential therapeutic targets. Validated for CUT&Tag, CUT&RUN and ChIP-seq, which are key applications to map protein-DNA interactions on a genome-wide scale using NGS.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

  • ChIC/CUT&RUN sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ChIC/CUT&RUN pAG-MNase ab285373, 105 HeLa cells and 2μg of ab176916 [EPR16601]. The resulting DNA was sequenced on the NovaSeq 6000 (PE150).
    Additional screenshots of mapped reads can be downloaded here.

  • ChIP - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    ChIP - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab176916 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • CUT&Tag sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail
    This experiment and image is courtesy of Dr Marek Bartosovic, Gonçalo Castelo-Branco Group, Karolinska Institutet.

    CUT&Tag sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    CUT&Tag-seq was performed using 200,000 Oli-neu (Oligodendrocyte progenitor) cells. Cells were permeabilized with 0.05% Digitonin and 0.01% NP-40 for 3 minutes. A 1:100 dilution of Recombinant Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) was used, along with a Guinea pig anti-rabbit Secondary. DNA was seg using Illumina NovaSeq S Prime to a depth of 19 million reads.

    This image is courtesy of Dr Marek Bartosovic, Gonçalo Castelo-Branco Group, Karolinska Institutet.

  • ChIP-sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    ChIP-sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 4 μg of ab176916 [EPR16601]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
    Additional screenshots of mapped reads can be downloaded here.

  • Western blot - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    Western blot - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2: 4 seconds.

    All lanes: Western blot - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) at 1/1000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H3 (tri methyl K9) with ab176916 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on human colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Histone H3 (tri methyl K9) with ab176916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab176916 at 1/2000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Dot Blot - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    Dot Blot - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Dot blot analysis of Histone H3 (tri methyl K9) peptide (Lane 1), Histone H3K9 unmodified peptide (Lane 2), Histone H3 (crotonyl K4) peptide (Lane 3) and Histone H3K4 unmodified peptide (Lane 4) labeled using ab176916 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Peptide Array - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    Peptide Array - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    ab176916 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Histone H3 (tri methyl K9) with ab176916 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on mouse liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Histone H3 (tri methyl K9) with ab176916 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on rat liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (tri methyl K9) with ab176916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab176916 at 1/2000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • ChIC/CUT&RUN sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 2 µg of ab176916 [EPR16601]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    The ChIP data was conducted on chromatin prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 4 µg of ab176916. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
    Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • CUT&Tag - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail
    This experiment and image is courtesy of Dr Marek Bartosovic, Gonçalo Castelo-Branco Group, Karolinska Institutet.

    CUT&Tag - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    CUT&Tag-seq was performed using 200,000 Oli-neu (Oligodendrocyte progenitor) cells. Cells were permeabilized with 0.05% Digitonin and 0.01% NP-40 for 3 minutes. A 1:100 dilution of Recombinant Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) was used, along with a Guinea pig anti-rabbit Secondary. DNA was seg using Illumina NovaSeq S Prime to a depth of 19 million reads.
    This image is courtesy of Dr Marek Bartosovic, Gonçalo Castelo-Branco Group, Karolinska Institutet.
    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • Immunohistochemistry - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    Immunohistochemistry - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Immunohistochemical analysis of formalin fixed paraffin embedded human liver labelling Histone H3 (tri methyl K9) with ab176916 at a concentration of 0.07 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

    ab176916 Anti-Histone H3 (tri methyl K9) antibody [EPR16601] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

  • ChIP-sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916), expandable thumbnail

    ChIP-sequencing - Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916)

    Chromatin was prepared from Mouse Embryonic Fibroblasts cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 4 µg of Anti-Histone H3 (tri methyl K9) antibody (ab176916). ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

    Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com