AB238310

Anti-Histone H3 (unmodified K4) antibody [RM186]

Name: Anti-Histone H3 (unmodified K4) antibody [RM186] (ab238310) | Abcam
Brand: Abcam Limited
SKU: AB238310

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(1 Publication)

Rabbit Monoclonal H3 unmod K4 antibody. N-terminal. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Histone H3.1 unmod K4.

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H3 (unmodified K4) (red) using ab238310 at 2 μg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).``

Supplier Data

Western blot - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

All lanes:

Western blot - Anti-Histone H3 (unmodified K4) antibody [RM186] (ab238310) at 1 µg/mL

Lane 1:

Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate

Lane 2:

Acid extracts of HeLa cells treated with Nocodazole

Lane 3:

Acid extracts of HeLa cells

Lane 4:

Recombinant Histone H3.1

Predicted band size: 15 kDa

false

ELISA - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

ELISA

Supplier Data

ELISA - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

ab238310 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono-methylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.``

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM186

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, ELISA

applications

Immunogen

Synthetic Peptide within Human Histone H3.1 unmod K4. The exact immunogen used to generate this antibody is proprietary information.

P68431

Specificity

ab238310 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-2 µg/mL", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information Histone H3.1 unmod K4

Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Journal of cachexia, sarcopenia and muscle 16:e13734 PubMed39971710

2025

SIRT6 Ameliorates Cancer Cachexia-Associated Adipose Wasting by Suppressing TNFR2 Signalling in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Xu,Yida Wang,Fang Wang,Yannan Guo,Yu Ren,Vivien Low,Sungyun Cho,Qingfei Liu,Ying Qiu,Xue Li,Kang Yu,Zhongchi Li,Zhao Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3 (unmodified K4) antibody [RM186]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

Western blot - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

ELISA - Anti-Histone H3 (unmodified K4) antibody [RM186] (AB238310)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Alternative Names

Publications (1)

Product promise