Anti-Histone H3.3 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 antibody (AB62642)

Immunofluorescence analysis of HeLa cells, using ab62642 at a 1/500 dilution.
Left image untreated.
Right image treated with peptide.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody (AB62642)

Immunohistochemistry analysis of paraffin embedded human brain tissue using ab62642 at a 1/50 dilution.
Left image untreated.
Right image treated with peptide.

• WB

Unknown

Western blot - Anti-Histone H3.3 antibody (AB62642)

The 19 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Histone H3.3.

All lanes:

Western blot - Anti-Histone H3.3 antibody (ab62642) at 1 µg/mL

All lanes:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 19 kDa,45 kDa

true

Exposure time: 12min

• WB

CiteAb

Western blot - Anti-Histone H3.3 antibody (AB62642)

Histone H3.3 western blot using anti-Histone H3.3 antibody ab62642. Publication image and figure legend from Su, C. H., Cheng, C., et al., 2016, PLoS One, PubMed 27228173.

ab62642 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab62642 please see the product overview.

H2ac depletion induces telomere-repeat loss and telomere dysfunction.(A) Western blot of cell extracts prepared from control KD and H2ac KD cells using antibodies labeled on the right. (B) Telomere length analysis of MCF-7 and IMR-90 cells with control or H2ac siRNAs by telomere restriction fragment (TRF) analysis. MCF-7 and IMR-90 cells were harvested at day 5 after two separate transfections with control and H2ac siRNA. Telomere-repeat length and intensity was measured by restriction digest of genomic DNA with AluI/MboI and Southern hybridization with DIG-labeled (TTAGGG)4 probe (top panel). The GAPDH region was used as a control for DNA loading (bottom panel). The position of molecular weights (MWs; kb) is indicated on the left. (C) Telomere-ChIP assay indicates the effect of H2ac depletion on the occupancy of TRF1, TRF2 and POT1 at telomeres with telomere-specific sequences or Alu sequences using dot blot. Quantification of TTAGGG repeat DNA in the panel recovered in each ChIP is shown. The average of experiments performed in triplicate is shown. The P value was calculated using a Student's two-tailed t-test. (D) Effect of H2ac depletion on soluble and chromatin-bound TRF1, TRF2 and POT1 proteins. Equal cell equivalents of cytoplasmic proteins (CP), nucleoplasmic proteins (NP), and the chromatin-bound fraction (CB) were analyzed. α-tubulin is cytoplasmic and histone H3 is a chromatin protein.

false