Rabbit Recombinant Monoclonal H3 antibody. Carrier free. Suitable for ChIP, Dot, WB, ICC/IF, ChIP-seq, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![ChIP - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (AB208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--chip-img5571.jpg/_jcr_content/renditions/webp-475.webp "ChIP - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (AB208690)")
![ChIP-sequencing - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (AB208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--chip-sequencing-img115678.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (AB208690)")
![Dot Blot - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (AB208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--dot-blot-img5930.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (AB208690)")
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (AB208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--immunocytochemistry-immunofluorescence-img102662.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (AB208690)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (AB208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104479.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (AB208690)")
Publications
Filter by
- Nucleic acids research 51:1662-16732022PRC2-independent actions of H3.3K27M in embryonic stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLea R Z Cohen,Binyamin Kaffe,Eden Deri,Chen Leibson,Malka Nissim-Rafinia,Moria Maman,Nofar Harpaz,Guy Ron,Efrat Shema,Eran MeshorerPubMed 36156096
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 59% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | Dot | WB | ICC/IF | ChIP-seq | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Tested | Tested | Expected |
| Mouse | Expected | Tested | Expected | Expected | Expected | Expected |
| Rat | Expected | Expected | Expected | Expected | Expected | Tested |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg for 30 µg chromatin | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
2 products for Alternative Version
Target data
Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3.3A, H3F3, H3F3A, PP781, H3-3B, H3.3B, H3F3B, H3-3A, Histone H3.3
Recommended products
Rabbit Recombinant Monoclonal H3 antibody. Carrier free. Suitable for ChIP, Dot, WB, ICC/IF, ChIP-seq, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 59% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17899
- Purification technique
- Affinity purification Protein A
- Specificity
The immunogen sequence used for this antibody is 100% identical to the protein sequence shown for H3.X and H3.Y in Szenker et al., 2011 (PubMed ID 21263457). These H3.X and H3.Y protein sequences have not been reviewed by UniProt. The immunogen sequense is also 100% homologous to Histone H3.6. For further information on this antibody, please contact our technical support team.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab208690 is the carrier-free version of Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![ChIP - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--chip-img5571.jpg "ChIP - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)")
ChIP - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)
Chromatin was prepared from HeLa (Human epithelial cells from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840).
![ChIP-sequencing - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--chip-sequencing-img115678.jpg "ChIP-sequencing - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)")
ChIP-sequencing - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840).
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade (Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840). ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed by Active Motif, Carlsbad, CA.
Additional screenshots of mapped reads can be downloaded here.![Dot Blot - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--dot-blot-img5930.jpg "Dot Blot - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)")
Dot Blot - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)
Dot blot analysis of Histone H3.3 peptide (Lane 1), and Histone H3.1 peptide (Lane 2), labeled using Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840).
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--immunocytochemistry-immunofluorescence-img102662.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3.3 with Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104479.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H3.3 with Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129461.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Histone H3.3 with Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse stomach tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25173.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H3.3 with Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Rat colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade ab176840).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129461.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-antibody-epr17899-bsa-and-azide-free-ab208690--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25173.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)")
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