Rabbit Recombinant Monoclonal H3 mutated G34R antibody. Carrier free. Suitable for IHC-P, IP, ChIP, Dot, WB, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate - Human, Synthetic peptide - Human, Transfected cell line - Human samples.
Images
![ChIP - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (AB273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--chip-img103676.jpg/_jcr_content/renditions/webp-475.webp "ChIP - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (AB273635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (AB273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82860.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (AB273635)")
![Immunoprecipitation - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (AB273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--immunoprecipitation-img5749.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (AB273635)")
![Western blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (AB273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--western-blot-img20578.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (AB273635)")
![Flow Cytometry (Intracellular) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (AB273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--flow-cytometry-intracellular-img38491.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (AB273635)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | ChIP | Dot | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Expected | Expected | Expected | Expected |
| Synthetic peptide - Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Transfected cell line - Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Transfected cell lysate - Human | Not recommended | Not recommended | Tested | Tested | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Synthetic peptide - Human, Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Synthetic peptide - Human, Transfected cell line - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Transfected cell line - Human | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Transfected cell line - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Transfected cell line - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Transfected cell line - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
Histone H3.3, H3F3, H3F3B, H3.3B, H3-3B, PP781, H3F3A, H3.3A, H3-3A
Recommended products
Rabbit Recombinant Monoclonal H3 mutated G34R antibody. Carrier free. Suitable for IHC-P, IP, ChIP, Dot, WB, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate - Human, Synthetic peptide - Human, Transfected cell line - Human samples.
Alternative names
Histone H3.3, H3F3, H3F3B, H3.3B, H3-3B, PP781, H3F3A, H3.3A, H3-3A
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR23519-91
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab273635 is the carrier-free version of Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![ChIP - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--chip-img103676.jpg "ChIP - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)")
ChIP - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)
Chromatin was prepared from 293T transfacted with Histone H3.3(mutated G34 R) and 293T transfacted with Histone H3.3(WT)
cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 2 μg of Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 (red), or 2 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 25 μl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82860.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)
Immunohistochemical analysis of paraffin-embedded Human giant cell tumor of bone tissue labeling Histone H3.3 (mutated G34 R) with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on human giant cell tumor of bone (PMID: 29241742). The section was incubated with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402).
![Immunoprecipitation - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--immunoprecipitation-img5749.jpg "Immunoprecipitation - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)")
Immunoprecipitation - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)
Histone H3.3 (mutated G34 R) was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) (transfected with Histone H3.3 G34R expression vector containing a myc-His-tag®) whole cell lysate with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34R expression vector containing a myc-His-tag®, whole cell lysate 10 ug
Lane 2: Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 IP in HEK-293T transfected with Histone H3.3 G34R expression vector containing a myc-His-tag® whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 in HEK-293T transfected with Histone H3.3 G34R expression vector containing a myc-His-tag® whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402).
All lanes: Immunoprecipitation - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade (Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402)
Predicted band size: 15 kDa
Observed band size: 20 kDa
![Western blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--western-blot-img20578.jpg "Western blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)")
Western blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 26 seconds .
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402).
All lanes: Western blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade (Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34R expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with Histone H3.3 (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 3: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34V expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 4: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34W expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 15 kDa
Observed band size: 20 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 26 seconds.
![Flow Cytometry (Intracellular) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--flow-cytometry-intracellular-img38491.jpg "Flow Cytometry (Intracellular) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)")
Flow Cytometry (Intracellular) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with myc-tagged Histone H3.3 WT expression vector) (Left) or myc-tagged Histone H3.3 G34R expression vector (Right) cells labelling Histone H3.3 (mutated G34 R) with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 at 1/5000 dilution (0.01ug) (Both panels). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402).
![Dot Blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--dot-blot-img19779.jpg "Dot Blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)")
Dot Blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)
Dot blot analysis of Histone H3.3 (mutated G34 R) labeled with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 at 1/1000 dilution.
Lane 1: Histone H3.3 H3G34R peptide (aa28-40).
Lane 2: Histone H3.3 H3G34R peptide (aa26-36).
Lane 3: Histone H3.3 WT peptide (aa26-40).Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120023.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Histone H3.3 (mutated G34 R) with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human testis. The section was incubated with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402).
![Dot Blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--dot-blot-img19779.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-mutated-g34r-antibody-epr23519-91-chip-grade-bsa-and-azide-free-ab273635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120023.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade – BSA and Azide free (ab273635)")
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