AB92628

Anti-Histone H3.3 (phospho S31) antibody [EPR1873]

Name: Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (ab92628) | Abcam
Brand: Abcam Limited
SKU: AB92628
Price: 550 USD
Availability: InStock
Rating: 3 (4 reviews)

RabMAb
Recombinant
What is this?

(4 Reviews)

(18 Publications)

Rabbit Recombinant Monoclonal H3 phospho S31 antibody. Suitable for IHC-P, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples. Cited in 18 publications.

View Alternative Names

H3.3A, H3F3, H3F3A, PP781, H3-3B, H3.3B, H3F3B, H3-3A, Histone H3.3, H3S31p

7 Images

Flow Cytometry (Intracellular) - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Intracellular Flow Cytometry analysis of Hela (human cervix adenocarcinoma) cells treated with 100 ng/ml Nocodazole for 18 hours, labeling Histone H3.3 with purified ab92628 at 1/150 dilution (10μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluorr^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. Untreated control - Hela (human cervix adenocarcinoma) cells untreated with 100 ng/ml Nocodazole for 18 hours (Green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Paraffin-embedded human cervical carcinoma labelled with ab92628 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, LP treated and non-treated, labeling anti-Histone H3.3 (phospho S31) with ab92628 at 1/500 dilution followed by Goat anti-Rabbit secondary IgG Alexa Fluor^® 488 (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on M phase of HeLa cells, then the signal decreased after LP treatment.

For the pan antibody, there was no great difference after LP treatment. The data showed mostly nuclear staining.

Supplier Data

Western blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Blocking/dilution buffer : 2% BSA/TBST

All lanes:

Western blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (ab92628) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

Whole cell lysate from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with 100ng/ml of nocodazole for 18 hours at 15 µg

Lane 3:

Whole cell lysate from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with 100ng/ml of nocodazole for 18 hours. Membrane incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 15 kDa

false

Exposure time: 15s

Unknown

Western blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

All lanes:

Western blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (ab92628) at 1/1000 dilution

Lane 1:

HeLa cell lysate untreated at 10 µg

Lane 2:

HeLa cell lysate treated with Calyculin A at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 15 kDa

false

AbReview30108****

Western blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Representative western blot detecting Histone H3.3 (phospho S31) using ab92628 at 1/3000 dilution.

Recombinant Histone H3.3 protein was treated with recombinant kinase and 200 μM ATP for the indicated times. 0.2 μg of protein was loaded to each lane and phosphorylated Histone H3.3 was detected using ab92628. An HRP-conjugated goat anti-rabbit polyclonal (1/20000) was used as the secondary antibody

All lanes:

Western blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (ab92628)

Predicted band size: 15 kDa

false

This image is courtesy of an anonymous Abreview

Unknown

Dot Blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Dot Blot analysis of Lane 1 : Histone H3.3 (pS31) phospho peptide and Lane 2 : Histone H3.3 non-phospho peptide labeling Histone H3.3 (phospho S31) with ab92628 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer.

ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time : 10 seconds.

Carrier free

Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Histone H3.3 (phospho S31) antibody [EPR1873]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Histone H3.3 (phospho S31) antibody [EPR1873]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1873

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P, Dot, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3-3A pS31

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Laboratory animal research 41:25 PubMed40993770

2025

Phosphorylation-mimicking histone H3.3 rescues exercise-induced gene responses in an epigenetic aging model of mouse skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Sho Maruyama,Fuminori Kawano

Biochemical genetics 63:5394-5414 PubMed39656403

2024

Inflammatory Signaling Induces Mitochondrial Dysfunction and Neuronal Death in Traumatic Brain Injury via Downregulation of OXPHOS Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Dong,Hui Zhang,Lei Cai,Quanyi Ye,Heping Wang,Bo Liu,Wenhu Zhang,Junxin Li

Protein science : a publication of the Protein Society 33:e5126 PubMed39073155

2024

Haspin mediates H3.3S31 phosphorylation downstream of Aurora B in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Li,Meixian Wu,Yang Liu,Lihua Sun,Peiqiang Mu,Binbin Ma,Jing Xie

Nucleic acids research 51:10194-10217 PubMed37638746

2023

HIRA-mediated loading of histone variant H3.3 controls androgen-induced transcription by regulation of AR/BRD4 complex assembly at enhancers.

Applications

Unspecified application

Species

Unspecified reactive species

Viacheslav M Morozov,Alberto Riva,Sadia Sarwar,Wan-Ju Kim,Jianping Li,Lei Zhou,Jonathan D Licht,Yehia Daaka,Alexander M Ishov

Genesis (New York, N.Y. : 2000) 61:e23507 PubMed36656301

2023

A knockout-first model of H3f3a gene targeting leads to developmental lethality.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly Bush,Vanessa Cervantes,Jennifer Q Yee,Rachel H Klein,Paul S Knoepfler

Cellular and molecular gastroenterology and hepatology 14:527-551 PubMed35643233

2022

HIRA Supports Hepatitis B Virus Minichromosome Establishment and Transcriptional Activity in Infected Hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Maëlle Locatelli,Jean-Pierre Quivy,Fleur Chapus,Maud Michelet,Judith Fresquet,Sarah Maadadi,Amel Neila Aberkane,Audrey Diederichs,Julie Lucifora,Michel Rivoire,Geneviève Almouzni,Barbara Testoni,Fabien Zoulim

PLoS genetics 17:e1009868 PubMed34752469

2021

The H3.3K27M oncohistone affects replication stress outcome and provokes genomic instability in pediatric glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Irena Bočkaj,Tosca E I Martini,Eduardo S de Camargo Magalhães,Petra L Bakker,Tiny G J Meeuwsen-de Boer,Inna Armandari,Saskia L Meuleman,Marin T Mondria,Colin Stok,Yannick P Kok,Bjorn Bakker,René Wardenaar,Jonas Seiler,Mathilde J C Broekhuis,Hilda van den Bos,Diana C J Spierings,Femke C A Ringnalda,Hans Clevers,Ulrich Schüller,Marcel A T M van Vugt,Floris Foijer,Sophia W M Bruggeman

Communications biology 3:363 PubMed32647372

2020

Reciprocal H3.3 gene editing identifies K27M and G34R mechanisms in pediatric glioma including NOTCH signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kuang-Yui Chen,Kelly Bush,Rachel Herndon Klein,Vanessa Cervantes,Nichole Lewis,Aasim Naqvi,Angel M Carcaboso,Mirna Lechpammer,Paul S Knoepfler

Neoplasia (New York, N.Y.) 22:376-389 PubMed32629178

2020

BRAF inhibition in melanoma is associated with the dysregulation of histone methylation and histone methyltransferases.

Applications

Unspecified application

Species

Unspecified reactive species

Florina Grigore,Hana Yang,Nicholas D Hanson,Matthew W VanBrocklin,Aaron L Sarver,James P Robinson

Nature genetics 51:941-946 PubMed31152160

2019

Phosphorylation of histone H3.3 at serine 31 promotes p300 activity and enhancer acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Martire,Aishwarya A Gogate,Amanda Whitmill,Amanuel Tafessu,Jennifer Nguyen,Yu-Ching Teng,Melodi Tastemel,Laura A Banaszynski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H3.3 (phospho S31) antibody [EPR1873]

Flow Cytometry (Intracellular) - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Western blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Western blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Western blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Dot Blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (AB92628)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (18)

Product promise