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AB275929

Anti-Histone H3.3 (tri methyl K79) antibody [RM157]

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(1 Publication)

Rabbit Recombinant Monoclonal H3 tri methyl K79 antibody. Suitable for WB, Dot, PepArr and reacts with Human, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H3-3A tri methyl K79.

View Alternative Names

H3.3A, H3F3, H3F3A, PP781, H3-3B, H3.3B, H3F3B, H3-3A, Histone H3.3, H3K79me3, H3K79me, H3K79

3 Images
Western blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929)
  • WB

Supplier Data

Western blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929)

All lanes:

Western blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929) at 0.5 µg/mL

Lane 1:

Recombinant histone H3.3

Lane 2:

Acid extracts of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells

Predicted band size: 15 kDa

false

Peptide Array - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929)
  • PepArr

Supplier Data

Peptide Array - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929)

ab275929 specifically reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with other methylated lysines in Histone H3.

Dot Blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929)
  • Dot

Supplier Data

Dot Blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929)

A Peptide dot blot shows ab275929 only reacts to Histone H3 trimethyl-Lysine 79 (K79me3). No cross reactivity. with non-modified Lysine 79 (H3K79Ctrl), monomethylated. Lysine 79 (K79me1), or dimethylated Lysine 79 (K79me2).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM157

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, WB, PepArr

applications

Immunogen

Synthetic Peptide within Human H3-3A tri methyl K79. The exact immunogen used to generate this antibody is proprietary information.

P84243

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-1 µg/mL", "WB-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3-3A tri methyl K79
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:113866 PubMed38416638

2024

Atypical cofilin signaling drives dendritic cell migration through the extracellular matrix via nuclear deformation.

Applications

Unspecified application

Species

Unspecified reactive species

Harry Warner,Giulia Franciosa,Guus van der Borg,Britt Coenen,Felix Faas,Claire Koenig,Rinse de Boer,René Classens,Sjors Maassen,Maksim V Baranov,Shweta Mahajan,Deepti Dabral,Frans Bianchi,Niek van Hilten,Herre Jelger Risselada,Wouter H Roos,Jesper Velgaard Olsen,Laia Querol Cano,Geert van den Bogaart
View all publications
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Product promise

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