Rabbit Recombinant Monoclonal H3 tri methyl K79 antibody. Suitable for WB, Dot, PepArr and reacts with Human, Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Human H3-3A tri methyl K79.
Images
![Western blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-tri-methyl-k79-antibody-rm157-ab275929--western-blot-img157947.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929)")
![Dot Blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-tri-methyl-k79-antibody-rm157-ab275929--dot-blot-img100.png/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929)")
![Peptide Array - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-tri-methyl-k79-antibody-rm157-ab275929--peptide-array-img891.png/_jcr_content/renditions/webp-475.webp "Peptide Array - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (AB275929)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48.01% PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human H3-3A tri methyl K79. The exact immunogen used to generate this antibody is proprietary information. Database link P84243
Reactivity data
Select an application| WB | Dot | PepArr | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Synthetic peptide | Not recommended | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.20000-1.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PepArr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
Histone H3.3, H3F3B, H3.3B, H3-3B, PP781, H3F3A, H3F3, H3.3A, H3-3A, H3K79me3
Recommended products
Rabbit Recombinant Monoclonal H3 tri methyl K79 antibody. Suitable for WB, Dot, PepArr and reacts with Human, Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Human H3-3A tri methyl K79.
Alternative names
Histone H3.3, H3F3B, H3.3B, H3-3B, PP781, H3F3A, H3F3, H3.3A, H3-3A, H3K79me3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48.01% PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human H3-3A tri methyl K79. The exact immunogen used to generate this antibody is proprietary information. Database link P84243
- Clone number
RM157
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-tri-methyl-k79-antibody-rm157-ab275929--western-blot-img157947.png "Western blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)")
Western blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)
All lanes: Western blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929) at 0.5 µg/mL
Lane 1: Recombinant histone H3.3
Lane 2: Acid extracts of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells
Predicted band size: 15 kDa
![Dot Blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-tri-methyl-k79-antibody-rm157-ab275929--dot-blot-img100.png "Dot Blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)")
Dot Blot - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)
A Peptide dot blot shows ab275929 only reacts to Histone H3 trimethyl-Lysine 79 (K79me3). No cross reactivity. with non-modified Lysine 79 (H3K79Ctrl), monomethylated. Lysine 79 (K79me1), or dimethylated Lysine 79 (K79me2).
![Peptide Array - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929), expandable thumbnail](https://content.abcam.com/products/images/histone-h33-tri-methyl-k79-antibody-rm157-ab275929--peptide-array-img891.png "Peptide Array - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)")
Peptide Array - Anti-Histone H3.3 (tri methyl K79) antibody [RM157] (ab275929)
ab275929 specifically reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with other methylated lysines in Histone H3.
Downloads
Product protocols
- Visit the general protocols
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