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AB177028

Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal H3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

H3F3C, H3-5, Histone H3.3C, Histone H3.5

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human ovary tissue using ab150417 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab150417 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Nuclear staining of Mouse kidney using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Nuclear staining of Rat Lung tissue using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Histone H3.3C with ab150417 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Histone H3.3C with ab150417 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Nuclear astaining of Mouse liver using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Nuclear staining of Rat colon usign ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Nuclear staining of Mouse testis using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Thyroid gland tissue using ab150417 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Nuclear staining of Rat Brain using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

Nuclear staining of Mouse collon using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)
  • WB

Unknown

Western blot - Anti-Histone H3.3C antibody [EPR10085(B)] - BSA and Azide free (AB177028)

This data was developed using ab150417, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Histone H3.3C antibody [EPR10085(B)] (<a href='/en-us/products/primary-antibodies/histone-h33c-antibody-epr10085b-ab150417'>ab150417</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human testis lysate at 10 µg

Lane 3:

SH-SY5Y lysate at 10 µg

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10085(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab177028 is the carrier-free version of ab150417.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Hominid-specific H3.5/H3F3C preferentially colocalizes with euchromatin, and it is associated with actively transcribed genes.
See full target information H3-5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com