Anti-Histone H4 (2-hydroxyisobutyryl K8) antibody

• ICC

Supplier Data

Immunocytochemistry - Anti-Histone H4 (2-hydroxyisobutyryl K8) antibody (AB241250)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H4 (2-hydroxyisobutyryl K8) with ab241250 at 1/15 dilution in ICC.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (2-hydroxyisobutyryl K8) antibody (AB241250)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H4 (2-hydroxyisobutyryl K8) with ab241250 at 1/7.5 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor^®488-conjugated Goat anti-Rabbit IgG (H+L).

• WB

Supplier Data

Western blot - Anti-Histone H4 (2-hydroxyisobutyryl K8) antibody (AB241250)

All lanes:

Western blot - Anti-Histone H4 (2-hydroxyisobutyryl K8) antibody (ab241250) at 1/100 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours

Lane 2:

A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours

Lane 3:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours

Lane 4:

HEK-293 whole cell lysate, untreated (-)

Lane 5:

A549 whole cell lysate, untreated (-)

Lane 6:

K562 whole cell lysate, untreated (-)

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 11 kDa

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