AB61238

Anti-Histone H4 (acetyl K12) antibody

Name: Anti-Histone H4 (acetyl K12) antibody (ab61238) | Abcam
Brand: Abcam Limited
SKU: AB61238
Price: 545 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(23 Publications)

Rabbit Polyclonal Histone H4 acetyl L12 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, African green monkey, Drosophila melanogaster, Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human H4C1 acetyl L12 aa 1-50.

View Alternative Names

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4L12ac

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody (AB61238)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody (AB61238)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H4 (acetyl K12) antibody (ab61238) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of acetylated peptide.

Supplier Data

Western blot - Anti-Histone H4 (acetyl K12) antibody (AB61238)

All lanes:

Western blot - Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution

Lane 1:

HEK-293 cells (with TSA 400nM 24h)

Lane 2:

HEK-293 cells (without TSA 400nM 24h)

Predicted band size: 11 kDa

false

Supplier Data

Western blot - Anti-Histone H4 (acetyl K12) antibody (AB61238)

All lanes:

Western blot - Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution

All lanes:

KB cells

Predicted band size: 11 kDa

false

Unknown

Western blot - Anti-Histone H4 (acetyl K12) antibody (AB61238)

All lanes:

Western blot - Anti-Histone H4 (acetyl K12) antibody (ab61238) at 1/500 dilution

Lane 1:

Extracts from COS7 cells, treated with TSA (400nM, 24hours) with no acetylated peptide

Lane 2:

Extracts from COS7 cells, treated with TSA (400nM, 24hours) with acetylated peptide

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, African green monkey, Drosophila melanogaster, Mouse

Applications

WB, IHC-P, ELISA, ICC/IF

applications

Immunogen

Synthetic Peptide within Human H4C1 acetyl L12 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P62805

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H4C1 acetyl L12

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 10: PubMed34206992

2021

New Insights on NETosis Induced by : Dependence on ROS from Amoebas and Extracellular MPO Activity.

Applications

Unspecified application

Species

Unspecified reactive species

César Díaz-Godínez,Joshue Fabián Jorge-Rosas,Mario Néquiz,Santiago Martínez-Calvillo,Juan P Laclette,Carlos Rosales,Julio C Carrero

Aging 13:8849-8864 PubMed33742608

2021

HDAC11 inhibition disrupts porcine oocyte meiosis via regulating α-tubulin acetylation and histone modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Huang,Liyan Sui,Cong Fu,Yanhui Zhai,Xiangpeng Dai,Sheng Zhang,Ziyi Li

Scientific reports 9:17973 PubMed31784689

2019

Hat1 acetylates histone H4 and modulates the transcriptional program in Drosophila embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Júlia Varga,Szabina Korbai,Alexandra Neller,Nóra Zsindely,László Bodai

Alcoholism, clinical and experimental research 43:1145-1162 PubMed31074890

2019

Global DNA Methylation and Histone Posttranslational Modifications in Human and Nonhuman Primate Brain in Association with Prenatal Alcohol Exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica S Jarmasz,Hannah Stirton,Duaa Basalah,James R Davie,Sterling K Clarren,Susan J Astley,Marc R Del Bigio

Behavioural brain research 366:36-44 PubMed30880220

2019

Social isolation and social support at adulthood affect epigenetic mechanisms, brain-derived neurotrophic factor levels and behavior of chronically stressed rats.

Applications

Unspecified application

Species

Unspecified reactive species

Juliano Viana Borges,Betânia Souza de Freitas,Vinicius Antoniazzi,Cristophod de Souza Dos Santos,Kelem Vedovelli,Vivian Naziaseno Pires,Leticia Paludo,Maria Noêmia Martins de Lima,Elke Bromberg

Histopathology 74:227-238 PubMed30007038

2018

Prognostic value of histone H3 serine 10 phosphorylation and histone H4 lysine 12 acetylation in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Campos-Fernández,Flavia S Matsuo,Marília F Andrade,João P S Servato,Adriano M Loyola,Sérgio V Cardoso,Sindeval J Siva,Alberto da S Moraes,Paulo R de Faria

Frontiers in cellular and infection microbiology 8:226 PubMed30023352

2018

Induce Signaling via Raf/MEK/ERK for Neutrophil Extracellular Trap (NET) Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Zayda Fonseca,César Díaz-Godínez,Nancy Mora,Omar R Alemán,Eileen Uribe-Querol,Julio C Carrero,Carlos Rosales

Pathology, research and practice 214:876-880 PubMed29699903

2018

Evaluation of specific modified histones in lip carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Silas Antonio Juvencio de Freitas Filho,João Paulo Silva Servato,Rodrigo Tavares de Sá,Carla Silva Siqueira,Paulo Rogério de Faria,Adriano Mota Loyola,Sérgio Vitorino Cardoso

Environmental and molecular mutagenesis 59:375-385 PubMed29569274

2018

The effects of acetaldehyde exposure on histone modifications and chromatin structure in human lung bronchial epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Danqi Chen,Lei Fang,Hongjie Li,Chunyuan Jin

Environmental health perspectives 125:097019 PubMed28937961

2017

Regulation of Chromatin Assembly and Cell Transformation by Formaldehyde Exposure in Human Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Danqi Chen,Lei Fang,Shenglin Mei,Hongjie Li,Xia Xu,Thomas L Des Marais,Kun Lu,X Shirley Liu,Chunyuan Jin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H4 (acetyl K12) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody (AB61238)

Western blot - Anti-Histone H4 (acetyl K12) antibody (AB61238)

Western blot - Anti-Histone H4 (acetyl K12) antibody (AB61238)

Western blot - Anti-Histone H4 (acetyl K12) antibody (AB61238)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (23)

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