AB109463

Anti-Histone H4 (acetyl K16) antibody [EPR1004]

Name: Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) | Abcam
Brand: Abcam Limited
SKU: AB109463
Price: 400 USD
Availability: InStock
Rating: 5 (14 reviews)

RabMAb
Advanced Validation
Recombinant
What is this?

(14 Reviews)

(117 Publications)

Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) is a rabbit monoclonal antibody detecting Histone H4 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 80 publications

View Alternative Names

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4K16ac

22 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Histone H4 (acetyl K16) with purified ab109463 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunocytochemistry/ Immunofluorescence analysis of untreated HeLa cells (top row) and HeLa+ TSA(500ng/ml, 4h) cells (middle row) labeling Histone H4 (acetyl K16) with ab109463 at 1/500. Goat anti rabbit IgG(Alexa Fluor^® 488); ab150077 at 1/1000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI (blue) was used as a nuclear counterstain.

Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Intracellular Flow Cytometry analysis of HeLa cells labelling Histone H4 (acetyl K16)with unpurified ab109463 (red) at 1/130. Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/150). A rabbit monoclonal IgG was used as the isotype control (green).

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Intracellular Flow Cytometry analysis of HeLa cells labelling Histone H4 (acetyl K16) with purified ab109463 (red) at 1/200. Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/150). A rabbit monoclonal IgG was used as the isotype control (green).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells treated with TSA labelling Histone H4 (acetyl K16) with unpurified ab109463 (red) at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/100) and secondary antibody ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Histone H4 with unpurified ab109463 at 1/100.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Histone H4 (acetyl K16) with unpurified ab109463 (red) at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/100) and secondary antibody ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma labelling Histone H4 (acetly K16) with unpurified ab109463 at 1/100.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Overlay histogram showing HeLa cells stained with unpurified ab109463 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109463, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr^® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells treated with TSA labelling Histone H4 (acetyl K16) with purified ab109463 (red) at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/150) and secondary antibody ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Histone H4 (acetyl K16) with purified ab109463 (red) at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/150) and secondary antibody ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Histone H4 (acetyl K16) with unpurified ab109463 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Lab

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1500 dilution

All lanes:

HeLa cell lysate - treated with TSA at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Lab

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1000 dilution

All lanes:

HeLa cell lysate - treated with TSA at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Unknown

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

All lanes:

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1000 dilution

Lane 1:

HeLa cell lysates, untreated at 10 µg

Lane 2:

HeLa cell lysates treated with TSA at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

ChIC/CUT&RUN sequencing - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 2 µg of ab109463 [EPR1004]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ab285373 105 HeLa cells and 2μg of ab109463 [EPR1004]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. Additional screenshots of mapped reads can be downloaded here.

Lab

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1000 dilution

All lanes:

Mouse spleen tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L)

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Lab

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Blocking/Diluting buffer and concentration 5% NFDM/TBST

Lanes 1 - 2:

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/6000 dilution

Lanes 3 - 4:

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/24000 dilution

Lanes 1 and 3:

Untreated C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lanes 2 and 4:

C6 (Rat glial tumor glial cell) treated with Trichostatin A (final concentration is 500ng/ml) for 4 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1500 dilution

All lanes:

C6 cell lysate - treated with TSA at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Lab

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1500 dilution

All lanes:

Mouse spleen tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Lab

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1000 dilution

All lanes:

C6 cell lysate - treated with TSA at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

HRP

HRP Anti-Histone H4 (acetyl K16) antibody [EPR1004]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Histone H4 (acetyl K16) antibody [EPR1004]
Carrier free

Anti-Histone H4 (acetyl K16) antibody [EPR1004] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Histone H4 (acetyl K16) antibody [EPR1004]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1004

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), ChIC/CUT&RUN-seq, ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects Histone H4 acetylated on Lysine 16.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "2 µg", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/200", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Histone H4?
Anti-Histone H4 (acetyl K16) [EPR1004] (ab109463) specifically detects a band for Histone H4 (UniProt: P62805) at a molecular weight of 11kDa.

Trusted by the scientific community
Anti-Histone H4 (acetyl K16) [EPR1004] (ab109463) was first used in a scientific publication in 2011 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H4 (acetyl K16) [EPR1004] (ab109463) has over 10 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR1004] also available for your convenience: ab109463, Carrier free - ab194352, HRP - ab200859, Alexa Fluor® 488 - ab246771, Alexa Fluor® 647 - ab246772

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H4C1 acetyl K16

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Publications (117)

Recent publications for all applications. Explore the full list and refine your search

aBIOTECH 6:411-423 PubMed40994437

2025

Epigenetically poised chromatin states regulate and genes in soybean.

Applications

Unspecified application

Species

Unspecified reactive species

Linzhe Jin,Yihan Zhang,Jiayuan Guo,Xuexia Liu,Yanling Lai,Xinfang Huang,Yuhan Zou,Shichuang Yan,Xianzhe Dai,Zhenhui Zhong

Nucleic acids research 53: PubMed40985775

2025

Mammalian H4K16ac regulates the spatiotemporal order of genome replication rather than gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Milan,Valeria Runfola,Manthan Patel,Lucia Falbo,Roberta Noberini,Chiara Soriani,Simona Rodighiero,Tiziana Bonaldi,Vincenzo Costanzo,Madapura M Pradeepa,Paola Scaffidi

Nature cell biology 27:1482-1495 PubMed40921734

2025

Hbo1 and Msl complexes preserve differential compaction and H3K27me3 marking of active and inactive X chromosomes during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dounia Djeghloul,Sherry Cheriyamkunnel,Bhavik Patel,Holger Kramer,Alex Montoya,Karen E Brown,Chad Whilding,Tatyana B Nesterova,Guifeng Wei,Neil Brockdorff,Iga Grządzielewska,Remzi Karayol,Asifa Akhtar,Matthias Merkenschlager,Amanda G Fisher

Nature genetics 57:2264-2275 PubMed40890361

2025

Chromatin dynamics of a large-sized genome provides insights into polyphenism and X0 dosage compensation of locusts.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Liu,Feng Jiang,Ran Li,Shanlin Liu,Wenjing Fang,Xiao Li,Ting Hu,Lianyun Feng,Jie Zhang,Zhikang Liu,Jing He,Wei Guo,Xianhui Wang,Zongyi Sun,Jingjing Li,Yunan Gao,Jiacheng Yi,Qiye Li,Xiaoxiao Wang,Liya Wei,Le Kang

PeerJ 13:e19215 PubMed40183048

2025

Temporal and spatial distribution of histone acetylation in mouse molar development.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Du,Wanyi Luo,Liwei Zheng,Xuedong Zhou,Wei Du

Nature communications 16:2379 PubMed40064919

2025

Competitive antagonism of KAT7 crotonylation against acetylation affects procentriole formation and colorectal tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,Guanqun Mu,Bingquan Qiu,Shuo Wang,Changyu Tao,Yutong Mao,Xinhui Zhao,Jiansong Liu,Keyu Chen,Ziyu Li,Weibin Wang,Ence Yang,Yang Yang

Cellular and molecular neurobiology 45:19 PubMed39923205

2025

Organochlorine Pesticides and Epigenetic Alterations in Brain Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Yousefi,Gholamreza Asadikaram,Saeid Karamouzian,Moslem Abolhassani,Hossein Pourghadamyari,Vahid Moazed

Clinical epigenetics 17:20 PubMed39920865

2025

Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Yurong Chen,Haibo Zhu,Fucheng Guo,Luyao Wang,Wenli Zhang,Ruizhi Liu,Xiaoling Zhang,Xiangpeng Dai

PloS one 19:e0314329 PubMed39637191

2024

LARP7 is required for sex chromosome silencing during meiosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yukiko Tando,Atsuto Nonomura,Yumi Ito-Matsuoka,Asuka Takehara,Daiji Okamura,Yohei Hayashi,Yasuhisa Matsui

Scientific reports 14:21598 PubMed39285243

2024

Dynamics and necessity of SIRT1 for maternal-zygotic transition.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Nevoral,David Drutovic,Michaela Vaskovicova,Michal Benc,Frantisek Liska,Iveta Valentova,Sara Stachovicova,Jan Kubovciak,Jirina Havrankova,Miki Shavit,Ladan Monsef,Maria Iniesta-Cuerda,Tereza Zalmanova,Petr Hosek,Frantisek Strejcek,Milena Kralickova,Jaroslav Petr

View all publications

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chicCutRunSequencingBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H4 (acetyl K16) antibody [EPR1004]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Flow Cytometry (Intracellular) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

ChIC/CUT&RUN sequencing - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

ChIC/CUT&RUN sequencing - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Western blot - Anti-Histone H4 (acetyl K16) antibody [EPR1004] (AB109463)

Related conjugates and formulations (4)

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