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Rabbit Polyclonal Histone H4 acetyl K8 + K12 antibody. Suitable for ChIP, Dot, WB, ICC/IF, ChIP-seq and reacts with Human, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H4C1 acetyl K8 + K12 conjugated to Keyhole Limpet Haemocyanin.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.05% Proclin 300, 0.05% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human H4C1 acetyl K8 + K12 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P62805
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPDotWBICC/IFChIP-seq
Human
Tested
Expected
Tested
Tested
Tested
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Use 0.5 - 1 μg per IP.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Use 0.5 μg.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

Recommended products

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Rabbit Polyclonal Histone H4 acetyl K8 + K12 antibody. Suitable for ChIP, Dot, WB, ICC/IF, ChIP-seq and reacts with Human, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H4C1 acetyl K8 + K12 conjugated to Keyhole Limpet Haemocyanin.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human H4C1 acetyl K8 + K12 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P62805
Purification technique

Affinity purification

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • ChIP - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (ab233193), expandable thumbnail

    ChIP - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (ab233193)

    ChIP assays were performed using human K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells, ab233193 and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Image shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  • ChIP-sequencing - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (ab233193), expandable thumbnail

    ChIP-sequencing - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (ab233193)

    ChIP was performed with 0.5 μg ab233193 on sheared chromatin from 100,000 K562 cells. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete length of chromosome 2 (figure A) and a zoomin to a 600 kb region (figure B). Figure C and D show the enrichment in two genomic regions on chromosome 3 and 12, respectively, containing EIF4A2 and GAPDH positive controls.

  • Western blot - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (ab233193), expandable thumbnail

    Western blot - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (ab233193)

    Dilution buffer: TBS-Tween containing 5% skimmed milk.

    All lanes: Western blot - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (AB233193) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 25 µg

    Lane 2: HeLa histone extract at 15 µg

    Lane 3: Recombinant histone H2A at 1 µg

    Lane 4: Recombinant histone H2B at 1 µg

    Lane 5: Recombinant histone H3 at 1 µg

    Lane 6: Recombinant histone H4 at 1 µg

    Predicted band size: 11 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (ab233193), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (ab233193)

    HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were stained with ab233193 and with DAPI.

    Cells were fxed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA.

    Figure A: Cells were immunofluorescently labeled with ab233193 (left) diluted 1/500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa®488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

    Figure B, C, D and E: Staining of the cells with ab233193 after incubation of the antibody with 10 ng/μl of the following blocking peptides: H4K5,8,12 unmodifed (B), H4K5,8,12ac (C), H2A.ZK5,7,11ac (D) and H4K5,8,12,16ac (E).

  • Dot Blot - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (ab233193), expandable thumbnail

    Dot Blot - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (ab233193)

    To test the cross reactivity of ab233193 a Dot Blot analysis was performed with peptides containing other histone modifcations and the unmodifed H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. ab233193 was used at a dilution of 1:20,000.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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