Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Rabbit Polyclonal Histone H4 acetyl K8 + K12 antibody. Suitable for ChIP, Dot, WB, ICC/IF, ChIP-seq and reacts with Human, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H4C1 acetyl K8 + K12 conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
Preservative: 0.05% Proclin 300, 0.05% Sodium azide
Constituents: PBS
Liquid
Polyclonal
ChIP | Dot | WB | ICC/IF | ChIP-seq | |
---|---|---|---|---|---|
Human | Tested | Expected | Tested | Tested | Tested |
Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Use 0.5 - 1 μg per IP. |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Use 0.5 μg. |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Histone H4, HIST1H4D, H4FB, H4/B, H4C8, H4C5, H4/J, H4FJ, HIST1H4E, H4/C, H4FC, HIST1H4F, H4C6, H4C4, HIST1H4C, H4FG, H4/G, HIST2H4B, H4FO, H4C3, HIST1H4B, H4FI, H4/I, H4C2, HIST1H4A, H4FA, H4/A, H4C1, H4FH, HIST4H4, HIST1H4L, H4-16, H4C16, H4FK, H4C14, H4/N, H4F2, HIST2H4A, H4C15, H4/K, H4C13, HIST1H4K, H4FD, H4FN, HIST2H4, H4FM, H4/M, H4C9, HIST1H4I, H4C11, HIST1H4H, H4/E, H4FE, H4/O, HIST1H4J, H4C12, H4/D, H4/H
Rabbit Polyclonal Histone H4 acetyl K8 + K12 antibody. Suitable for ChIP, Dot, WB, ICC/IF, ChIP-seq and reacts with Human, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H4C1 acetyl K8 + K12 conjugated to Keyhole Limpet Haemocyanin.
Histone H4, HIST1H4D, H4FB, H4/B, H4C8, H4C5, H4/J, H4FJ, HIST1H4E, H4/C, H4FC, HIST1H4F, H4C6, H4C4, HIST1H4C, H4FG, H4/G, HIST2H4B, H4FO, H4C3, HIST1H4B, H4FI, H4/I, H4C2, HIST1H4A, H4FA, H4/A, H4C1, H4FH, HIST4H4, HIST1H4L, H4-16, H4C16, H4FK, H4C14, H4/N, H4F2, HIST2H4A, H4C15, H4/K, H4C13, HIST1H4K, H4FD, H4FN, HIST2H4, H4FM, H4/M, H4C9, HIST1H4I, H4C11, HIST1H4H, H4/E, H4FE, H4/O, HIST1H4J, H4C12, H4/D, H4/H
IgG
Rabbit
Preservative: 0.05% Proclin 300, 0.05% Sodium azide
Constituents: PBS
Liquid
Polyclonal
Affinity purification
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ChIP assays were performed using human K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells, ab233193 and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Image shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP was performed with 0.5 μg ab233193 on sheared chromatin from 100,000 K562 cells. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete length of chromosome 2 (figure A) and a zoomin to a 600 kb region (figure B). Figure C and D show the enrichment in two genomic regions on chromosome 3 and 12, respectively, containing EIF4A2 and GAPDH positive controls.
Dilution buffer: TBS-Tween containing 5% skimmed milk.
All lanes: Western blot - Anti-Histone H4 (acetyl K5 + K8 + K12) antibody (AB233193) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 25 µg
Lane 2: HeLa histone extract at 15 µg
Lane 3: Recombinant histone H2A at 1 µg
Lane 4: Recombinant histone H2B at 1 µg
Lane 5: Recombinant histone H3 at 1 µg
Lane 6: Recombinant histone H4 at 1 µg
Predicted band size: 11 kDa
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were stained with ab233193 and with DAPI.
Cells were fxed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA.
Figure A: Cells were immunofluorescently labeled with ab233193 (left) diluted 1/500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa®488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Figure B, C, D and E: Staining of the cells with ab233193 after incubation of the antibody with 10 ng/μl of the following blocking peptides: H4K5,8,12 unmodifed (B), H4K5,8,12ac (C), H2A.ZK5,7,11ac (D) and H4K5,8,12,16ac (E).
To test the cross reactivity of ab233193 a Dot Blot analysis was performed with peptides containing other histone modifcations and the unmodifed H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. ab233193 was used at a dilution of 1:20,000.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com