Rabbit Recombinant Monoclonal Histone H4 acetyl K8 + K12 + K16 + K5 + K8 + K12 + K16 + K5 + K8 + K12 + K16 + K8 + K12 + K16 antibody. Carrier free. Suitable for ICC/IF, WB, PepArr, IHC-P and reacts with Human, Mouse, Rat, Synthetic peptide - Human samples. Cited in 1 publication.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (AB240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunocytochemistry-immunofluorescence-img91846.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (AB240201)")
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (AB240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunocytochemistry-immunofluorescence-img62224.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (AB240201)")
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (AB240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunocytochemistry-immunofluorescence-img92478.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (AB240201)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (AB240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img31970.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (AB240201)")
![Peptide Array - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (AB240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--peptide-array-img71891.jpg/_jcr_content/renditions/webp-475.webp "Peptide Array - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (AB240201)")
Publications
Filter by
- Signal transduction and targeted therapy 6:3752021The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXujun Liu,Wenzhe Si,Lin He,Jianguo Yang,Yani Peng,Jie Ren,Xiaoping Liu,Tong Jin,Huajing Yu,Zihan Zhang,Xiao Cheng,Wenting Zhang,Lu Xia,Yunchao Huang,Yue Wang,Shumeng Liu,Lin Shan,Yu Zhang,Xiaohan Yang,Haixia Li,Jing Liang,Luyang Sun,Yongfeng ShangPubMed 34728602
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | PepArr | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected | Tested |
| Rat | Expected | Expected | Expected | Tested |
| Synthetic peptide - Human | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
PepArr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
2 products for Alternative Version
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4
Recommended products
Rabbit Recombinant Monoclonal Histone H4 acetyl K8 + K12 + K16 + K5 + K8 + K12 + K16 + K5 + K8 + K12 + K16 + K8 + K12 + K16 antibody. Carrier free. Suitable for ICC/IF, WB, PepArr, IHC-P and reacts with Human, Mouse, Rat, Synthetic peptide - Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR16606
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240201 is the carrier-free version of Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunocytochemistry-immunofluorescence-img91846.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)
Clone EPR16606 (ab240201) has been successfully conjugated by Abcam. This image was generated using Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab223996 for protocol details.
Alexa Fluor® 647 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab223996 staining Histone H4 (acetyl K5 + K8 + K12 + K16) in HeLa cells +/- Trichostatin A (500ng/ml, 4 hours). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.
The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab223996 at 1/200 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunocytochemistry-immunofluorescence-img62224.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)
Clone EPR16606 (ab240201) has been successfully conjugated by Abcam. This image was generated using Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab223995 for protocol details.
Alexa Fluor® 488 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab223995 staining Histone H4 (acetyl K5 + K8 + K12 + K16) in HeLa cells +/- Trichostatin A (500ng/ml, 4 hours). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.
The cells were then incubated overnight at +4°C with Alexa Fluor® 488 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab223995 at 1/200 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunocytochemistry-immunofluorescence-img92478.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790 at 1/5000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Nuclear staining on HeLa cell line is observed. The expression increased after treatment with TSA (50 μg/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
1. Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790 at 1/15000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img31970.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of Human colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Peptide Array - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--peptide-array-img71891.jpg "Peptide Array - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)")
Peptide Array - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)
Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, is available under the Support & downloads section.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49219.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of mouse colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56356.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of rat colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] ab177790).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49219.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-acetyl-k5-k8-k12-k16-antibody-epr16606-bsa-and-azide-free-ab240201--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56356.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - BSA and Azide free (ab240201)")
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