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Rabbit Polyclonal Histone H4 antibody. Suitable for IHC-P, IP, ChIP, WB and reacts with Human, Mouse, Rat samples. Cited in 300 publications.


Images

Immunoprecipitation - Anti-Histone H4 antibody - ChIP Grade (AB10158), expandable thumbnail
  • Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody - ChIP Grade (AB10158), expandable thumbnail
  • Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158), expandable thumbnail
  • ChIP - Anti-Histone H4 antibody - ChIP Grade (AB10158), expandable thumbnail

Publications

  • Nature cell biology 25:1821-18322023
    Prostate lineage-specific metabolism governs luminal differentiation and response to antiandrogen treatment.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Jenna M Giafaglione,Preston D Crowell,Amelie M L Delcourt,Takao Hashimoto,Sung Min Ha,Aishwarya Atmakuri,Nicholas M Nunley,Rachel M A Dang,Mao Tian,Johnny A Diaz,Elisavet Tika,Marie C Payne,Deborah L Burkhart,Dapei Li,Nora M Navone,Eva Corey,Peter S Nelson,Neil Y C Lin,Cedric Blanpain,Leigh Ellis,Paul C Boutros,Andrew S Goldstein
    PubMed 38049604
  • Science advances 9:eadi72382023
    Cla4 phosphorylates histone methyltransferase Set1 to prevent its degradation by the APC/C complex.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Xuanyunjing Gong,Shanshan Wang,Qi Yu,Min Wang,Feng Ge,Shanshan Li,Xilan Yu
    PubMed 37774018

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPChIPWB
Human
Expected
Predicted
Tested
Tested
Mouse
Predicted
Expected
Expected
Tested
Rat
Predicted
Predicted
Expected
Tested
Caenorhabditis elegans
Predicted
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Predicted
Drosophila melanogaster
Predicted
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Predicted
Saccharomyces cerevisiae
Predicted
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Predicted

Expected
Expected

Species

Human

Dilution info

1 µg/mL

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Chicken, Cow, Human, Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

2 µg chromatin for 25 µg chromatin

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Species

Rat

Dilution info

1 µg/mL

Notes

-

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Chicken, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster

Dilution info

-

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

Recommended products

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Rabbit Polyclonal Histone H4 antibody. Suitable for IHC-P, IP, ChIP, WB and reacts with Human, Mouse, Rat samples. Cited in 300 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Specificity

From May 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

For recombinant monoclonal Histone H4 antibody (ChIP grade) - please see Anti-Histone H4 antibody [IGX4696H] - ChIP Grade ab213291 (IGX4696H).

For mouse monoclonal Histone H4 antibody (ChIP grade) - please see Anti-Histone H4 antibody [mAbcam 31830] - ChIP Grade ab31830 (mAbcam 31830).

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-Histone H4 antibody - ChIP Grade (ab10158), expandable thumbnail

    Immunoprecipitation - Anti-Histone H4 antibody - ChIP Grade (ab10158)

    Histone H4 was immunoprecipitated using 0.5mg NIH/3T3 whole cell lysate, 5μg of Rabbit polyclonal to Histone H4 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10158.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 14kDa: Histone H4; non specific - 52 and 85kDa: We are unsure as to the identity of this extra band.

    All lanes: Immunoprecipitation - Anti-Histone H4 antibody - ChIP Grade (ab10158)

  • Western blot - Anti-Histone H4 antibody - ChIP Grade (ab10158), expandable thumbnail

    Western blot - Anti-Histone H4 antibody - ChIP Grade (ab10158)

    All lanes: Western blot - Anti-Histone H4 antibody - ChIP Grade (ab10158) at 1 µg/mL

    Lanes 1 and 3: Histone prep at 20 µg

    Lane 2: HeLa histone lysate at 20 µg

    Lane 4: HeLa Histone lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution

    Performed under reducing conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody - ChIP Grade (ab10158), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody - ChIP Grade (ab10158)

    IHC image of Histone H4 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10158, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Western blot - Anti-Histone H4 antibody - ChIP Grade (ab10158), expandable thumbnail

    Western blot - Anti-Histone H4 antibody - ChIP Grade (ab10158)

    All lanes: Western blot - Anti-Histone H4 antibody - ChIP Grade (ab10158) at 1 µg/mL

    Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg

    Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

  • ChIP - Anti-Histone H4 antibody - ChIP Grade (ab10158), expandable thumbnail

    ChIP - Anti-Histone H4 antibody - ChIP Grade (ab10158)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab10158 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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